CAZyme Information: THC99188.1

Basic Information

• Species: Aspergillus tanneri
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus tanneri
• CAZyme ID: THC99188.1
• CAZy Family: GT25
• CAZyme Description: Alpha,alpha-trehalase [Source:UniProtKB/TrEMBL;Acc:A0A4S3JUV3]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1071		117097.77	4.6781

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AtanneriNIH1004	13590	N/A	33	13557

• Gene Location: location=SOSA01000024:83803-87194(+)

Full Sequence      

MKLKVTAALGLLSSLPGAVCSSSSGKNDRITNALNRHQSGHGPRKAAASSTSLYETRFDG
LTWDDNNWILQTTTLDQGRYQSRGSVANGYLGISVASVGPFFEVDIEDNGNEIDGWPLFA
RRQTFATIAGFFDSQSTTNSTNFGWLYQYGWDSVISGVPHWGGLILDLGDDTYLDASVDN
STIKNFHSMYDFKAGVLSWSYDWVPKGDKGSFAITYRLFANKLNVTQAVVDMEITPSQDS
DATVVNIIDGKSAARTDFAESGQDDSAIFTAVRPWGISNVTAYVYANLTASNGVDLSSRA
LITGKPYMSDNKSTIAEAVPVKFAANEAVRVTKFVGAASSDAFQNPRQIAKNAASAALVA
GYQKSLTSHVSEWASVMPDDSVEHFVDPATGKLPDDDHIIDSAIIAVANTYYLLQTTVGP
RAIAAVSGAPVNRDSIAVGGLTSDAYAGQIFWDADVWMQPGLVASHPESAQRVTNYRVAK
FEQAQANIETAFAGSKNRTKFEPSAAIYPWTSGRFGNCTATGPCWDYQYHLNGDIGLSMI
YEWVASGDTPHFRDNHFPVYDSVATLYSNLVERNGSTWTLTNMTDPDEYANHVDAGAFTM
AMISETLENANIFREKFDQETNETWSEISDNVLMIQENDVTLEYTTMNGTAVVKQADIVL
MTYPLAYDSYSHETALNDLDYYAIKQSADGPAMTWAIFSIVASDIAQSGCSAYTYHQYSY
SPYARAPFYQLSEQMIDDPNVNGGTHPAFPFLTGHGGANQVALFGYLGLRLVPDDILHID
PNLPPQIPYVRYRTFYWHGWPISAHSNYTHTTIQRAKDVPAMKTADRRFANSTISVHVGE
AQNSTVYTLPLTSSLTIPNRQTGTIPTIDGNLVQCRPVYSPDDFEPGQFPISAVDGANST
KWQPSTSNLTSLTVTLSDAEIKSNSLVSGFHFDWSGSPPVNATVIFHDEFIENPATAVLE
NSNSPRHNLVSQLTNIKLSEPYSALKNYNEVTTPVGNETTFQLENPVPVSRYATLLISGN
QAQDDEEKGATVAEWVILGQDQGSSDKKDGVRKLSVKNAVALERGLGRRRY

Enzyme Prediction     

EC	3.2.1.28:5	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH65	409	771	2.4e-80	0.9838709677419355

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224471	ATH1	3.20e-63	67	796	10	724	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
397616	Glyco_hydro_65N	6.03e-37	76	340	9	240	Glycosyl hydrolase family 65, N-terminal domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. This domain is believed to be essential for catalytic activity although its precise function remains unknown.
281612	Glyco_hydro_65m	8.12e-23	409	771	3	382	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMW44967.1|GH65	0.0	11	1064	8	1067
UDD58012.1|GH65	0.0	11	1064	12	1071
QRD84477.1|GH65	0.0	11	1064	8	1067
QMW32935.1|GH65	0.0	11	1064	8	1067
BAE62687.1|GH65	0.0	11	1064	12	1067

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7FE3_A	7.68e-33	154	771	74	642	Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_A Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101]
3WIQ_A	1.06e-15	435	799	326	722	Crystal structure of kojibiose phosphorylase complexed with kojibiose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_A Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_B Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_C Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_D Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903]
4KTP_A	4.08e-10	425	784	305	703	Chain A, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTP_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]
4KTR_A	9.25e-10	425	784	305	703	Chain A, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_C Chain C, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_D Chain D, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_E Chain E, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_F Chain F, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_G Chain G, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_H Chain H, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P78617|TREA_EMENI	0.0	9	1048	10	1047	Acid trehalase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=treA PE=3 SV=1
sp|D4B5H9|ATC1_ARTBC	0.0	54	1038	53	1051	Cell wall acid trehalase ARB_03719 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_03719 PE=1 SV=1
sp|Q5AAU5|ATC1_CANAL	5.24e-112	59	944	99	964	Cell wall acid trehalase ATC1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ATC1 PE=1 SV=2
sp|M1RNJ8|ATH1_CANGB	1.34e-110	6	1037	100	1210	Periplasmic/secreted acid trehalase ATH1 OS=Candida glabrata OX=5478 GN=ATH1 PE=1 SV=1
sp|P48016|ATH1_YEAST	4.44e-104	30	964	92	1059	Periplasmic acid trehalase ATH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ATH1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.011876	0.988089	CS pos: 20-21. Pr: 0.6747

TMHMM  Annotations     

There is no transmembrane helices in THC99188.1.