CAZyme Information: jhhlp_008309-t41_1-p1

Basic Information

• Species: Lomentospora prolificans
• Lineage: Ascomycota; Sordariomycetes; ; Microascaceae; Lomentospora; Lomentospora prolificans
• CAZyme ID: jhhlp_008309-t41_1-p1
• CAZy Family: GT32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
394	NLAX01001623|CGC13	43889.86	4.9808

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_LprolificansJHH5317	8768	N/A	208	8560

• Gene Location: location=NLAX01001623:319383-320682(+)

Full Sequence      

MPQRTVAYFVNWAIYGRKHRPQDLPAENLTHILYAFANIKPDGEVHLTDTWADTDIHWEG
DSWNDVGTNLYGCLKQLNLLKKHNRNLKVLMSIGGWTYSPNFKGPASTPEGRKRFADTSI
ELVKNLGLDGIDIDWEYPANPQEAADYVELLRVCRQALDEYSKTLPNPYHFELTVASPCG
TENYQRMDLRGMDQYLDFWNLMAYDYAGSWDSVAAHQANLYPSGSNPKSTPFSTQAAVDY
YINQAGIAPNKIVIGMPLYGRAFENTDGPGTPYQGVGQGTWEQGVYDFKKLPLDGASEVD
DPSIGASYCFNSGTRTFVSYDTVQTAKTKAEYIKSRDLGGAMWWESSADKEGQQSIIGNV
TNAFGGPGGLLKGDNCITYPHTKYDNLKNGFPGQ

Enzyme Prediction     

EC	3.2.1.14:25

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	3	356	6.1e-82	0.9594594594594594

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119365	GH18_chitinase	9.27e-134	6	349	2	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
214753	Glyco_18	6.70e-128	4	349	1	334	Glyco_18 domain.
225862	ChiA	1.36e-98	2	352	37	426	Chitinase, GH18 family [Carbohydrate transport and metabolism].
395573	Glyco_hydro_18	1.85e-91	4	349	1	307	Glycosyl hydrolases family 18.
119351	GH18_chitolectin_chitotriosidase	1.54e-76	6	344	2	336	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CEI70245.1|GH18	3.92e-224	4	392	9	397
AAP01160.1|GH18	3.92e-224	4	392	9	397
CCU56514.1|GH18	1.07e-223	4	392	74	463
CZS85519.1|GH18	1.12e-223	4	392	9	397
CEF87375.1|GH18	1.12e-223	4	392	9	397

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1D2K_A	1.78e-184	4	392	4	391	Chain A, CHITINASE 1 [Coccidioides immitis],1LL4_A Chain A, Chitinase 1 [Coccidioides immitis],1LL4_B Chain B, Chitinase 1 [Coccidioides immitis],1LL4_C Chain C, Chitinase 1 [Coccidioides immitis],1LL4_D Chain D, Chitinase 1 [Coccidioides immitis]
1LL7_A	5.10e-184	4	392	4	391	Chain A, CHITINASE 1 [Coccidioides immitis],1LL7_B Chain B, CHITINASE 1 [Coccidioides immitis]
1LL6_A	1.03e-183	4	392	4	391	Chain A, CHITINASE 1 [Coccidioides immitis],1LL6_B Chain B, CHITINASE 1 [Coccidioides immitis],1LL6_C Chain C, CHITINASE 1 [Coccidioides immitis],1LL6_D Chain D, CHITINASE 1 [Coccidioides immitis]
1WNO_A	2.25e-176	4	390	6	392	Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407 [Aspergillus fumigatus],1WNO_B Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407 [Aspergillus fumigatus]
6IGY_A	2.70e-176	4	391	17	403	Crystal structure of Aspergillus niger chitinase B [Aspergillus niger]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q1E3R8|CHI1_COCIM	3.18e-183	4	392	39	426	Endochitinase 1 OS=Coccidioides immitis (strain RS) OX=246410 GN=CTS1 PE=3 SV=1
sp|P0CB51|CHI1_COCPS	3.18e-183	4	392	39	426	Endochitinase 1 OS=Coccidioides posadasii (strain RMSCC 757 / Silveira) OX=443226 GN=CTS1 PE=1 SV=1
sp|C5P230|CHI1_COCP7	1.29e-182	4	392	39	426	Endochitinase 1 OS=Coccidioides posadasii (strain C735) OX=222929 GN=CTS1 PE=3 SV=1
sp|G5EAZ3|CHIB1_EMENI	1.53e-181	4	392	5	396	Endochitinase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=chiB PE=1 SV=1
sp|Q92222|CHIB_EMEND	1.53e-181	4	392	5	396	Endochitinase B OS=Emericella nidulans OX=162425 GN=chiB PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000051	0.000001

TMHMM  Annotations     

There is no transmembrane helices in jhhlp_008309-t41_1-p1.