CAZyme Information: THC91878.1

Basic Information

• Species: Aspergillus tanneri
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus tanneri
• CAZyme ID: THC91878.1
• CAZy Family: GH13
• CAZyme Description: Autophagy-related protein 26 [Source:UniProtKB/TrEMBL;Acc:A0A4S3JC35]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1161		130020.22	6.6290

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AtanneriNIH1004	13590	N/A	33	13557

• Gene Location: location=SOSA01000376:629-4328(+)

Full Sequence      

MVEAQALLVPEAAAERSQSSIERSDKDPQQPGSSALSLRLMEMFGYECPEKVIVEYACSL
LQSMLLQGYMYVTEGHICFYAYLPRKSTVAIKSGWLHKRGRKNPKYGRYWFSLKGDVLSY
YADPSNFYFPSGHVDLRYGISASLAEQKDKSKETKDFQVTTDQRTYYFRADSHASAKEWV
KALQKVIFRTHNDGESVKVSFPIGNIIDIEESPMVDFAETFKLRVVDRDTYAIDEYYFSV
FNTGQDAYNFLKGLINETTTKNSQTLSSKQDHTANHGRSRGYQNRWSLSNVLSQPRGIGN
VGSQQRSVSTSHISFGHDVVGTSPPTKNPEPSDSFVNSFEQATESSAVLQSMTDTTESAS
QILNRSDVFQSPTIHTLRHRPSDTDRTGRRHSDETARSISNAVADSSTFPRRHGHGIQYA
ISESDQETQGASKVHTSAPSLNELVRAGVYPLQRAAGLAEYLKNRSKQMSTLLASESMGY
LEKVSGMWAGGRKHYGEGEGVQPDDQDVDPEDREDGCGHGDRFRAHFTLPSTERLQATYF
AYLHRVLPLYGKIYISQKKLCFRSLIPGTRTKMILPLKDIENVEKEKGFRFGYHGLVVVI
RGHEELFFEFNTSDARDDCAVTLHQGLESVRFLAESGMLAEQEKDESEAAKAEHLMLQEA
RKDATGQYDTRTILDESSELHPIFDDTRASIINFKPSEPLRITCLTIGSRGDVQPYIALC
KGLLAEGHKPKIATHAEFEPWIRKHGIDFAPVDGDPAELMRICVENESPSAMAGIHIAEA
LRIPYFRAFTMPWSRTRAYPHAFAVPEHKLGGSYNHITYIMFDTVFWKAIAGQVNRWRKN
ELGLKATNLDKMQPNKVPFLYNYSPSVVAPPLDYPDWIRVTGYWFLSEGTDWSPPAELTD
FIQRARKDGKKIVYIGFGSIVVSDPSALTRTVIESVQKADVRCILSKGWSDRLGDPASAK
PEIPLPPEIMQIQAAPHDWLFAQIDAAAHHGGAGTTGASLRAGVPTIVKPFFGDQFFFGN
RVQDLGVGICMKKLNVSVFSRALWEATHSERMIMRARELGVKIRSENGVETAIQAVYRDL
EYAKTLARQRSIVSSTPFSPTPSAKTIAEQEEDDGMTDSEEWTFVGDDTDIDISRRVRDR
AISDADMLPEPVSGYASTKIA

Enzyme Prediction     

EC	2.4.1.173:13

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT1	744	1076	2.8e-48	0.7670157068062827

