CAZyme Information: TSTA_084260-t26_1-p1

Basic Information

• Species: Talaromyces stipitatus
• Lineage: Ascomycota; Eurotiomycetes; ; Trichocomaceae; Talaromyces; Talaromyces stipitatus
• CAZyme ID: TSTA_084260-t26_1-p1
• CAZy Family: GH43
• CAZyme Description: beta-mannosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
926		103361.45	4.9700

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TstipitatusATCC10500	12629	441959	146	12483

• Gene Location: location=EQ962653:1050960-1054002(+)

Full Sequence      

MLMKILLGSGLLLPTFAQHVIDLTGNDWTVKNAEGNVSAPASLPSQVHLDLFAAGAIHTL
AQMKSIFGGSPGLTGHIPVRQSRACIIDGASSSWLVFNGLDTFATIELCNQTVGTANNQF
RQWKFDVSHALKSCKGHPVISISFSSAPKIANAIANEPGQETWPAGVQGVFEFENRWFIR
KEQSDFGWDWGPAFSPTGPWQPAYLVQFKPDNGVYVLNTDIDIHREGQVNWLPPDQSKPW
VVNASIDYLGTLPKGVSLTVEIKDSQNKTTIASGSLTNLTISGNSISGITTVGGDVPKLW
WPTNMGEQNLYYITINVVDGSNKTLATVTKRTGFRTIVLNQTNITDAQIALGIAPGANWH
FEINGHEFYAKGSNFIPPDAFWPRVTEDRMKRLFDSIVKGNQNMLRVWASGAYLPEFIYD
IADEKGVLLWSEFEFSDALYPVNQAFLDNVVEEVTYNVRRVNHHPSLALWSGGNELESLE
LQEVYQNDPAEYPRYVGEYEKLFISLIFPLVYDNSRSISYTPTSTGNGYTKIDFSLPVPM
VERYQNVTPGEYYGDSDYYNYISAEAFDMSHYPVDRFANEFGFHSMPSLQTWRQAVSDED
LIFNSSVILHRNHHYPPGGLSTDTTRSAAGMGEMTIAVELYYPIPLKLDPIVNFSAWCLA
TQRFQADMYKSEIQFYRRGSGRPERQLGSLYWQLEDIWQAPTWAGIEYGGRWKVLHYVAK
NIYDPVIVAPYWDSSTGDLNVTVTSDLWESVSGIVNMTWYDFKGKPLEKNAGVPSSVAFD
VGPLNITDVLTTNIKNLSIPTLKDAVLVLSLSATGRLPNSNTKAQFSHENYFTPVFMNEA
QLVDPELKLSYNKSTGKFTVEATNGVSLYTWLDYPAGIVGYFDKNSFVLVPGHPKEVGFT
VQEGSLTEKVVKEITVQSIWDQAKKD

Enzyme Prediction     

EC	3.2.1.25:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	92	795	2.1e-74	0.613031914893617

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.37e-46	13	730	5	636	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407625	Ig_mannosidase	5.64e-15	844	921	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
236673	ebgA	4.97e-10	204	475	202	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
395572	Glyco_hydro_2	1.07e-07	235	335	13	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
407658	Mannosidase_ig	4.43e-07	737	834	1	88	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGA14982.1|GH2	0.0	4	924	6	932
QKX59399.1|GH2	0.0	1	926	2	925
QMW39414.1|GH2	0.0	15	922	18	927
QMW27343.1|GH2	0.0	15	922	18	927
BAE57065.1|GH2	0.0	15	922	18	910

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CVU_A	0.0	15	921	25	938	Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum [Trichoderma harzianum],4UOJ_A Chain A, BETA-MANNOSIDASE GH2 [Trichoderma harzianum],4UOJ_B Chain B, BETA-MANNOSIDASE GH2 [Trichoderma harzianum]
6DDT_A	1.92e-101	28	890	18	836	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
2VJX_A	6.59e-68	95	775	84	702	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	6.77e-68	95	775	86	704	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	6.87e-68	95	775	86	704	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WMS9|MANBA_ASPFU	0.0	18	926	22	926	Beta-mannosidase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndA PE=3 SV=1
sp|O74168|MANBA_ASPAC	0.0	17	921	23	932	Beta-mannosidase A OS=Aspergillus aculeatus OX=5053 GN=mndA PE=1 SV=1
sp|Q2UN00|MANBA_ASPOR	0.0	15	922	18	910	Beta-mannosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndA PE=3 SV=1
sp|Q0CI48|MANBA_ASPTN	0.0	7	925	12	931	Beta-mannosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndA PE=3 SV=2
sp|Q5BCI8|MANBA_EMENI	0.0	13	922	16	924	Beta-mannosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000287	0.999717	CS pos: 17-18. Pr: 0.9703

TMHMM  Annotations     

There is no transmembrane helices in TSTA_084260-t26_1-p1.