CAZyme Information: ZTRI_6.565.mRNA-p1

Basic Information

• Species: Zymoseptoria tritici
• Lineage: Ascomycota; Dothideomycetes; ; Mycosphaerellaceae; Zymoseptoria; Zymoseptoria tritici
• CAZyme ID: ZTRI_6.565.mRNA-p1
• CAZy Family: GT1
• CAZyme Description: similar to udp-glucose:glycoprotein glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1542		173379.13	4.9149

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_ZtriticiIPO323	11839	336722	13	11826

• Gene Location: location=Ztri_chr_6:1998878-2003557(+)

Full Sequence      

MAVLSGWKAGVLASLLAWANAVPSINVELRTSFGAPPYLVELLETAAEANASSYFPLLDR
IADGYFDSSKTDRDLYNSFRSVLQQDSHLSSEALSLFDFALSIHAAAPRIEAHYQYYNTS
IVPTLLADESEGCETWAHMSYKRQRYCSPELRESDVIKDYGPADDDVRSLPFDRVLGDDF
NEKPSVLYADIESEAFRKFHKKLSPTAKEGSTSYRVRYRATPGYESRPLAVSGYGVELAL
KRTDYIVIDDRQAEEAKSEDNAGDATLSEEELSDLRPLSSSELRRLGLKASSFVMASEDP
FETLLRLSSDFPKHSSPIAATNVSKEFLKEHEHNRELLLPAGYNVMWMNGLQVMSRDVEA
YALLEQLRRERKMINDVQSIGLTGAEAVELLSHEAITKSQVGQEAQRYDWRDDAEGGNVI
IWLNNIEKDKRYASWPATVNAFLQRTYPGQLPSVRKEVQNLVLPIDFSDLSDVSLVADSI
RGFVERQVPIQFGLVPRINSPASKEQAKVFYYLVDRYGVAVALDYLERSVAGSTRKLGGP
DEKHFTTAIAERILRRSKEALEFSKIADDEELSARLKPVQAYLARLGDIDAAPHAFINGV
PIPRTDEWLQVMSQRIGLDLRVVQQAIYEGTLSDDDYLPDVFLSKASFRRNNIVIPGDDK
TLRHLNLGELPHFELLPSLPASKDTIERELVHLTVAADFDTTEGFEQLMEALVFREEHDN
VELAVLHIPISPNPAIHMVAVLNKELIEQTLSGYIEQYKKLQGEVATGDNLSARREVYAS
MRKTAMTGDDINEPGFEVFLNHKLMNAIDKERDEKALVINGRVVGPFAQETVLEVADLEA
LFSYERKRRLLPAALAIESLKLQDKASTPLAFARVSNVIALSLISEVPEGIFEQAPSVRM
DTFKKWDSTYTAIRIGDEKTANIQIYAAVDPASEVAQRWIPIIKVLSELEGVYVCLYLNP
KERLEEIPIKRFYRHVISSKPDFEDDGSLRAARAQFGGLPADALLNMGMDLPPSWLVAPK
HTVHDLDNIKLSAVKSGSDIDAVYELEHILIEGHSKDASSGPPPRGVQLVLGTESDPHFA
DTIIMANLGYFQFKANPGIYNLALQKGRSEAIFNIDSTGALGYNVQSDDDFPSTEIALMS
FRGTTLFPELSRKPGMEDEDVLEASKSAFESLADNADKLLAQAGLKSGSAGKYLSKAAQL
GSGLFSSKDDVQAPKHADINIFSVASGHLYERMLNIMMVSVMKHSSHTVKFWFIEQFLSP
SFKDFLPIMATAYGFQYEMVTYKWPHWLRGQKEKQREIWGYKILFLDVLFPLDLDKVIFV
DADQIVRTDMYELVQHDLEGAPYGFTPMCDSRTEMEGFRFWKQGYWKNFLKGLPYHISAL
YVVDLKLFRQMAAGDRLRQNYHQLSADPNSLSNLDQDLPNHMQSLLPIHSLPQEWLWCET
WCSDESLKDAKTIDLCNNPQTKEPKLDRARRQVPEWTAYDDEIAGVAKRYKDSRSGGKDK
PDGAPDTSVPVVAQAGEESIQERLQREDAERERKKVKGHDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1219	1466	3.1e-133	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1219	1486	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	3.24e-180	1219	1466	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408201	Thioredoxin_14	2.14e-96	408	654	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	1.16e-62	1052	1163	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
408199	Thioredoxin_12	8.76e-59	35	219	1	177	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SMR54488.1|GT24	0.0	1	1542	1	1542
SMQ51945.1|GT24	0.0	1	1542	1	1542
SMY25588.1|GT24	0.0	1	1542	1	1542
SMR56403.1|GT24	0.0	1	1542	1	1542
UJO21924.1|GT24	0.0	8	1542	5	1547

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	21	1494	7	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	21	1494	7	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	24	1163	5	1124	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	21	1494	7	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	21	1494	7	1221	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	2.38e-264	12	1486	9	1424	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	1.72e-232	25	1492	30	1513	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	2.78e-218	24	1497	46	1534	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q6P5E4|UGGG1_MOUSE	3.82e-216	16	1483	39	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	2.11e-214	16	1483	39	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000235	0.999728	CS pos: 21-22. Pr: 0.9732

TMHMM  Annotations     

There is no transmembrane helices in ZTRI_6.565.mRNA-p1.