CAZyme Information: TSTA_047570-t26_1-p1

Basic Information

• Species: Talaromyces stipitatus
• Lineage: Ascomycota; Eurotiomycetes; ; Trichocomaceae; Talaromyces; Talaromyces stipitatus
• CAZyme ID: TSTA_047570-t26_1-p1
• CAZy Family: GH15|CBM20
• CAZyme Description: beta-galactosidase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
977	EQ962657|CGC16	108054.57	4.8520

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TstipitatusATCC10500	12629	441959	146	12483

• Gene Location: location=EQ962657:1937249-1940336(+)

Full Sequence      

MAASSSSSPVTWDKYSLSINGERLFVFAGEFHYLRLPVPELWLDVFQKLKANGFNAISVY
FFWNYHSASEGIYDFESGAHNIQRLFDYAKETGIYIIARPGPYANGELSAGGFALWAANG
LLGRERTSDSQYYNLWSPWMTEIGKIIAANQITEGGPVILVQHENELQETTHSANNTLVL
YMEQILKVLADAGIVVPSTHNEKGMRSVSWSTDYENVGGAVNIYGLDSYPGGLSCTNPNA
GFDLIRTYYQWFQNYSYTQPEFLPEFEGGYFTPWGDVFYDDCSSMLLPEYADVFYKNNIG
SRITLQSLYMTYGGTNWGHIAAPVVYTSYDYNAPLRETREIRDKLKQTKLLGLFTRVSSD
LLQTDMMGNGTGYTTGADIFTWALRNPETNAGFYVVAQEDSSSVTDVVFDLEVETSTGPV
KISNIELSGRQSKIIATDYKVGDTTLLYCSTDILTYATLDVDVLVLYLNEGQTGTFVLAR
SSSGLKYIVYGNSTVTTSQSSHGTVYTYTQGKGVSAIKFSNGYLVYLLDKYTAWNFFAPS
LVSSPIVKPDEHLFVIGPYLVREASIKDHTLELRGDNENTTSIEIYHGNPSISTITWNNK
PLPTTRTAYGSLIGTIPGTEHISISLPKLTSWKSHNTIPEIDPNYDDSNWVVCDKKTTFN
SIPPMSFPVLYSGDYGYHAGPKIYRGRFGNTNATGVNITAQNGYAAGWSAWLNGKYVGGE
PGDPSMEATSAVLKFDSSNLKQDDTENVLTVLVDYTGHDEDNVRPTGAQNPRGLLGAVFQ
GPTSTDFTSWKIQGNAGGEKNIDPLRGPMNEGGLYGERLGWHLPGFKPSTESGWDDKSPS
DGVHGASGRFYLTEFTFDLGANSHTLDVPIGIRLNASSTSGPAVVYVWLNGYKFAHYLPH
IGPQTVFPFQPGILNIQGSDGRERTNTLAICIWAMTGQSVALDVVELVVYGKYVSSFNFG
RDWRYLQPGWKDRSEYA

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH35	17	351	1.1e-81	0.9869706840390879

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396048	Glyco_hydro_35	1.40e-82	16	352	1	315	Glycosyl hydrolases family 35.
402180	BetaGal_dom2	1.23e-66	361	535	1	180	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyzes the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C-terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N-terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, pfam01301, which is N-terminal to it, but itself has no metazoan members.
198097	BetaGal_dom2	6.59e-54	360	536	1	182	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyses the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, which is N-terminal to it, but itself has no metazoan members.
404274	BetaGal_dom4_5	2.10e-30	815	938	1	111	Beta-galactosidase jelly roll domain. This domain is found in beta galactosidase enzymes. It has a jelly roll fold.
166698	PLN03059	3.84e-29	4	350	24	344	beta-galactosidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGA19491.1|GH35	0.0	3	977	23	999
QKX62644.1|GH35	0.0	3	977	20	985
BAE57280.1|GH35	0.0	8	977	30	984
QMW27553.1|GH35	0.0	8	977	30	984
QRD81954.1|GH35	0.0	8	977	30	984

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1TG7_A	2.39e-170	10	948	6	944	Native structure of beta-galactosidase from Penicillium sp. [Penicillium sp.],1XC6_A Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose [Penicillium sp.]
5IFP_A	2.05e-169	10	947	46	979	Structure Of Beta-galactosidase From Aspergillus Niger [Aspergillus niger CBS 513.88]
5IFT_A	5.68e-169	10	947	46	979	STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 3-b-Galactopyranosyl glucose [Aspergillus niger CBS 513.88],5IHR_A Structure Of E298q-beta-galactosidase From Aspergillus Niger In Complex With Allolactose [Aspergillus niger CBS 513.88],5JUV_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-b-Galactopyranosyl galactose [Aspergillus niger CBS 513.88],5MGC_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 4-Galactosyl-lactose [Aspergillus niger CBS 513.88],5MGD_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose [Aspergillus niger CBS 513.88]
3OG2_A	2.45e-163	10	947	26	975	Chain A, Beta-galactosidase [Trichoderma reesei],3OGR_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGS_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGV_A Chain A, Beta-galactosidase [Trichoderma reesei]
4IUG_A	6.19e-155	10	947	46	977	Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose [Aspergillus oryzae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A7EZS5|BGALC_SCLS1	0.0	10	977	28	984	Probable beta-galactosidase C OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) OX=665079 GN=lacC PE=3 SV=1
sp|B6H5X9|BGALC_PENRW	0.0	10	977	30	982	Probable beta-galactosidase C OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=lacC PE=3 SV=1
sp|A1CE56|BGALC_ASPCL	0.0	10	977	32	985	Probable beta-galactosidase C OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=lacC PE=3 SV=1
sp|A2QL84|BGALC_ASPNC	0.0	10	977	28	994	Probable beta-galactosidase C OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=lacC PE=3 SV=1
sp|Q4WNE4|BGALC_ASPFU	0.0	10	977	32	983	Probable beta-galactosidase C OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=lacC PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000084	0.000000

TMHMM  Annotations     

There is no transmembrane helices in TSTA_047570-t26_1-p1.