CAZyme Information: YALI0_E13926g-t26_1-p1

Basic Information

• Species: Yarrowia lipolytica
• Lineage: Ascomycota; Saccharomycetes; ; Dipodascaceae; Yarrowia; Yarrowia lipolytica
• CAZyme ID: YALI0_E13926g-t26_1-p1
• CAZy Family: GT76
• CAZyme Description: YALI0E13926p

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
390	CR382131|CGC1	43063.71	4.9150

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_YlipolyticaCLIB122	7223	284591	775	6448

• Gene Location: location=CR382131:1676053-1677225(-)

Full Sequence      

MGNAQSQEEANNNGAARQHRPADNNGHHDPKGTADPQPIPQEGSDNTQNHVSHSPEQMSA
NSSHVSATPFNNPFEPASYHKESPAPESVPVPVAPAKSHPQRPKMRRKSTLLLDEDEPVG
ENDDDIPFDKLNITHPTSNEPKPLSRVDSGEEIELTSPQVTEGKIPLEIKWTQGGSKVYV
TGTFTGWRKMVALTPDPNKKGVFSTTLHLPPGTHRLRFVVDNELRCSDYLPTATDSMGNL
LNYVEVGLSDTEERADQKDLHPISRAGIIPSNDDLGGGYERFTEEDLPKEEYEFTPEIPA
LFTDTEVMEQYISKELPTPPQLPPHLDSVILNTNSTEKEDNSVLPIPNHVILNHLATTSI
KHNVLAVASVSRYSRKYVTQVLYAPLPLQA

Enzyme Prediction     

No EC number prediction in YALI0_E13926g-t26_1-p1.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
214973	AMPKBI	9.03e-43	288	386	1	100	5'-AMP-activated protein kinase beta subunit, interation domain. This region is found in the beta subunit of the 5'-AMP-activated protein kinase complex, and its yeast homologues Sip1, Sip2 and Gal83, which are found in the SNF1 kinase complex. This region is sufficient for interaction of this subunit with the kinase complex, but is not solely responsible for the interaction, and the interaction partner is not known. The isoamylase N-terminal domain is sometimes found in proteins belonging to this family.
406865	AMPK1_CBM	1.15e-40	165	252	1	85	Glycogen recognition site of AMP-activated protein kinase. AMPK1_CBM is a family found in close association with AMPKBI pfam04739. The surface of AMPK1_CBM reveals a carbohydrate-binding pocket.
398422	AMPKBI	8.20e-34	317	385	1	69	5'-AMP-activated protein kinase beta subunit, interaction domain. This region is found in the beta subunit of the 5'-AMP-activated protein kinase complex, and its yeast homologs Sip1, Sip2 and Gal83, which are found in the SNF1 kinase complex. This region is sufficient for interaction of this subunit with the kinase complex, but is not solely responsible for the interaction, and the interaction partner is not known. The isoamylase N-terminal domain (pfam02922) is sometimes found in proteins belonging to this family.
199889	E_set_AMPKbeta_like_N	1.22e-26	166	246	1	80	N-terminal Early set domain, a glycogen binding domain, associated with the catalytic domain of AMP-activated protein kinase beta subunit. E or "early" set domains are associated with the catalytic domain of AMP-activated protein kinase beta subunit glycogen binding domain at the N-terminal end. AMPK is a metabolic stress sensing protein that senses AMP/ATP and has recently been found to act as a glycogen sensor as well. The protein functions as an alpha-beta-gamma heterotrimer. This N-terminal domain is the glycogen binding domain of the beta subunit. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include pullulanase, maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, and isoamylase.
199878	E_set	7.80e-08	171	222	7	58	Early set domain associated with the catalytic domain of sugar utilizing enzymes at either the N or C terminus. The E or "early" set domains of sugar utilizing enzymes are associated with different types of catalytic domains at either the N-terminal or C-terminal end. These domains may be related to the immunoglobulin and/or fibronectin type III superfamilies. Members of this family include alpha amylase, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase. A subset of these members were recently identified as members of the CBM48 (Carbohydrate Binding Module 48) family. Members of the CBM48 family include pullulanase, maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, isoamylase, and the beta subunit of AMP-activated protein kinase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAG79514.1|CBM48	3.90e-285	1	390	1	390
AOW05390.1|CBM48	1.59e-284	1	390	1	390
QNQ00168.1|CBM48	1.59e-284	1	390	1	390
ANB14517.1|CBM48	7.13e-103	101	386	168	474
AQZ09407.1|CBM48	9.08e-81	101	386	15	293

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2QLV_B	2.15e-74	165	386	4	252	Crystal structure of the heterotrimer core of the S. cerevisiae AMPK homolog SNF1 [Saccharomyces cerevisiae],2QLV_E Crystal structure of the heterotrimer core of the S. cerevisiae AMPK homolog SNF1 [Saccharomyces cerevisiae]
4QFG_B	7.09e-28	166	386	13	204	Structure of AMPK in complex with STAUROSPORINE inhibitor and in the absence of a synthetic activator [Rattus norvegicus],4QFR_B Structure of AMPK in complex with Cl-A769662 activator and STAUROSPORINE inhibitor [Rattus norvegicus],4QFS_B Structure of AMPK in complex with Br2-A769662core activator and STAUROSPORINE inhibitor [Rattus norvegicus],5KQ5_B AMPK bound to allosteric activator [Rattus norvegicus],5UFU_B Structure of AMPK bound to activator [Rattus norvegicus]
5T5T_B	9.85e-28	166	386	13	204	AMPK bound to allosteric activator [Rattus norvegicus]
3T4N_B	2.22e-27	293	386	2	110	Structure of the regulatory fragment of Saccharomyces cerevisiae AMPK in complex with ADP [Saccharomyces cerevisiae S288C],3TDH_B Structure of the regulatory fragment of sccharomyces cerevisiae AMPK in complex with AMP [Saccharomyces cerevisiae S288C],3TE5_B structure of the regulatory fragment of sacchromyces cerevisiae ampk in complex with NADH [Saccharomyces cerevisiae S288C]
6C9F_B	2.64e-27	166	386	13	204	AMP-activated protein kinase bound to pharmacological activator R734 [Homo sapiens],6C9G_B AMP-activated protein kinase bound to pharmacological activator R739 [Homo sapiens],6C9H_B non-phosphorylated AMP-activated protein kinase bound to pharmacological activator R734 [Homo sapiens],6C9J_B AMP-activated protein kinase bound to pharmacological activator R734 [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q04739|GAL83_YEAST	2.51e-76	166	386	163	416	SNF1 protein kinase subunit beta-3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GAL83 PE=1 SV=1
sp|P34164|SIP2_YEAST	1.39e-71	165	386	164	412	SNF1 protein kinase subunit beta-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SIP2 PE=1 SV=3
sp|P78789|AAKB_SCHPO	1.24e-42	96	383	28	293	5'-AMP-activated protein kinase subunit beta OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=amk2 PE=1 SV=2
sp|O43741|AAKB2_HUMAN	2.42e-26	119	386	37	272	5'-AMP-activated protein kinase subunit beta-2 OS=Homo sapiens OX=9606 GN=PRKAB2 PE=1 SV=1
sp|P80386|AAKB1_RAT	1.18e-25	166	386	79	270	5'-AMP-activated protein kinase subunit beta-1 OS=Rattus norvegicus OX=10116 GN=Prkab1 PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000042	0.000008

TMHMM  Annotations     

There is no transmembrane helices in YALI0_E13926g-t26_1-p1.