CAZyme Information: XP_003437253.1

Basic Information

• Species: Podospora anserina
• Lineage: Ascomycota; Sordariomycetes; ; Podosporaceae; Podospora; Podospora anserina
• CAZyme ID: XP_003437253.1
• CAZy Family: GT39
• CAZyme Description: Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Source:UniProtKB/TrEMBL;Acc:B2AMZ6]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
423	CU633872|CGC4	47665.28	9.9199

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PanserinaSmat	10888	515849	370	10518

• Gene Location: location=CU633872:122921-124317(+)

Full Sequence      

MTGRKPPLHQQALGLVGDILNGRHMLSQVIAPALFLADAVLCALVIRLIPYTEIDWKAYM
EQVSQFVSGERDYTKIRGGTGPLVYPAAHVYTYTGLYYLTDEGKNILLAQKLFAVLYVVT
LGVVMRCYRNARVSKRYIIQRLHSIFVLRCFNDCFAVLFLFLTILAFQNHSWRAGVLFYT
WGLGIKMSLLLVLPAVGIILLLGTGLNSALQSAAIIALVQVLIGVPFLANNPWGYLGRAF
ELSRQFFFKWTVNWRFMGEQAFLSKEFALVLLALHVLALLSFLTSRWLKGTGRSLSHIIT
SVLQVKSPFLPQEQEAVAEAITPEYVMTTILSANVIGLLFARSLHYQFYAYLAWSTPYLL
YKSRIHPVLQYMLWAAQEWAWNVYPSTPFSSGVVIGVMATTVASVWLGTKRSVLWVDNAE
KTK

Enzyme Prediction     

EC	2.4.1.258:10

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	32	391	9.4e-144	0.9945054945054945

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	0.0	32	391	1	358	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAP65337.1|GT58	7.90e-304	1	423	1	423
CDP31333.1|GT58	7.90e-304	1	423	1	423
VBB84570.1|GT58	4.45e-293	1	423	1	428
AEO60805.1|GT58	3.78e-201	1	411	15	430
AEO66311.1|GT58	3.90e-191	5	423	4	439

PDB Hits     

XP_003437253.1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9C1K8|ALG3_NEUCR	9.10e-187	6	411	12	424	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=alg-3 PE=3 SV=1
sp|Q2U6A4|ALG3_ASPOR	9.77e-135	20	412	12	398	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alg3 PE=3 SV=1
sp|A1CBE6|ALG3_ASPCL	3.26e-129	15	413	68	462	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=alg3 PE=3 SV=1
sp|Q4WVG2|ALG3_ASPFU	6.13e-127	20	421	20	414	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=alg3 PE=3 SV=1
sp|A1DDZ3|ALG3_NEOFI	7.03e-124	20	421	12	406	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=alg3 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000018	0.000020

TMHMM  Annotations     

Start	End
29	51
106	125
145	167
177	199
206	228
261	283
330	352
389	408