CAZyme Information: VBB72720.1

Basic Information

• Species: Podospora comata
• Lineage: Ascomycota; Sordariomycetes; ; Podosporaceae; Podospora; Podospora comata
• CAZyme ID: VBB72720.1
• CAZy Family: AA3
• CAZyme Description: Putative Glycosyltransferase Family 39

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
772	LR026964|CGC47	88122.34	9.5051

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PcomataTmat	13100	N/A	2652	10448

• Gene Location: location=LR026964:4288235-4290669(+)

Full Sequence      

MATTAPQGTLRQRSVAGSKNKDGASSDVELDKLVKAANQRPKAVSENDHRIAFFIITILA
FITRFWGISHPNEVVFDEVHFGKFASYYLEKTYFFDVHPPFGKLLFAFVGWLVGFDGHFH
FENIGDSYIANKVPYVAFRSLPALLGALTVSVVYLIMWESGYSLPACILAAGLVLLDNAH
IGQTRLILLDATLVFAMACSLLCYIKFYKLRHEPFSRKWWKWLILTGFALSCDISTKYVG
LFAFITIGSAVVIELWDLLDIKRPGGALTLAQFGKHFAARAFGLIFLPFLFYLFWFQVHF
TILTRSGPGDDFMTPEFQETLSDNIMLSNAITIDYYDTISIKHKETKAYLHSHPERYPLR
YDDGRVSSQGQQVTGYPFNDTNNYWQILPAGADDKQLNRHIKNHDLIRLRHVVTDTILLS
HDVASPYFPTNQEFTTVSLADAYGDRAADTLFEVRIEHGKPNQEWKTISGHFKLIHNPSK
VAMWTHTKPLPEWAFKQQEINGNKQIAPSSNVWLVEDISSLPANHPRRAKVAKKVKTLPF
LRKWFELQRSMFWHNNQLTASHPYASLPYSWPFLLRGVSFWTQNDTRQQIYFLGNPIGWW
IASSVLAIYAGIILADQFSLRRGMDALDHRSRSRLYNSTGFFFLAWATHYFPFYVMGRQL
FLHHYLPAHLASALVTGALVEFIFSQDAVEHEVAHQAAKAGKKSQTPKHHLTARERFAGQ
SLFGSWVATGVILTLVAAGWYFFLPLTYGYPGLTVEQVIRRKWLGYDLHFAK

Enzyme Prediction     

EC	2.4.1.109:8

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	55	300	5.3e-72	0.9955156950672646

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224839	PMT1	0.0	55	772	29	699	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
396786	PMT	8.28e-84	55	304	2	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
406576	PMT_4TMC	6.89e-77	542	764	1	197	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
397103	MIR	1.75e-15	351	500	13	170	MIR domain. The MIR (protein mannosyltransferase, IP3R and RyR) domain is a domain that may have a ligand transferase function.
224720	ArnT	5.22e-08	139	271	87	212	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VBB72720.1|GT39	0.0	1	772	1	772
CDP23606.1|GT39	0.0	1	772	1	773
CAP69590.1|GT39	0.0	1	772	1	773
AEO65833.1|GT39	0.0	2	772	3	777
AEO54171.1|GT39	0.0	2	772	3	775

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6P25_A	1.83e-105	56	767	56	728	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_A Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]
6P25_B	7.70e-90	7	668	20	681	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_B Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q59X23|PMT4_CANAL	1.97e-252	9	772	4	755	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PMT4 PE=2 SV=2
sp|O42933|PMT4_SCHPO	7.33e-248	42	764	51	767	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ogm4 PE=3 SV=1
sp|P46971|PMT4_YEAST	8.28e-243	46	772	47	762	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PMT4 PE=1 SV=1
sp|Q99PR0|POMT1_RAT	6.39e-113	39	668	28	669	Protein O-mannosyl-transferase 1 OS=Rattus norvegicus OX=10116 GN=Pomt1 PE=2 SV=1
sp|Q8R2R1|POMT1_MOUSE	3.37e-112	39	668	28	669	Protein O-mannosyl-transferase 1 OS=Mus musculus OX=10090 GN=Pomt1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999906	0.000094

TMHMM  Annotations     

Start	End
51	68
93	115
136	158
186	208
238	257
277	296
591	613
633	655
722	744