CAZyme Information: TREMEDRAFT_32575-t26_1-p1

Basic Information

• Species: Tremella mesenterica
• Lineage: Basidiomycota; Tremellomycetes; ; Tremellaceae; Tremella; Tremella mesenterica
• CAZyme ID: TREMEDRAFT_32575-t26_1-p1
• CAZy Family: GH1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1176	JH711535|CGC6	131746.90	6.3656

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TmesentericaDSM1558	8309	578456	1	8308

• Gene Location: location=JH711535:797790-802494(+)

Full Sequence      

MSFWRRYKSLSQRPRTLGLISVIFVLTVFLFHSIPQDDLYRDDHPQQRVIIPVGPQTWEE
WNDGEEDTPSNKGGMGSWLKVKTPKLGLEGLGLKKGGKRSLLVTGGAGQLGLALVNSLVA
DYTIHIVDIAKRPSFLTKSPNIIYHRGSILPPSDTLSSLFATTSFDGVVHLAAISLEAWC
EPKEIECLTVNEGGTKAVLDQLDILRQKQPRRALLSWGKRKVPWMILASSMDVYGSSEFM
GGSVDETTLRQPINAIGRTKLLAEQAVEEAATRGSEQQTKMPLKAAIVRFSDVYGYPHGT
TITQSFFPSLVIDAITSLPIQYSSSTPPMDLIHVDDAVAGLTKVIRRLSTQSDPNMGGLE
NFNIVSGGKRWNSEEIVDLVRAQTGSMSPLRDIGDHLAAFAPVAEYSNIKASTDLDWQPT
TSLTVGLGRTVTALSSEMAHYTRAFHSDNCPPSADYPSLDGLQHPQPEDERNRDLSKLDG
CTVGMGFNHDGWIHHVKCEDGRFCGADGNKVIAYNWNASVWIVKAQRSDVVRERFLRVRF
EEETGAGTLGISEENWDAGIVKLELFPPDREDDAAILTFDIEVSESSSSLRVMIPNSEQQ
LHAVANTTDSSTSFVMEHLTKFARPHYDMRLTVICCPSVGDWPLLLDDFETADILYGSTG
QIPFNASRRSSQCTRAAKAVEYNSNKLSSEEKVLDAMASPPQLGESLQDSLQVSTQLSKL
PNDWGLAHLPPCWNECHSPTVCIQSGDCRCVKADHCRPRRENPLLYQFKTMKKNPIEIMF
DSHRLSNEVKQKDWKDILLPKAKSYFTQHDKFPKVHIVNGYPEEKKIELAECHKLQPAHC
FSADNILYRAMRHISVSAEEADLIVLPVYQHCTDEEFMLHDLTAFAVKTIPGVLNQDKKL
AFVLTHDWGICVNFAWEIWSARGESHLTPDWILRNALVWSVMGDYNSPCYRPHQDIVVPP
RTCKSIDLREHFPEITNVTPMRQRTKLVTWSGTYWGTGKNMRLRLTCERGGAGKEELVPG
GGPMSSWYNWEYMKEISGARFCPQPTGIAGWSPRINDAIYAGCIPVLTAEGTHYPFADFL
DWSKFSIRIKPTELDQLERILSAIPLEQLEEMQANLMLVREAFIYSTDENPEDELKRRGP
MFFALHEAGMRLRTLYPVGKNGVSKDTIYRQPVKGG

Enzyme Prediction     

No EC number prediction in TREMEDRAFT_32575-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT47	813	1104	9.6e-37	0.9831081081081081

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397245	Exostosin	1.23e-51	809	1104	1	290	Exostosin family. The EXT family is a family of tumor suppressor genes. Mutations of EXT1 on 8q24.1, EXT2 on 11p11-13, and EXT3 on 19p have been associated with the autosomal dominant disorder known as hereditary multiple exostoses (HME). This is the most common known skeletal dysplasia. The chromosomal locations of other EXT genes suggest association with other forms of neoplasia. EXT1 and EXT2 have both been shown to encode a heparan sulphate polymerase with both D-glucuronyl (GlcA) and N-acetyl-D-glucosaminoglycan (GlcNAC) transferase activities. The nature of the defect in heparan sulphate biosynthesis in HME is unclear.
223528	WcaG	2.20e-32	102	431	4	305	Nucleoside-diphosphate-sugar epimerase [Cell wall/membrane/envelope biogenesis].
212494	SDR_e	1.70e-24	102	364	2	199	extended (e) SDRs. Extended SDRs are distinct from classical SDRs. In addition to the Rossmann fold (alpha/beta folding pattern with a central beta-sheet) core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids. Extended SDRs are a diverse collection of proteins, and include isomerases, epimerases, oxidoreductases, and lyases; they typically have a TGXXGXXG cofactor binding motif. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.
396097	Epimerase	3.15e-23	102	347	2	227	NAD dependent epimerase/dehydratase family. This family of proteins utilize NAD as a cofactor. The proteins in this family use nucleotide-sugar substrates for a variety of chemical reactions.
187558	UDP_G4E_1_SDR_e	4.41e-16	101	423	2	311	UDP-glucose 4 epimerase, subgroup 1, extended (e) SDRs. UDP-glucose 4 epimerase (aka UDP-galactose-4-epimerase), is a homodimeric extended SDR. It catalyzes the NAD-dependent conversion of UDP-galactose to UDP-glucose, the final step in Leloir galactose synthesis. This subgroup has the characteristic active site tetrad and NAD-binding motif of the extended SDRs. Extended SDRs are distinct from classical SDRs. In addition to the Rossmann fold (alpha/beta folding pattern with a central beta-sheet) core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids. Extended SDRs are a diverse collection of proteins, and include isomerases, epimerases, oxidoreductases, and lyases; they typically have a TGXXGXXG cofactor binding motif. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAW44475.1|GT47	0.0	12	1158	16	1128
AFR96705.1|GT47	0.0	12	1158	16	1121
AUB26654.1|GT47	0.0	12	1158	16	1121
KGB76296.2|GT47	0.0	45	1158	42	1126
UOH83106.1|GT47	0.0	12	1158	16	1121

PDB Hits     

TREMEDRAFT_32575-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q8S1X9|GT13_ORYSJ	2.16e-19	839	1133	81	381	Probable glucuronosyltransferase Os01g0926400 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0926400 PE=2 SV=1
sp|Q33AH8|GT101_ORYSJ	6.66e-18	838	1133	75	376	Probable glucuronosyltransferase GUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=GUT1 PE=2 SV=2
sp|Q6H4N0|GT21_ORYSJ	5.79e-17	839	1133	92	392	Probable glucuronosyltransferase Os02g0520750 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0520750 PE=2 SV=1
sp|Q7XLG3|GT42_ORYSJ	2.90e-16	839	1133	79	379	Probable glucuronosyltransferase Os04g0398600 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0398600 PE=2 SV=2
sp|Q10SX7|GT31_ORYSJ	7.25e-16	839	1126	99	394	Probable glucuronosyltransferase Os03g0107900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0107900 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000049	0.000002

TMHMM  Annotations     

Start	End
17	34