CAZyme Information: jhhlp_002737-t41_1-p1

Basic Information

• Species: Lomentospora prolificans
• Lineage: Ascomycota; Sordariomycetes; ; Microascaceae; Lomentospora; Lomentospora prolificans
• CAZyme ID: jhhlp_002737-t41_1-p1
• CAZy Family: CBM50
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
442		48777.04	4.6678

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_LprolificansJHH5317	8768	N/A	208	8560

• Gene Location: location=NLAX01000008:3876151-3877588(-)

Full Sequence      

MAIELQITQEYLGQQAHLFYLPPLWREILDFDMRWGGEDLRVKDILTGKRPGGKTVSKGG
FAAVSNVGQEKSWLGSHLAMSNLYTYGRLAWNPWDEPVAMLHDWTRLTFGLDEDVRDVIT
EMSMESWPAYENYTGNLGVQTLTGITGNHYGPNPASQDGNGWGQWTRADRNGIGMDRTVA
TGTGNAGQYPPEVAAMYEDVETTPENLILWFHHLPWTHKLSTGKTIIQHFYDAHYAGAET
AQTFPLLWQTLKGKIDDERYEAQLYRLEYQAGHSVVWRDFICRFYNKMSGIQDEQDRVNN
HVWRIEAESMTLSGYSRANANPSEAASGSVVTAGGGGGSASAEIPFDSGVYDIAVGYFDV
SNGKASWTISIGDEVIGEWQGDVESHISKASSNGLDGHSAARVTFRGVTVEKGQTVKITG
SPNGGEAAPLDYVSFQPEGILD

Enzyme Prediction     

EC	3.2.1.131:5	3.2.1.139:5

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH67	2	297	2.3e-116	0.4334828101644245

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
400039	Glyco_hydro_67C	3.55e-137	75	298	2	223	Glycosyl hydrolase family 67 C-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the C terminal region of alpha-glucuronidase which is mainly alpha-helical. It wraps around the catalytic domain (pfam07488), making additional interactions both with the N-terminal domain (pfam03648) of its parent monomer and also forming the majority of the dimer-surface with the equivalent C-terminal domain of the other monomer of the dimer.
226187	AguA2	2.94e-97	3	297	390	679	Alpha-glucuronidase [Carbohydrate transport and metabolism].
271143	CBM6-CBM35-CBM36_like	7.62e-05	304	435	1	124	Carbohydrate Binding Module 6 (CBM6) and CBM35_like superfamily. Carbohydrate binding module family 6 (CBM6, family 6 CBM), also known as cellulose binding domain family VI (CBD VI), and related CBMs (CBM35 and CBM36). These are non-catalytic carbohydrate binding domains found in a range of enzymes that display activities against a diverse range of carbohydrate targets, including mannan, xylan, beta-glucans, cellulose, agarose, and arabinans. These domains facilitate the strong binding of the appended catalytic modules to their dedicated, insoluble substrates. Many of these CBMs are associated with glycoside hydrolase (GH) domains. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold. CBM36s are calcium-dependent xylan binding domains. CBM35s display conserved specificity through extensive sequence similarity, but divergent function through their appended catalytic modules. This alignment model also contains the C-terminal domains of bacterial insecticidal toxins, where they may be involved in determining insect specificity through carbohydrate binding functionality.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SAL89189.1|GH67	4.99e-197	2	442	395	835
QPC59691.1|GH67	5.31e-193	2	442	406	843
CZS81762.1|GH67	1.06e-192	2	442	406	843
QGA17702.1|GH67	1.10e-192	2	442	402	844
CEF78469.1|GH67	2.13e-192	2	442	406	843

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1K9D_A	6.30e-85	3	297	385	675	The 1.7 A crystal structure of alpha-D-glucuronidase, a family-67 glycoside hydrolase from Bacillus stearothermophilus T-1 [Geobacillus stearothermophilus],1L8N_A The 1.5A crystal structure of alpha-D-glucuronidase from Bacillus stearothermophilus T-1, complexed with 4-O-methyl-glucuronic acid and xylotriose [Geobacillus stearothermophilus],1MQQ_A THE CRYSTAL STRUCTURE OF ALPHA-D-GLUCURONIDASE FROM BACILLUS STEAROTHERMOPHILUS T-1 COMPLEXED WITH GLUCURONIC ACID [Geobacillus stearothermophilus]
1K9E_A	6.30e-85	3	297	385	675	Chain A, alpha-D-glucuronidase [Geobacillus stearothermophilus],1K9F_A Chain A, alpha-D-glucuronidase [Geobacillus stearothermophilus]
1MQP_A	6.30e-85	3	297	385	675	The Crystal Structure Of Alpha-D-Glucuronidase From Bacillus Stearothermophilus T-6 [Geobacillus stearothermophilus]
1MQR_A	1.74e-84	3	297	385	675	Chain A, ALPHA-D-GLUCURONIDASE [Geobacillus stearothermophilus]
1GQI_A	1.39e-42	1	290	380	668	Structure of Pseudomonas cellulosa alpha-D-glucuronidase [Cellvibrio japonicus],1GQI_B Structure of Pseudomonas cellulosa alpha-D-glucuronidase [Cellvibrio japonicus],1GQJ_A Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with xylobiose [Cellvibrio japonicus],1GQJ_B Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with xylobiose [Cellvibrio japonicus],1GQK_A Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid [Cellvibrio japonicus],1GQK_B Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid [Cellvibrio japonicus],1GQL_A Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid and xylotriose [Cellvibrio japonicus],1GQL_B Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid and xylotriose [Cellvibrio japonicus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0CJP9|AGUA_ASPTN	1.84e-190	2	442	401	841	Probable alpha-glucuronidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=aguA PE=3 SV=1
sp|B8NGU1|AGUA_ASPFN	9.81e-190	1	442	398	839	Probable alpha-glucuronidase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=aguA PE=3 SV=1
sp|Q2UFP4|AGUA_ASPOR	2.46e-189	1	442	394	835	Probable alpha-glucuronidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=aguA PE=3 SV=1
sp|A1CC12|AGUA_ASPCL	1.48e-186	2	442	400	840	Probable alpha-glucuronidase A OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=aguA PE=3 SV=1
sp|Q5AQZ4|AGUA_EMENI	1.82e-186	2	442	407	847	Alpha-glucuronidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=aguA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000057	0.000000

TMHMM  Annotations     

There is no transmembrane helices in jhhlp_002737-t41_1-p1.