CAZyme Information: mRNA_M_BR32_EuGene_00077441-p1

Basic Information

• Species: Pyricularia oryzae
• Lineage: Ascomycota; Sordariomycetes; ; Pyriculariaceae; Pyricularia; Pyricularia oryzae
• CAZyme ID: mRNA_M_BR32_EuGene_00077441-p1
• CAZy Family: GH17
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
659	BR32_scaffold00008|CGC10	74523.88	5.8155

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PoryzaeBR32	14781	N/A	432	14349

• Gene Location: location=BR32_scaffold00008:748203-750667(+)

Full Sequence      

MQLIRILAAFSGITLRMVNAKLDAREQGGKYVLANDRLYTEIDKTNGVMTSVQLDGMDML
GRPSGSTGQGPYLDCYCTPSGFWTPGTHRPEYAFWSDNATWAGVRMRDTHQDTGQIMEQY
WFIRDGETGLHTFSRVAYYNETHPFLRNLQELRTLFRPSTDIYTHLVTNERQFAPLPGVE
AVKNQVVVQDATWWLGNTTNDAYVQKESDYFTKYTFMDVWKDHKAHGMFADGTKTPDNTT
YGAFLVHNTVDTYFGGPLHSDLVVDGIVYNYMVSNHHGEQTPNITNGFDRTFGPSFFYFN
HGPAGASIHDLRQDAEQYADPTWNAEFYDVIAPFVTNYVPTSSRSTFRLHVDLPQGAQNP
VAILTENHHYHQDNVYDTKALQYWTRISPNGDAAIPRVAHGSYRLTIIADGIFGDYVKDD
VVVSVSPTAPRTHARWREESAGTEIFRIGTPDKSSGEFKHGYEPDQTKPLQPEQYRIYWG
AYDFVEDFPNGVTFKVGESKEVDDMNYIHWSVFGGRGNHRRPEPYFGNGDVNNWTIKFDV
NEENIQKKREATFTVQLAGAKTAAGNTDVFNASEPHANLAYTVNVNGADLAPWIIPSCGV
RSAVICFNLAHKFTFDPTLLKPGENKIVLSLPYAAQNYESALLTNSVYVQYDALRLEIR

Enzyme Prediction     

EC	4.2.2.23:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL4	20	643	7.3e-287	0.995253164556962

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
199907	RGL4_N	2.99e-52	25	304	4	261	N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
199905	RGL4_C	3.28e-28	446	657	1	161	C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
199904	RGL4_M	5.44e-17	343	416	1	74	Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
405387	fn3_3	2.54e-16	354	416	1	60	Polysaccharide lyase family 4, domain II. FnIII-like is domain II of rhamnogalacturonan lyase (RG-lyase). The full-length protein specifically recognizes and cleaves alpha-1,4 glycosidic bonds between l-rhamnose and d-galacturonic acids in the backbone of rhamnogalacturonan-I, a major component of the plant cell wall polysaccharide, pectin. This domain displays an immunoglobulin-like or more specifically Fibronectin-III type fold and shows highest structural similarity to the C-terminal beta-sandwich subdomain of the pro-hormone/propeptide processing enzyme carboxypeptidase gp180 from duck. It serves to assist in producing the deep pocket, with domain III, into which the substrate fits.
405384	CBM-like	2.02e-15	445	656	2	157	Polysaccharide lyase family 4, domain III. CBM-like is domain III of rhamnogalacturonan lyase (RG-lyase). The full-length protein specifically recognizes and cleaves alpha-1,4 glycosidic bonds between l-rhamnose and d-galacturonic acids in the backbone of rhamnogalacturonan-I, a major component of the plant cell wall polysaccharide, pectin. This domain possesses a jelly roll beta-sandwich fold structurally homologous to carbohydrate binding modules (CBMs), and it carries two sulfate ions and a hexa-coordinated calcium ion.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBZ58754.1|PL4_3	0.0	1	659	1	664
CEI69636.1|PL4_3	0.0	16	658	19	669
QKD49944.1|PL4_3	0.0	16	659	21	672
QGI80329.1|PL4_3	0.0	16	658	19	669
QGI63162.1|PL4_3	0.0	16	658	19	669

PDB Hits     

mRNA_M_BR32_EuGene_00077441-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5AZ85|RGLB_EMENI	2.35e-248	17	659	15	660	Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2
sp|B8N5T6|RGLB_ASPFN	1.39e-245	17	658	16	662	Probable rhamnogalacturonate lyase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rglB PE=3 SV=1
sp|Q2U5P7|RGLC_ASPOR	1.71e-244	20	650	21	654	Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglC PE=3 SV=1
sp|Q0C7K7|RGLB_ASPTN	2.90e-244	22	658	21	659	Probable rhamnogalacturonate lyase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=rglB PE=3 SV=1
sp|Q5B5P1|RGLC_EMENI	6.69e-237	20	650	21	650	Probable rhamnogalacturonate lyase C OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglC PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.002785	0.997180	CS pos: 20-21. Pr: 0.9352

TMHMM  Annotations     

There is no transmembrane helices in mRNA_M_BR32_EuGene_00077441-p1.