CAZyme Information: ZTRI_8.263.mRNA-p1

Basic Information

• Species: Zymoseptoria tritici
• Lineage: Ascomycota; Dothideomycetes; ; Mycosphaerellaceae; Zymoseptoria; Zymoseptoria tritici
• CAZyme ID: ZTRI_8.263.mRNA-p1
• CAZy Family: GT31
• CAZyme Description: similar to glucosidase ii alpha subunit

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
983	Ztri_chr_8|CGC5	111043.16	5.9522

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_ZtriticiIPO323	11839	336722	13	11826

• Gene Location: location=Ztri_chr_8:909292-912316(+)

Full Sequence      

MRGSNGSWTMWSNLLFLLATICSLITPAVTVKHENFKTCDQSGFCKRNRLYADNALSTST
WESPYQLEPSTIKFNDGILTGIVNKKLASSETIRLPFKLAFYESGVARVTIDEEKRQKGE
IELRHDSKARKERYNEAEKWAIVGGTTVSTTAAISKDADKDTTKVVYGAGSKFDAIIRHS
PLSIDFRRDGEIQIKFNDKGLLNVEHWRRKVEKKAEEQNGTDDAAAKTEDKVAGEDESTW
WDEAFGGNTDTKPRGPESVAMDISFPGYDFVYGIAEHAGPLALKETRGGDGKYSEPYRMY
NADVFEYEMDSPMTLYGNIPFMQAQKKDSTVGVFWLNAAETWVDITKTSTLANAMSLGIA
GDKTTNTHWISENGLLDVFVFLGPTPQDVLGAYTELTGTQQLPQHFSIASHQCRWNYVTD
EDVRDVDKKFDKHRIPYDVIWLDIEYTDGKKYFTWDSMTFPDPLGMQKQLDEHDRKLVAI
IDPHIKNEGGYPIVDELKSKGLAVNNKEGNIYEGWCWPGSSHWVDCFSPAARKWWADLFQ
YAKFPGSAKNLFIWNDMNEPSVFNGPETTMPKDNLHHDNWEHRDVHNLNGMTLINATYEG
LLARSPAEQKQNVRPFVLTRSFFAGSQRLGAMWTGDNQAEWSHLAASIPMTLSMGISGFP
FAGADVGGFFGNPDKELLTRWYQAGIFYPFMRAHAHIDTRRREPYLAGSPYTEIITQAIR
LRYALLPAWYTAFHESHMTGAPIIRPHYYVFPGDEKGFAIDDQFFIGSTGLLAKPVTTPE
TTAQKIYLADKEVYYDYFDFWTYQGPGEVNVNAPLDVIPLLMRGGHIFPRRDRPRRSSGL
MKYDPVTLVVVLGHSGDAEGTLYLDDGESFDYEQGAFIHRSFKYTAASKELVSEDLTPKE
KKGKKQEAYKKVMKDVRVEKIVLVGVPDGWVGKTEVTVVEEGGKAERKVAVEVFAKEKEG
KAAYAVVRDPEVRVGEGWRVKFE

Enzyme Prediction     

EC	3.2.1.20:27	3.2.1.84:26

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	379	827	6.8e-153	0.9929742388758782

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224418	YicI	0.0	177	919	60	761	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
395838	Glyco_hydro_31	0.0	379	827	1	441	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
269889	GH31_GANC_GANAB_alpha	0.0	398	869	1	467	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.
269890	GH31_glucosidase_II_MalA	2.68e-147	398	740	1	339	Alpha-glucosidase II-like. Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This subgroup also includes the MalA alpha-glucosidase from Sulfolobus solfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source.
215408	PLN02763	2.22e-113	384	981	164	757	hydrolase, hydrolyzing O-glycosyl compounds

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SMQ53257.1|GH31	0.0	1	983	1	983
SMY26885.1|GH31	0.0	1	983	1	983
SMR59697.1|GH31	0.0	1	983	1	983
SMR56837.1|GH31	0.0	1	983	1	983
UJO19422.1|GH31	0.0	1	982	1	993

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5DKZ_A	0.0	31	982	5	950	Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495],5DL0_A Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495]
5DKX_A	0.0	31	982	5	950	Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495],5DKY_A Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495],5JQP_A Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495]
7KRY_A	2.32e-248	13	980	14	940	Chain A, Neutral alpha-glucosidase AB [Mus musculus],7KRY_C Chain C, Neutral alpha-glucosidase AB [Mus musculus]
5IEF_A	2.39e-248	31	980	1	909	Murine endoplasmic reticulum alpha-glucosidase II with N-butyl-1-deoxynojirimycin [Mus musculus]
5IEG_A	2.47e-248	31	980	1	909	Murine endoplasmic reticulum alpha-glucosidase II with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9US55|GLU2A_SCHPO	6.46e-281	15	884	10	849	Glucosidase 2 subunit alpha OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gls2 PE=1 SV=1
sp|Q9FN05|PSL5_ARATH	2.47e-252	13	981	3	919	Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1
sp|Q4R4N7|GANAB_MACFA	1.32e-249	11	980	15	941	Neutral alpha-glucosidase AB OS=Macaca fascicularis OX=9541 GN=GANAB PE=2 SV=1
sp|Q8BHN3|GANAB_MOUSE	2.64e-249	11	980	15	941	Neutral alpha-glucosidase AB OS=Mus musculus OX=10090 GN=Ganab PE=1 SV=1
sp|Q94502|GANAB_DICDI	1.62e-247	14	926	6	898	Neutral alpha-glucosidase AB OS=Dictyostelium discoideum OX=44689 GN=modA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000267	0.999719	CS pos: 30-31. Pr: 0.9591

TMHMM  Annotations     

Start	End
7	29