CAZyme Information: TREMEDRAFT_69207-t26_1-p1

Basic Information

• Species: Tremella mesenterica
• Lineage: Basidiomycota; Tremellomycetes; ; Tremellaceae; Tremella; Tremella mesenterica
• CAZyme ID: TREMEDRAFT_69207-t26_1-p1
• CAZy Family: GT39
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
463	JH711534|CGC3	51578.98	4.3578

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TmesentericaDSM1558	8309	578456	1	8308

• Gene Location: location=JH711534:1035471-1038637(-)

Full Sequence      

MGPTANLGMRSVSAGMSTTGGLGRYIIGTPGYNLGGINGSGQYPYTGLPTLIDPETPMDV
YKRTGSDGEEWNLVFSDEFNKDGRTFYNGDDPFFEAVDLHYWPTNDFEWYDPSAITTQDG
HLVITMTQEPIHDLNFKSGMLQSWNKLCFNTNAYFEVSASLPGSPNINGWWPGIWTMGNL
GRPGYGGTTDGMWPYTYDSCDIGTLANQTYLNGTGPEGALFSGKDSTSISYLPGQRTSAC
TCKGEDHPGPKESIGRGIPEIDMLEAQIDIMRGIGQVSQSFQTAPFDDYYQFNNLTPVWN
NYLGGVYQQAISSLTDIPRDSYWGTKKEFTVFGFEYKSNKLKREDGYVTWYVDGNESWTL
YGSAVGPNDRMGIGQRLVSEEPMALSNNFANVDFNHMTWPNYLRIDYLRVYQNKDGTVGC
DPADHPTADYIARHAEVYNNPNITTWTGAGYPFPKNRLKDDCT

Enzyme Prediction     

No EC number prediction in TREMEDRAFT_69207-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	77	411	2.4e-128	0.9969325153374233

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	0.0	28	462	72	500	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	2.00e-143	73	412	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	7.45e-40	51	424	29	355	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	3.92e-12	75	411	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185691	GH16_Strep_laminarinase_like	0.002	71	194	2	126	Streptomyces laminarinase-like, member of glycosyl hydrolase family 16. Proteins similar to Streptomyces sioyaensis beta-1,3-glucanase (laminarinase) present in Actinomycetales as well as Peziomycotina. Laminarinases belong to glycosyl hydrolase family 16 and hydrolyze the glycosidic bond of the 1,3-beta-linked glucan, a major component of fungal and plant cell walls and the structural and storage polysaccharides (laminarin) of marine macro-algae. Members of the GH16 family have a conserved jelly roll fold with an active site channel.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
KGB80226.3|GH16_2	1.32e-228	29	462	169	623
AAW43075.1|GH16_2	3.06e-227	29	462	169	623
ADV24708.1|GH16_2	6.15e-227	29	462	169	623
AUB25004.1|GH16_2	2.40e-226	29	462	168	622
AFR95233.2|GH16_2	2.40e-226	29	462	168	622

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JH5_A	4.23e-07	69	415	2	235	Structure of Marine bacterial laminarinase [Aquimarina]
6JHJ_A	1.02e-06	69	415	2	235	Structure of Marine bacterial laminarinase mutant-E135A [Aquimarina],6JIA_A Marine bacterial laminarinase mutant E135A complex with laminaritetraose [Aquimarina sp.],6M6P_A Structure of Marine bacterial laminarinase mutant E135A in complex with 1,3-beta-cellotriosyl-glucose [Aquimarina sp.]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O13941|YEPB_SCHPO	1.76e-131	51	462	218	627	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1
sp|P87024|SKN1_CANAX	4.53e-128	50	458	334	734	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P32486|KRE6_YEAST	3.54e-126	42	463	293	712	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P33336|SKN1_YEAST	4.86e-126	42	463	347	764	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P87023|KRE6_CANAX	3.23e-122	51	458	331	737	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000069	0.000007

TMHMM  Annotations     

There is no transmembrane helices in TREMEDRAFT_69207-t26_1-p1.