CAZyme Information: VDB92423.1

Basic Information

• Species: Blumeria graminis
• Lineage: Ascomycota; Leotiomycetes; ; Erysiphaceae; Blumeria; Blumeria graminis
• CAZyme ID: VDB92423.1
• CAZy Family: GT21
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
904	LR026992|CGC9	102574.31	7.1838

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_BgraminisTritici96224	8470	1268274	123	8347

• Gene Location: location=LR026992:205056-207944(+)

Full Sequence      

MTVFVGSLFLPDTIHFAIPSQVTEDDPYTTQRRSIGGNNQNLSLPSFPSVRDPTPPQTPT
VVKNHDDDKFFARSDNPELRGQVSSSAKLAPNNPNSRAWGAGTVFNQPKSRASSPPPANI
LRHNRTLRKAKSFGRPGNRHPHLGRHENQDKLFASAGWVVVPADQGNGGLRNAVQAAVRD
GKLEDKMWVGTLGMPTDVLEGTQQKEEIERRLAVEYDSLTVFCKDSDFNGHYTHYSKQIL
WPVLHYQIPDNPKSKAYEDHSWVYYVRVNQTFADKIIENWKKGDVIWIHDYHLLLVPRMI
RQKLPDAKIGFFLHVAFPSSEVFRCLAVRTELLEGMLGANLIGFQIYEYARHFLQTCSRL
LCVEATKEGVQLEDRFVDVISLAIGIDPIALDANRADPDVSKWIKTMQKRYQGKKLIVAR
DKLDHVRGVRPKLLAYELFLNKYPEWRNNVVMIQVATSTTEQAELDATVSDIVTRVNSSW
ANLAYQPLVYLKQDISYAQYIALLTIADALMITSQREGMNLTCHEFLFCQDGKFEGQNKN
GCLILSEFTGSASVFGRNELSVNPWDYRQCADAIKTALEMSEDERKLRWNNLYKAVIHHT
AEYWFSSFLTRLDEVYDEQNLRCTTSISRLSIDKLGQDYRSASRRLFILDYEGTLASWGS
PNDIILTSPQRTIDILNDISLDVRNIVYVMSARQPEELDRLFKRVPNLGLVAENGCYIKL
SNTSKWIENADPAEMREWKESVKGIMRYYHERTPGSWIETRHCSLVFHYRSCEDQESAGR
LAGDCASHINDACEGQRVRAVLVEGTVVVEPIEWSKSTAAVTIFGHLGNNFADQDSKTID
FLMVVGNNRDDEVIFRWANELGRLKKVRNVTTVSLGSRTTEANYTLTQGVTGVLSVLQKI
STIL

Enzyme Prediction     

EC	2.4.1.15:11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT20	161	613	1e-159	0.9284210526315789

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340820	GT20_TPS	0.0	160	612	20	462	trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
395781	Glyco_transf_20	0.0	137	612	7	468	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.
184712	PRK14501	2.64e-174	157	903	20	726	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
223457	OtsA	1.23e-166	166	612	42	477	Trehalose-6-phosphate synthase [Carbohydrate transport and metabolism].
215555	PLN03063	1.50e-133	168	902	39	785	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VDB92423.1|GT20	0.0	1	904	1	904
ABW72067.1|GT20	0.0	1	904	1	904
QSZ35222.1|GT20	0.0	1	901	1	923
ATZ46284.1|GT20	0.0	1	901	1	920
APA06495.1|GT20	0.0	1	901	1	919

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JBI_A	7.03e-99	208	615	61	465	Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBI_B Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBR_A Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_B Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_D Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_F Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_H Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_K Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_M Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_O Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBW_A Structure of Tps1/UDP complex [Pyricularia oryzae 70-15],6JBW_B Structure of Tps1/UDP complex [Pyricularia oryzae 70-15]
5DXF_A	1.06e-96	230	609	146	520	Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314],5DXF_B Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314]
5HVM_A	1.21e-96	208	615	73	477	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVM_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HVO_A	2.43e-94	167	612	38	474	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_C Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_D Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HUT_A	2.44e-93	167	622	31	477	Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUT_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUU_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HUU_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HVL_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314],5HVL_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O14081|TPSX_SCHPO	1.42e-208	55	866	97	904	Putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 106 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC2E11.16c PE=1 SV=1
sp|Q9UUI7|TPSY_SCHPO	2.45e-204	4	900	5	889	Putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 100 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC22F8.05 PE=1 SV=1
sp|P38426|TPS3_YEAST	3.77e-169	148	849	304	982	Trehalose synthase complex regulatory subunit TPS3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPS3 PE=1 SV=3
sp|P38427|TSL1_YEAST	2.02e-165	148	819	336	983	Trehalose synthase complex regulatory subunit TSL1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TSL1 PE=1 SV=1
sp|Q54K57|TPSA_DICDI	1.58e-129	177	773	48	628	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A OS=Dictyostelium discoideum OX=44689 GN=tpsA PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.900728	0.099303

TMHMM  Annotations     

There is no transmembrane helices in VDB92423.1.