CAZyme Information: mRNA_M_BR32_EuGene_00110881-p1

Basic Information

• Species: Pyricularia oryzae
• Lineage: Ascomycota; Sordariomycetes; ; Pyriculariaceae; Pyricularia; Pyricularia oryzae
• CAZyme ID: mRNA_M_BR32_EuGene_00110881-p1
• CAZy Family: GH5
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
912	BR32_scaffold00016|CGC3	102542.34	5.2031

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PoryzaeBR32	14781	N/A	432	14349

• Gene Location: location=BR32_scaffold00016:258892-261630(+)

Full Sequence      

MSVTIQDLATGWEFKATSRQPDDVTQAAAFDSWHPVHSVPTEVHLDLHRSGLIPDPFVDL
NELSVRWVADQQWTYRCQFASPASRASSSITDLVFQGLDTFATVSLNGEVILNSDNMFIS
HRVNITDKLRPDGALNDLGITFESARLRGLELVKEHPEHRHIVHQTEIGRGPVRKAQYQW
GWDWGPIMLTCGPWKPVLLETYSVRIDDLHVTYLLEDGLDRADVSINFKTRGLLPGAGVS
YEVLIDGENVGSSTTDACGDGELELVIKTPKLWWPRGYGDQNLYALKVRLLSDDKTVLDS
VTKKIGLRTVELVQEKDAIGQSFYFRINDVDMFCGGACWIPSDSFLTRTTPSQYRDWITR
HVAEGNQAMIRVWGGGIYEADAFFDACDEAGVLAWQDFCFACANYPAHPGFLQSVEAEAR
QNVRRLKHHPSLVAWAGNNEDYQIVERYWLQYDFAGDKDPQSWLRTNFPARYIYEHLLPL
IVGEEYGASKNQSLVAYHPSSPWGNGTSTTLVVDQTVGDVHQWNVWHGAMRPYQLLPEMG
GRFVSEFGMEAYPHVSTLAGAVTDPRQRRPGSMAMDFRNKAGGHTRRLLAYVGENFDLHG
TGGTLEGFAYLTQVMQADAMRAAYRGWRRQWSGRRCGGVLVWQLNDCWPTVSWAVIDYRG
VRKPAWYAIRDALKPVAVGVGRRFFDCTNRPADRTWQRDTGHVDPRLAWGSSERPEELLS
DLWVANGTTKDVSGRLTVSFVSVKSGRVLKELYDTPLDILAKANDITEVEVKQSLPAAEG
RGVFNPAQEDPFIIHARLELLGDDGQVVETVEDVTWPDPIKWLDFDDRKVQVSARVEGEQ
AVLTIRAERPVKAFVIQENPDLRVSENGFDLVPGYPATVKVDGCSDVKKLVWRYHGQVGL
GDEWAENPEDGI

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	737	2.2e-94	0.6954787234042553

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	5.39e-70	5	678	12	634	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
395572	Glyco_hydro_2	4.31e-14	204	308	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
407658	Mannosidase_ig	6.13e-10	721	821	4	90	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
236657	PRK10150	2.12e-09	195	440	172	417	beta-D-glucuronidase; Provisional
407625	Ig_mannosidase	0.001	829	881	3	56	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBZ66458.1|GH2	0.0	1	912	1	912
AEO59187.1|GH2	0.0	4	897	5	897
QRD07513.1|GH2	0.0	4	896	10	879
AEO64472.1|GH2	0.0	4	897	5	881
CBX98985.1|CBM35|GH2|GH27	1.53e-306	8	894	622	1494

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	4.91e-132	2	683	23	663	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	2.83e-112	8	678	11	656	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.97e-112	8	678	13	658	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	3.03e-112	8	678	13	658	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	7.86e-112	8	678	11	656	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5B7W2|MANBB_EMENI	1.18e-268	8	897	9	843	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
sp|Q0CCA0|MANBB_ASPTN	2.13e-258	6	897	7	843	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
sp|A2QYN2|MANBB_ASPNC	1.14e-255	8	897	9	844	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
sp|Q2TXB7|MANBB_ASPOR	1.47e-253	8	897	9	844	Beta-mannosidase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndB PE=3 SV=3
sp|B8NW36|MANBB_ASPFN	2.08e-253	8	897	9	844	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000068	0.000000

TMHMM  Annotations     

There is no transmembrane helices in mRNA_M_BR32_EuGene_00110881-p1.