CAZyme Information: TSTA_066940-t26_1-p1

Basic Information

• Species: Talaromyces stipitatus
• Lineage: Ascomycota; Eurotiomycetes; ; Trichocomaceae; Talaromyces; Talaromyces stipitatus
• CAZyme ID: TSTA_066940-t26_1-p1
• CAZy Family: GH3
• CAZyme Description: alpha-1,3-mannosyltransferase (Alg3), putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
415	EQ962652|CGC2	46412.66	8.5373

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TstipitatusATCC10500	12629	441959	146	12483

• Gene Location: location=EQ962652:2065676-2067029(+)

Full Sequence      

MTISNTIVDILTNPKHSLWIAPLLVVADAVLSALIIKKVPYTEIDWTTYMQQIALYQAGE
RDYTAIKGDTGPLVYPAAHVYIYSFLYELTNKGQDIVLGQVIFAGIYITTLVVVLSCYIR
AGAPPYLLPLLVLSKRLHSVYMLRLFNDGIATFAMWLGIFLFQRRQLTAATTVWSLGVGV
KMSLLLLAPGVAIVVALSGGIWTAVPLALNAILTQVLLGIPFLRENAIGYASRAFELTRQ
FLYEWTVNWRFVGEEVFESPIFSINLLVLHVVLILAFLATRWIRPSGEELDFFAQKFIEG
RSTRHPLSSKYITTTLLTSMVIGMLCARSLHYQFYAYLGWTTPFLLWQAKLHPVLIYAVW
AMQEWGWNVFPSTNASSAAVVACLAVQVVMLLLNDDSSQGLSEDIKKSVGEQKTK

Enzyme Prediction     

EC	2.4.1.258:10

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	21	377	8.6e-144	0.9972527472527473

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	0.0	22	377	1	358	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGA13922.1|GT58	5.25e-252	1	415	1	414
QKX58543.1|GT58	1.98e-203	7	394	152	539
BCR98022.1|GT58	3.87e-174	9	398	9	398
BCS10476.1|GT58	3.87e-174	9	398	9	398
BAE62911.1|GT58	3.15e-173	1	412	1	410

PDB Hits     

TSTA_066940-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2U6A4|ALG3_ASPOR	5.60e-174	1	412	1	410	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alg3 PE=3 SV=1
sp|Q4WVG2|ALG3_ASPFU	9.23e-172	1	407	9	415	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=alg3 PE=3 SV=1
sp|A1DDZ3|ALG3_NEOFI	2.55e-162	1	407	1	407	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=alg3 PE=3 SV=1
sp|A1CBE6|ALG3_ASPCL	1.46e-161	1	399	63	461	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=alg3 PE=3 SV=1
sp|A2RA94|ALG3_ASPNC	1.40e-146	9	398	9	394	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=alg3 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000047	0.000002

TMHMM  Annotations     

Start	End
17	36
72	89
96	118
140	162
174	196
201	223
261	283
311	330