CAZyme Information: sr12187-t26_1-p1

Basic Information

• Species: Sporisorium reilianum
• Lineage: Basidiomycota; Ustilaginomycetes; ; Ustilaginaceae; Sporisorium; Sporisorium reilianum
• CAZyme ID: sr12187-t26_1-p1
• CAZy Family: GH15
• CAZyme Description: related to cell wall protein UTR2

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
733		78572.83	6.9669

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SreilianumSRZ2	6791	999809	118	6673

• Gene Location: location=FQ311441:205309-207510(-)

Full Sequence      

MARSRRRAALRLSASLLAGLVLASTLPSTSAQTSRITCSTSSLCPESSPCCSSEGVCGSG
AMQCAGGCDPMSSFKPSSCLPNPVCRSRQMTIKPSDYNDNSVFMPILEYNGNASSPFTLD
SGSLGAGPEGVLLQLSAAQQAKISTTDYLMYGYVEATLRHNARQGLVAAFIFMSNIKDEV
DWEFTTSNSSLGLTNYFWMGQPVRDHGVNVTTPNFNVSDWHTYGLNWTSSQLQWSIDGTV
VRTLTRAQAGTSYPRSPSRIQFSTWAGGNETNPQGTIDWAGGPIDWNSPEYKKNGFYSQE
IKQFNVQCASLGSLNLTSGSGSSNGNITSYVYTGGKSTTTLEPAFGTSTDPLRILSDPAA
DGYPGYPGYGANSAQSGKDGKNSKDSKDAKSSSKDSNNHSKDGDANAKDDAAANSSSTST
YGCALPISGAIIGLTAIWAVIAFVRRRHLKAPIIINAIGVTGVNDARFSGPSRSGFERSS
NTATALAYHDLENEPASMYCNEPNMNLMMGGGIMSDAGLSHLQRQTTGGSKVSRTSSRSV
ADRLLGTSGRAQRYQQLDTTMEEEAMDQVSRQRPYLAGANPYQHHAYEQAAREEEDEVEE
YVAGHAHQTYYGTPISSPVKRNATVATSYYEPNTSAADQSFEACQLQSPQRRALPITPYT
PNYAVSPSAPPPRPSGRMYDTPNVNYYPTPVMMQSDGYVPNVHAPAAAPYTHISGGAGYY
TGGYAPMPPPRHR

Enzyme Prediction     

No EC number prediction in sr12187-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	133	285	1.6e-54	0.9137931034482759

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185692	GH16_fungal_CRH1_transglycosylase	2.29e-75	108	310	2	203	glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
395585	Glyco_hydro_16	2.56e-35	133	281	13	168	Glycosyl hydrolases family 16.
185683	Glyco_hydrolase_16	9.70e-23	144	279	60	206	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
211314	ChtBD1_GH16	1.04e-18	36	83	1	47	Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 16 glycosyl hydrolases. This subfamily includes Saccharomyces cerevisiae Utr2p, also known as Crh2p, which participates in the cross-linking of chitin to beta(1-3)- and beta(1-6) glucan in the cell wall, and S. cerevisiae Crr1p, a putative transglycosidase which is needed for proper spore wall assembly. ChtBD1 is a lectin domain found in proteins from plants and fungi that bind N-acetylglucosamine, plant endochitinases, wound-induced proteins, and the alpha subunit of Kluyveromyces lactis killer toxin. This domain is involved in the recognition and/or binding of chitin subunits; it typically occurs N-terminal to glycosyl hydrolase domains in chitinases, together with other carbohydrate-binding domains, or by itself in tandem-repeat arrangements.
185684	GH16_lichenase	1.34e-15	141	289	58	200	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBQ70290.1|CBM18|GH16_19	0.0	1	733	1	733
SJX60964.1|CBM18|GH16_19	0.0	1	733	1	727
CDR88212.1|CBM18|GH16_19	0.0	1	732	1	745
SHO79225.1|CBM18|GH16	1.75e-56	67	364	49	344
AXA49093.1|CBM18|GH16	1.54e-55	61	364	46	347

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6IBU_A	5.16e-31	112	309	30	224	Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBU_B Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBW_A Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293],6IBW_B Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293]
5SV8_A	1.27e-06	171	265	76	171	Crystal Structure of the catalytic nucleophile and surface cysteine mutant of VvEG16 in complex with a xyloglucan oligosaccharide [Vitis vinifera]
3WVJ_A	1.48e-06	145	303	68	220	Chain A, Beta-glucanase [Acetivibrio thermocellus ATCC 27405],3WVJ_B Chain B, Beta-glucanase [Acetivibrio thermocellus ATCC 27405]
5DZE_A	7.52e-06	171	265	75	170	Crystal Structure of the catalytic nucleophile mutant of VvEG16 in complex with cellotetraose [Vitis vinifera]
5DZF_A	7.61e-06	171	265	76	171	Crystal Structure of the catalytic nucleophile mutant of VvEG16 in complex with a mixed-linkage glucan octasaccharide [Vitis vinifera],5DZF_B Crystal Structure of the catalytic nucleophile mutant of VvEG16 in complex with a mixed-linkage glucan octasaccharide [Vitis vinifera]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WI46|CRF2_ASPFU	1.61e-42	38	308	27	300	Probable glycosidase crf2 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=crf2 PE=1 SV=1
sp|Q5AJC0|UTR2_CANAL	9.51e-40	37	303	28	297	Extracellular glycosidase UTR2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=UTR2 PE=1 SV=1
sp|P32623|CRH2_YEAST	2.39e-34	38	308	27	300	Probable glycosidase CRH2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UTR2 PE=1 SV=3
sp|Q8J0P4|CRF1_ASPFU	5.84e-29	112	309	51	245	Probable glycosidase crf1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=crf1 PE=1 SV=2
sp|Q75A41|CRR1_ASHGO	4.53e-27	36	303	29	356	Probable glycosidase CRR1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=CRR1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.002237	0.997734	CS pos: 31-32. Pr: 0.9158

TMHMM  Annotations     

There is no transmembrane helices in sr12187-t26_1-p1.