CAZyme Information: TSTA_025220-t26_1-p1

Basic Information

• Species: Talaromyces stipitatus
• Lineage: Ascomycota; Eurotiomycetes; ; Trichocomaceae; Talaromyces; Talaromyces stipitatus
• CAZyme ID: TSTA_025220-t26_1-p1
• CAZy Family: AA8
• CAZyme Description: beta-N-acetylglucosaminidase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2237	EQ962654|CGC9	246434.75	6.9075

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TstipitatusATCC10500	12629	441959	146	12483

• Gene Location: location=EQ962654:538319-546836(+)

Full Sequence      

MASDFSILTPNPMLGYGYKLEHFWYGIEKYSPKAIIVDSGSTDGGPYKLGLNKMTCGRDS
YIRDLTPMLQACYYKKIKVLISSVGGDGSDKHVQEMLEIVREISKKHGFSFKVATISAGF
DKGLVKDRILKSKVSPCGPVEELTVDSVERTIDIVGQMGAEPYLKALEMDPDIVLGGRSY
DPAPFAAFSMFHRVEPATAWHMGKIMECGGICAVPKGRSMVVTMRKYSFDLTPLSPKERC
TPTSVAAHTLYEKSRPDRLPGPGGALILDDASYEQITEKSIRVRGARFEPTPIYQVKLEG
VEKLGYRTIFVGGVRDPILISQIDEFLAEVRAYTQNLFPQLDQTPQCRLIFHFYGRNGVM
GPLEPDPKSAHELGVMGEVVAPSQEMSYTIANNVRASILHMPYKNQVATTGNFASPLSPH
ETPAGPVFRFNVYHLVDLEKGEEETLFPIRFVTIENPPPPGSESVPRLTTEQREQLASET
LEPLVFKQIPEGECQMLDIAKIIRSKNSGPFELTLDIMFDSNDAYERVKNANILTNERIQ
TLYHLKPGDILTNTFFDPALAWKCTIRRPWEQGTVGERDTLGTQQHAPLLTIRVPKPKNS
TAASPKPQTNVQYFQDRSHFSTRDSVEYIWRGLGLPEYSLKALQLTGDGLGLPSSFKIGH
IAQASIGLSALLAALIYGHRKQIPTPTVTIPLQHASIEFKSERLYRLTNKPTPSPWGPIG
GLHKTADGYVRVHDSFPNHRDGARKLIGCSPGASREEVATAIAPWRSIDLEWAGFDSNIV
ISALRSYSEWDVLPQAKAIADFPILLRKLSDGPVGLPQEMTDVDPDKCLCGLRVLELSRV
IAAPLSGRTLAAHGADVLWVTSPDLPDLPAIDRDFGRGKRTIQLDLNDEADTNEFHRLLE
NAHVFTQGFRPGSLARKGLSSAALAARFKDRNIICANMSAYGPDGPWSNKRGFDSIVQTC
SGMNVSEAEHYGAGEPARATCCQSLDHAGGYFLAAGIMAALYKQATEGGSWEVNVSLAGA
MKYLRSLGQYEGYLFAVMNFSNNVSFASLPLFLPTIVSEMGEFTAVESNGLLAPPYFIYF
PLIIAISVISDRVRLRGPFPVFFALLSALRFILLGATTSVISRYIGTFLAVLIFVTTSIV
LIWNANTNSTSSKRAGGLWIIMTVGQCGPLLGTNVFPSSQAPLYRERSWICCTFALLFAV
TVMMLSFSLWQENKKMDRLYGPLEESGPVERSENDGIPRYRYIIEAFWQPRVYLIIQSAA
QNTDTSNKKKARVIAICIRHPGTNPLSKLSRLHAGRTHIIPSSPLGPHDDCYRYDGQLSA
KRYTISHFGGVSVLFHVLTAITRLVILSDLHITRVKDHQALAMASSADPALRKKVGQLFA
VGFHGFTPSPEIKTLIRDYGLGAVILFRRNIKDAVQLKTLTLALQQEAKEAGHEHPLFIG
IDQENGLVTRIMPPLAVQQPGQMTIGATQSAENAYEVGKCTGELLSFFGINMNYAPDCDI
NSEPLNPVIGVRSAGDDPNFVARFSCATAKGLRESGVVPTAKHFPGHGDTAVDSHHGLPV
ISKSRSELERCELIPFRRAVAEGIETVMTAHIALPKVTTGPDSEGLPATLSPEALGILRN
DMKYDGVIVTDCLEMDGIRATYGTVEGSLMSLIAGSDSIMICHTYAVQVKSIERVVQAVQ
LGALSESRIDESLHRIGKLKQRFLSWEDVLRSVDNNSVGLSSMNQRHEQIANNVYSQSTT
VVRNSLGVVPISKSISKVLLLAPGGKIPAGGAVDDSGPRHRTYIDVLRDVAGTASSVMQL
DYPDTGLLSDEQWKTIETEADVVILTTRNAKEAKGQKEFALELARRRKDVIVIATCSPYD
FLDDEEIIKTYIAIYEPTAEAFTSAIDVIFGRASATGVLPVASNPHTKTNKSYKILALDE
SRKDEMITGILDVWNKALPDYTLPAGNLRKVIDQPNGHHFIAHDAQTGRIIGFCLLYTSQ
NQGQTSAEIAVIAVDPVKQRQGIGSELLSETREYLYEYHGVKTASLRSYFPRFWPGLPAD
LSPEDEQFFIHRGFQFTRSGDADLYQDIRKFVPPEQYLERAAASNVTYKAIETPDEFENC
LVGQKRNFSHYTGWVETYIALHPSKHPSSIMAAFDKDGTQIAWTLMLSPDDDFVAQNWAF
SPLASGNQQPLKTGIIGCVGVDESHRSRGVGLALLCYAIESMKKRGIEAAFVDSTDIVGW
YGKVGFGKWKEYIHAEI

