CAZyme Information: TRIREDRAFT_121351-t26_1-p1

Basic Information

• Species: Trichoderma reesei
• Lineage: Ascomycota; Sordariomycetes; ; Hypocreaceae; Trichoderma; Trichoderma reesei
• CAZyme ID: TRIREDRAFT_121351-t26_1-p1
• CAZy Family: AA9
• CAZyme Description: glycoside hydrolase family 31

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
964	GL985062|CGC5	109849.34	5.8298

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TreeseiQM6a	9138	431241	7	9131

• Gene Location: location=GL985062:429742-432696(-)

Full Sequence      

MRSTMGLSWKWTALFSLLGAILCLIGPALAVKEHEFKKCHQAGFCNRNRALADLAASQSS
TWVSPYKAVFESPSLEDGKIQGVILKTINAAGDTVRLPVTISFLESGVARLTIDEERRQN
KDIELRHGSAARKERYNEAANWSVVGGLEPALKAEIVHQDDSQINVKYGPEGNFEAVIRL
SPFRIDFRRDGVSHIELNERGLLNVEHWRPKVERPEGEENTEEDESTWWDETFNGHTDTK
PRGPESVALDISFHGYEHVYGIPEHTGPLSLKETRGGEGNYAEPYRMYNADVFEYILDSP
MTLYGSIPFMQAHRKDSSVGLLWLNAADTWVDITKVKGSTNPLSLTSGAPKNTQTHWISE
SGIIDLFVFLGPTPQDITKKYGELTGTTAMPQEFALGYHQCRWNYFSEDDVKDVDRRFDK
AHIPYDVIWLDIEYTDEIKYFTWDPHSFTDPITMGKQLDSHGRKLVTIIDPHIKRVDNYP
INEQLLALDLAIHDKDGKAYEGSCWPGNSNWIDCFNPKAREWWKGLYKYDQFKGTMENTF
IWNDMNEPSVFEGPETTMPKDNLHWDNWEHRDVHNLNGMTYHHSTFEALKSRKKGEYRRP
FVLTRAFFSGSQRFGAMWTGDNLADWGHLQTSVTMLINQGISGFPFSGADVAGFFGDPEK
DLLARWYQTAAFYPFFRAHAHIDTRRREPYLLGEPYTAIVTAALRLRYSLLPAWYTAFFH
ANRDGSPILRPMFWTHPSAEGGLAIDDQFFLASTGLLVKPVAEKDKYSADIWIPDDEVYY
EYDTYNVAKTEQGKHVTFDAPIDRIPILMRGGHIIPRRDIPRRSSSLMRFDPYTLVVSVS
KDGQAEGELYVDDGDSYEYQDGQYIHRQFSLKDDVLSSVDAEGRDTRKIKPGKWLKAMQN
VHIDKIIIVGAPASWDREAVQIESDGRAWAAQVVYHAADKDRAAFATVTHVKARVGDDWS
IKLA

Enzyme Prediction     

EC	3.2.1.20:27	3.2.1.84:26

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	367	815	8.9e-147	0.9953161592505855

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395838	Glyco_hydro_31	0.0	367	815	1	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
269889	GH31_GANC_GANAB_alpha	0.0	386	856	1	467	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.
224418	YicI	2.40e-177	242	879	138	732	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
269890	GH31_glucosidase_II_MalA	2.04e-142	386	725	1	339	Alpha-glucosidase II-like. Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This subgroup also includes the MalA alpha-glucosidase from Sulfolobus solfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source.
215408	PLN02763	3.25e-118	372	865	164	644	hydrolase, hydrolyzing O-glycosyl compounds

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAU87580.1|GH31	0.0	1	964	1	964
UKZ46214.1|GH31	0.0	1	964	1	964
QYS94734.1|GH31	0.0	1	963	1	961
UKZ93782.1|GH31	0.0	1	964	1	965
UKZ70441.1|GH31	0.0	1	964	1	964

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5DKX_A	0.0	31	961	5	948	Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495],5DKY_A Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495],5JQP_A Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495]
5DKZ_A	0.0	31	961	5	948	Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495],5DL0_A Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495]
7KRY_A	1.28e-239	13	911	14	896	Chain A, Neutral alpha-glucosidase AB [Mus musculus],7KRY_C Chain C, Neutral alpha-glucosidase AB [Mus musculus]
5IEF_A	3.77e-239	31	911	1	865	Murine endoplasmic reticulum alpha-glucosidase II with N-butyl-1-deoxynojirimycin [Mus musculus]
5IEG_A	3.89e-239	31	911	1	865	Murine endoplasmic reticulum alpha-glucosidase II with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9US55|GLU2A_SCHPO	9.23e-284	21	910	16	885	Glucosidase 2 subunit alpha OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gls2 PE=1 SV=1
sp|Q94502|GANAB_DICDI	8.81e-245	20	910	11	897	Neutral alpha-glucosidase AB OS=Dictyostelium discoideum OX=44689 GN=modA PE=3 SV=1
sp|Q9FN05|PSL5_ARATH	1.10e-242	20	910	9	865	Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1
sp|Q14697|GANAB_HUMAN	1.30e-241	22	911	25	897	Neutral alpha-glucosidase AB OS=Homo sapiens OX=9606 GN=GANAB PE=1 SV=3
sp|Q4R4N7|GANAB_MACFA	1.30e-241	22	911	25	897	Neutral alpha-glucosidase AB OS=Macaca fascicularis OX=9541 GN=GANAB PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000318	0.999655	CS pos: 30-31. Pr: 0.9740

TMHMM  Annotations     

Start	End
7	29