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340817	GT1_Gtf-like	1.60e-66	700	1076	1	401	UDP-glycosyltransferases and similar proteins. This family includes the Gtfs, a group of homologous glycosyltransferases involved in the final stages of the biosynthesis of antibiotics vancomycin and related chloroeremomycin. Gtfs transfer sugar moieties from an activated NDP-sugar donor to the oxidatively cross-linked heptapeptide core of vancomycin group antibiotics. The core structure is important for the bioactivity of the antibiotics.
275402	PH-GRAM1_AGT26	1.86e-56	70	187	1	116	Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, repeat 1. ATG26 (also called UGT51/UDP-glycosyltransferase 51), a member of the glycosyltransferase 28 family, resulting in the biosynthesis of sterol glucoside. ATG26 in decane metabolism and autophagy. There are 32 known autophagy-related (ATG) proteins, 17 are components of the core autophagic machinery essential for all autophagy-related pathways and 15 are the additional components required only for certain pathways or species. The core autophagic machinery includes 1) the ATG9 cycling system (ATG1, ATG2, ATG9, ATG13, ATG18, and ATG27), 2) the phosphatidylinositol 3-kinase complex (ATG6/VPS30, ATG14, VPS15, and ATG34), and 3) the ubiquitin-like protein system (ATG3, ATG4, ATG5, ATG7, ATG8, ATG10, ATG12, and ATG16). Less is known about how the core machinery is adapted or modulated with additional components to accommodate the nonselective sequestration of bulk cytosol (autophagosome formation) or selective sequestration of specific cargos (Cvt vesicle, pexophagosome, or bacteria-containing autophagosome formation). The pexophagosome-specific additions include the ATG30-ATG11-ATG17 receptor-adaptors complex, the coiled-coil protein ATG25, and the sterol glucosyltransferase ATG26. ATG26 is necessary for the degradation of medium peroxisomes. It contains 2 GRAM domains and a single PH domain. PH domains are only found in eukaryotes. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. PH domains also have diverse functions. They are often involved in targeting proteins to the plasma membrane, but few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.
275403	PH-GRAM2_AGT26	5.04e-55	532	624	1	93	Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, repeat 2. ATG26 (also called UGT51/UDP-glycosyltransferase 51), a member of the glycosyltransferase 28 family, resulting in the biosynthesis of sterol glucoside. ATG26 in decane metabolism and autophagy. There are 32 known autophagy-related (ATG) proteins, 17 are components of the core autophagic machinery essential for all autophagy-related pathways and 15 are the additional components required only for certain pathways or species. The core autophagic machinery includes 1) the ATG9 cycling system (ATG1, ATG2, ATG9, ATG13, ATG18, and ATG27), 2) the phosphatidylinositol 3-kinase complex (ATG6/VPS30, ATG14, VPS15, and ATG34), and 3) the ubiquitin-like protein system (ATG3, ATG4, ATG5, ATG7, ATG8, ATG10, ATG12, and ATG16). Less is known about how the core machinery is adapted or modulated with additional components to accommodate the nonselective sequestration of bulk cytosol (autophagosome formation) or selective sequestration of specific cargos (Cvt vesicle, pexophagosome, or bacteria-containing autophagosome formation). The pexophagosome-specific additions include the ATG30-ATG11-ATG17 receptor-adaptors complex, the coiled-coil protein ATG25, and the sterol glucosyltransferase ATG26. ATG26 is necessary for the degradation of medium peroxisomes. It contains 2 GRAM domains and a single PH domain. PH domains are only found in eukaryotes. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. PH domains also have diverse functions. They are often involved in targeting proteins to the plasma membrane, but few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.
224732	YjiC	6.55e-44	701	1072	3	391	UDP:flavonoid glycosyltransferase YjiC, YdhE family [Carbohydrate transport and metabolism].
397160	GRAM	2.61e-26	522	619	2	99	GRAM domain. The GRAM domain is found in in glucosyltransferases, myotubularins and other putative membrane-associated proteins. Note the alignment is lacking the last two beta strands and alpha helix.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMW35213.1|GT1	0.0	1	1153	193	1378
QMW47275.1|GT1	0.0	1	1153	193	1378
QRD91604.1|GT1	0.0	1	1153	196	1381
BAE64992.1|GT1	0.0	1	1153	256	1441
UDD64301.1|GT1	0.0	1	1153	196	1381

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XVM_A	6.56e-160	693	1125	13	478	Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) [Saccharomyces cerevisiae S288C],5XVM_B Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) [Saccharomyces cerevisiae S288C]
5GL5_A	1.30e-159	693	1125	33	498	Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c): UDPG complex [Saccharomyces cerevisiae S288C],5GL5_B Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c): UDPG complex [Saccharomyces cerevisiae S288C]
3H4I_A	3.62e-17	708	1029	9	330	Chimeric Glycosyltransferase for the generation of novel natural products [Amycolatopsis orientalis],3H4T_A Chimeric Glycosyltransferase for the generation of novel natural products - GtfAH1 in complex with UDP-2F-Glc [Amycolatopsis orientalis]
1RRV_A	1.21e-13	700	1029	1	348	X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin. [Amycolatopsis orientalis],1RRV_B X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin. [Amycolatopsis orientalis]
6KQW_A	5.11e-10	702	1072	6	377	Chain A, Uncharacterized UDP-glucosyltransferase YjiC [Bacillus subtilis subsp. subtilis str. 168]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0CKU4|ATG26_ASPTN	0.0	1	1153	197	1388	Sterol 3-beta-glucosyltransferase OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=atg26 PE=3 SV=1
sp|I1S8Q3|ATG26_GIBZE	0.0	1	1158	202	1441	Sterol 3-beta-glucosyltransferase ATG26 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=ATG26 PE=3 SV=1
sp|Q5B4C9|ATG26_EMENI	0.0	1	1150	201	1383	Sterol 3-beta-glucosyltransferase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=atg26 PE=3 SV=1
sp|Q4WID6|ATG26_ASPFU	0.0	1	1150	195	1389	Sterol 3-beta-glucosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=atg26 PE=3 SV=2
sp|C4B4E5|ATG26_GLOLA	0.0	34	1128	263	1434	Sterol 3-beta-glucosyltransferase OS=Glomerella lagenarium OX=5462 GN=ATG26 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000062	0.000000

TMHMM  Annotations     

There is no transmembrane helices in THC91878.1.