Enzyme Prediction     

EC	3.2.1.52:8

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	1433	1660	4.4e-52	0.9629629629629629

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224389	BglX	1.68e-83	1371	1779	3	378	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
395747	Glyco_hydro_3	1.34e-76	1371	1696	2	313	Glycosyl hydrolase family 3 N terminal domain.
405080	DUF4387	2.62e-50	496	593	1	98	Domain of unknown function (DUF4387). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria, archaea and eukaryotes. Proteins in this family are approximately 110 amino acids in length. There is a conserved RSKN sequence motif.
235417	PRK05337	4.10e-43	1385	1712	10	319	beta-hexosaminidase; Provisional
396872	CoA_transf_3	1.16e-41	829	1021	1	200	CoA-transferase family III. CoA-transferases are found in organisms from all lines of descent. Most of these enzymes belong to two well-known enzyme families, but recent work on unusual biochemical pathways of anaerobic bacteria has revealed the existence of a third family of CoA-transferases. The members of this enzyme family differ in sequence and reaction mechanism from CoA-transferases of the other families. Currently known enzymes of the new family are a formyl-CoA: oxalate CoA-transferase, a succinyl-CoA: (R)-benzylsuccinate CoA-transferase, an (E)-cinnamoyl-CoA: (R)-phenyllactate CoA-transferase, and a butyrobetainyl-CoA: (R)-carnitine CoA-transferase. In addition, a large number of proteins of unknown or differently annotated function from Bacteria, Archaea and Eukarya apparently belong to this enzyme family. Properties and reaction mechanisms of the CoA-transferases of family III are described and compared to those of the previously known CoA-transferases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGA12539.1|GH3	0.0	1363	2237	1	877
QKX56918.1|GH3	0.0	1371	2237	7	854
BCR85716.1|GH3	4.55e-314	1371	2232	6	849
CAP94355.1|GH3	1.22e-304	1371	2237	6	852
QQK41162.1|GH3	1.66e-300	1363	2237	1	852

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZM6_A	2.61e-129	1363	2232	1	853	A unique GCN5-related glucosamine N-acetyltransferase region exist in the fungal multi-domain GH3 beta-N-acetylglucosaminidase [Rhizomucor miehei CAU432],4ZM6_B A unique GCN5-related glucosamine N-acetyltransferase region exist in the fungal multi-domain GH3 beta-N-acetylglucosaminidase [Rhizomucor miehei CAU432]
6K5J_A	8.57e-91	1371	1900	13	528	Structure of a glycoside hydrolase family 3 beta-N-acetylglucosaminidase from Paenibacillus sp. str. FPU-7 [Paenibacillaceae]
3BMX_A	4.40e-60	1370	1901	43	615	Beta-N-hexosaminidase (YbbD) from Bacillus subtilis [Bacillus subtilis],3BMX_B Beta-N-hexosaminidase (YbbD) from Bacillus subtilis [Bacillus subtilis],3NVD_A Structure of YBBD in complex with pugnac [Bacillus subtilis],3NVD_B Structure of YBBD in complex with pugnac [Bacillus subtilis]
3LK6_A	1.18e-59	1370	1901	17	589	Chain A, Lipoprotein ybbD [Bacillus subtilis],3LK6_B Chain B, Lipoprotein ybbD [Bacillus subtilis],3LK6_C Chain C, Lipoprotein ybbD [Bacillus subtilis],3LK6_D Chain D, Lipoprotein ybbD [Bacillus subtilis]
4GYJ_A	2.19e-59	1370	1901	47	619	Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYJ_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYK_A Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168],4GYK_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P40406|NAGZ_BACSU	2.27e-59	1370	1901	43	615	Beta-hexosaminidase OS=Bacillus subtilis (strain 168) OX=224308 GN=nagZ PE=1 SV=1
sp|P48823|HEXA_PSEO7	1.70e-56	1387	1763	50	437	Beta-hexosaminidase A OS=Pseudoalteromonas piscicida OX=43662 GN=cht60 PE=1 SV=1
sp|A9M1Z4|NAGZ_NEIM0	1.94e-36	1386	1663	18	290	Beta-hexosaminidase OS=Neisseria meningitidis serogroup C (strain 053442) OX=374833 GN=nagZ PE=3 SV=1
sp|Q9K0Q4|NAGZ_NEIMB	2.63e-36	1386	1663	18	290	Beta-hexosaminidase OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=nagZ PE=3 SV=1
sp|A1KSD9|NAGZ_NEIMF	2.19e-35	1386	1663	18	290	Beta-hexosaminidase OS=Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18) OX=272831 GN=nagZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000052	0.000000

TMHMM  Annotations     

Start	End
1071	1090
1097	1114
1124	1143
1156	1178
1188	1210
1325	1347