CAZyme Information: TSTA_016530-t26_1-p1

Basic Information

• Species: Talaromyces stipitatus
• Lineage: Ascomycota; Eurotiomycetes; ; Trichocomaceae; Talaromyces; Talaromyces stipitatus
• CAZyme ID: TSTA_016530-t26_1-p1
• CAZy Family: AA4
• CAZyme Description: cell wall glucanase (Utr2), putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
440		46563.08	4.1452

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TstipitatusATCC10500	12629	441959	146	12483

• Gene Location: location=EQ962656:2158169-2159609(+)

Full Sequence      

MLRLATLAVAALTASVVSAGAKCGSGAQCPSSAPCCSQYGECGTGAYCLGGCDPVNSFSL
DSCTPEPICQSKNFNSFDSMDDLASNTKYLGNSTEYDWVYSGTPIVHDGNLVLTMGNGSV
GTLVAYNHYIWYGKVSGTFKTSRDAGVVTAFILLSDAKDEIDYEFIGVDLQTAQTNYYFQ
GIPDWHHGVNATIDSNSFENFHTYEIDWTPDAVTWYLDGKSVRVLKREETYNETTHQYMF
PQTPARLELSLWPGGLASNAQGVIEWAGGEIDWNSQDMQNPGYYYAIFSNISITCYDPPS
WAPSVKDGNAYIYNNIAATNNTVQIVKNNTVLANFADTGLNMTASFSTSTASASQSSSTI
ADSVPGQNGGDGAHSGAGFNGTSSSSSSSTSETSSSAPTSTGFSQGGGSNPKSGTVSVNE
NMLQSSIFAVLVAVVALVAL

Enzyme Prediction     

No EC number prediction in TSTA_016530-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	102	272	2.5e-76	0.9770114942528736

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185692	GH16_fungal_CRH1_transglycosylase	7.58e-90	87	297	1	203	glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
395585	Glyco_hydro_16	1.39e-49	102	268	4	168	Glycosyl hydrolases family 16.
211314	ChtBD1_GH16	5.01e-22	21	67	1	47	Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 16 glycosyl hydrolases. This subfamily includes Saccharomyces cerevisiae Utr2p, also known as Crh2p, which participates in the cross-linking of chitin to beta(1-3)- and beta(1-6) glucan in the cell wall, and S. cerevisiae Crr1p, a putative transglycosidase which is needed for proper spore wall assembly. ChtBD1 is a lectin domain found in proteins from plants and fungi that bind N-acetylglucosamine, plant endochitinases, wound-induced proteins, and the alpha subunit of Kluyveromyces lactis killer toxin. This domain is involved in the recognition and/or binding of chitin subunits; it typically occurs N-terminal to glycosyl hydrolase domains in chitinases, together with other carbohydrate-binding domains, or by itself in tandem-repeat arrangements.
185684	GH16_lichenase	1.20e-19	127	268	63	193	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
185683	Glyco_hydrolase_16	3.81e-18	105	273	34	206	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGA20403.1|CBM18|GH16_19	4.97e-215	1	440	1	425
QKX53550.1|CBM18|GH16_19	9.95e-170	1	440	1	457
BCR85298.1|CBM18|GH16_19	1.84e-141	36	440	36	422
CAP94650.1|CBM18|GH16_19	3.78e-141	2	440	8	433
CAK39569.1|CBM18|GH16_19	3.89e-136	36	439	40	423

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6IBU_A	8.36e-33	129	275	67	209	Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBU_B Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBW_A Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293],6IBW_B Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293]
3D6E_A	2.36e-18	132	268	59	181	Crystal structure of the engineered 1,3-1,4-beta-glucanase protein from Bacillus licheniformis [Bacillus licheniformis],3D6E_B Crystal structure of the engineered 1,3-1,4-beta-glucanase protein from Bacillus licheniformis [Bacillus licheniformis]
1CPM_A	7.88e-18	132	321	14	194	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1GBG_A	1.07e-17	132	268	72	194	Bacillus Licheniformis Beta-Glucanase [Bacillus licheniformis]
1CPN_A	1.30e-17	132	321	14	192	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WI46|CRF2_ASPFU	4.17e-153	2	440	8	443	Probable glycosidase crf2 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=crf2 PE=1 SV=1
sp|P32623|CRH2_YEAST	8.68e-99	17	313	21	317	Probable glycosidase CRH2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UTR2 PE=1 SV=3
sp|Q5AJC0|UTR2_CANAL	1.34e-98	23	339	29	352	Extracellular glycosidase UTR2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=UTR2 PE=1 SV=1
sp|Q05790|CRR1_YEAST	1.43e-56	36	296	44	354	Probable glycosidase CRR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CRR1 PE=2 SV=1
sp|Q75A41|CRR1_ASHGO	6.10e-53	36	297	44	363	Probable glycosidase CRR1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=CRR1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000241	0.999745	CS pos: 19-20. Pr: 0.9795

TMHMM  Annotations     

There is no transmembrane helices in TSTA_016530-t26_1-p1.