CAZyme Information: XP_001912340.1

Basic Information

• Species: Podospora anserina
• Lineage: Ascomycota; Sordariomycetes; ; Podosporaceae; Podospora; Podospora anserina
• CAZyme ID: XP_001912340.1
• CAZy Family: GT2
• CAZyme Description: Beta-mannosidase [Source:UniProtKB/TrEMBL;Acc:B2A9R0]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
854		96963.56	4.8293

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PanserinaSmat	10888	515849	370	10518

• Gene Location: location=CU633438:497095-499715(+)

Full Sequence      

MAPAAVIVVPVDKNWEFKQLDKEDAEFLPVSQFPTNVHLDLIHHKIIPDPFIGKNELDVQ
WVGEAQWVYKTTFKGQPVPEGAKAVLAFDGLDTFATVKVNGTTILETDNMFNPERVEVTG
LLKEENELVISFDSAYLRGWKLVEKYPEHKWGVWNGDNSRLAVRKAQYHWGWDWGPALLT
CGPWRPINLEIYETRLADLSFDSTISENLKSAGIKATAEVEGPGGKVKFELSLDGEQVAA
QTADANGSGSVTVSFHLDNPSLWYPFRYGEQPLYTLTATILSGDDEISSISKRIGLRRAE
LVQQPLLDQPGTSFFFRVNNIPIYCGGSDWIPADNFIPRITAQKYYEWISLIRDGNQFMV
RVWGGGIYEEQAFYDACDELGVLVWQDFMFGCGNYPAWPELRKSIDREARENVKLLRHHP
SIVIWAGNNEDYQYQESAGLTYDFENKDAESWLQTDFPARYIYEKILSDACAELIPSTFY
HPGSPWGAGRDTHDATVGDIHQWNVWHGTQEKWQDFDKLSGRFVSEFGMQAFPDVKTIDA
YLPLGKDDPDRYPQSSTVDFHNKADGHERRIALYLVENMRYAPDPLEQFVYSTQLMQAEC
LASAYRLWKRQWKGPGREYNAGALVWQINDCWPVTSWSIVDFYLRPKHAFYTVKREMAPV
SAGITRKIHKHPRDKYTRVYIDTETKIEVWGSNLTLEDLTVDVVLKAWDVTTGELTLEKK
IAEGLVLPENRSTEVGALDVPVREKGKGEENKIVVTAYIVQNGKQIARYVNWPEPLKYVH
LQKPKELKVVLNDDATAVDISAEVPVKGVALECEDDAVKFDDNLVDIVPGEVVSIAVKGA
TKETKISTRYLGMI

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	10	756	3.5e-91	0.7101063829787234

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	8.41e-82	10	654	14	627	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	8.10e-16	685	778	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
395572	Glyco_hydro_2	2.32e-12	194	297	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
236657	PRK10150	1.62e-08	60	438	62	425	beta-D-glucuronidase; Provisional
236548	lacZ	4.53e-06	193	307	220	351	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAP59820.1|GH2	0.0	1	854	1	854
CDP22463.1|GH2	0.0	1	854	1	854
VBB71464.1|GH2	0.0	1	854	1	854
AEO69937.1|GH2	0.0	1	854	1	851
EAA35570.1|GH2	0.0	1	854	1	854

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	2.48e-144	4	838	22	811	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	7.23e-121	8	831	8	810	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	7.59e-121	8	831	10	812	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	7.77e-121	8	831	10	812	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	2.03e-120	8	831	8	810	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|I2C092|MANBB_THETO	0.0	1	854	1	855	Beta-mannosidase B OS=Thermothelomyces thermophilus OX=78579 GN=man9 PE=1 SV=1
sp|A1CGA8|MANBB_ASPCL	1.70e-300	4	852	2	844	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
sp|A1D911|MANBB_NEOFI	1.68e-295	10	852	8	844	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
sp|Q0CCA0|MANBB_ASPTN	1.79e-294	14	852	12	842	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
sp|Q4WAH4|MANBB_ASPFU	4.42e-293	10	852	8	844	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000061	0.000000

TMHMM  Annotations     

There is no transmembrane helices in XP_001912340.1.