CAZyme Information: SSD60786.1

Basic Information

• Species: Saccharomycodes ludwigii
• Lineage: Ascomycota; Saccharomycetes; ; Saccharomycodaceae; Saccharomycodes; Saccharomycodes ludwigii
• CAZyme ID: SSD60786.1
• CAZy Family: GT15
• CAZyme Description: Chitinase [Source:UniProtKB/TrEMBL;Acc:A0A376B7X3]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1240		125748.60	3.8617

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SludwigiiUTAD17	4017	N/A	2	4015

• Gene Location: location=UFAJ01000453:1779-5501(+)

Full Sequence      

MKFVNFALFISAIATILISTVSCAFDVNSKKNVVAYWGQSAVYYENGEAQSSLSSYCSSG
NVDIIVMSFVIEFFPTTINLGNYCDSTTCTNVASDIKSCQDQGVKVMLSLGGASGSYGFS
SDSEAEEYATTLWNLFGAGTSASNLERPFSDAVVDGFDLDIENNNYVGYTAFVDKLRDYF
ATDSSKDYYISAAPQCPFPDYNVGPALANSLIDFAFIQFYNNAGCNADGSDFNFATWSSK
VESGVIKNPDIKLFVGLPGASTSASTGYISDYTTLQSIVNEAAAYAEFGGISLWDVSSAT
ANTNVDGQNYILDVKQALADAVVASSSIAASSSLSSSITPSSSVPPSSSVAPSSATVLSS
SVVLSSSVPLTSAIPSSSTVPSSSVVLYSSVVLYSSVPSSSAIPSSSAIPSSSAIPSSSA
IPSSSVLSSSVVLSSSVPLSSAIPSSSVLPSSSIVPSSSVPLSSAIPSGSGIPSSSVLPS
SSIVPSSSVPPSSSIIPSSSVVLSSSVAPLSATVLSSSVVLSSSVPLTSAIPSSSIVPSS
SVPLSSAIPSSSVLPSSSIVPSSSVPSSSIVPSSSVLSSSVVLSSSVPLTSAIPSSSVLP
SSSIVPSSSVPLSSAIPSGSGIPSSSVLSSSSVPLSSAIPSSFVPPSSSDLSSFVPLSSS
IILSNSVVLSSSVAPSSATVPSSSTVVSSSVPLTSAIPSSSTVASNSVPLSSGIPSSSGI
PSSSVPSSFAIPSSSIVPSSSVSPSSSVVLSSSVAPSSATVLSSSVVLSSSVPLTPAIPS
SSPVLSSASITFGPSIPSSSVVLSNSIPSSSIPLNSVTESNLAMSSSFAVYSKSVIVSFS
TITRYSTSATPSNSVADLSLNNIEYSDVVSSSSSALSNYHTSNLEVSCSSTIVLVPTTSL
TTYTSLVLPTTSSGVSSSISLDNYFAVSSNSLSGIISSATSQFSISSFQIAIPNSKSSFY
SVTALTPVTTRKTAVLTPTVSLITDLDRETQEFAPTITTTYPKNEFVTESSSDILFSTAA
TISESSYVLATSFTSCVSSDFSTSVTRDEFDSFTNSLKVTSSVENSIGSFSTLAASNEAE
NMETLVTIVATATAFTTIVTPSVTTENVTVCKNNYCSEYLVTFTSTESKVLYSTEAVIGY
KTIITSSANAVQTPQLQSSVSYSNSTLLTTVLTSISQQTPIIQAQNSNSTEPVSTGNYIS
STPTPSVDVQSINGSISIINNVKTTSIFTIILSIITVILI

Enzyme Prediction     

EC	3.2.1.14:12

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	30	297	1.1e-27	0.7804054054054054

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119356	GH18_hevamine_XipI_class_III	1.31e-95	32	304	2	273	This conserved domain family includes xylanase inhibitor Xip-I, and the class III plant chitinases such as hevamine, concanavalin B, and PPL2, all of which have a glycosyl hydrolase family 18 (GH18) domain. Hevamine is a class III endochitinase that hydrolyzes the linear polysaccharide chains of chitin and peptidoglycan and is important for defense against pathogenic bacteria and fungi. PPL2 (Parkia platycephala lectin 2) is a class III chitinase from Parkia platycephala seeds that hydrolyzes beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin.
395573	Glyco_hydro_18	5.91e-25	32	297	1	305	Glycosyl hydrolases family 18.
119349	GH18_chitinase-like	8.54e-15	33	221	1	177	The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model.
226000	Chi1	1.02e-11	95	319	90	326	Chitinase [Carbohydrate transport and metabolism].
119363	GH18_CTS3_chitinase	8.31e-09	100	295	69	252	GH18 domain of CTS3 (chitinase 3), an uncharacterized protein from the human fungal pathogen Coccidioides posadasii. CTS3 has a chitinase-like glycosyl hydrolase family 18 (GH18) domain; and has homologs in bacteria as well as fungi.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCH61165.1|CBM19|GH18	2.03e-90	15	320	13	312
CCC69882.1|CBM19|GH18	3.37e-87	24	318	20	308
CCF55925.1|CBM19|GH18	3.42e-87	6	327	4	318
CAH01261.1|CBM19|GH18|3.2.1.14	9.25e-86	24	327	19	318
QEU60125.1|CBM19|GH18	1.75e-85	24	327	19	318

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2UY2_A	2.63e-84	26	321	1	290	ScCTS1_apo crystal structure [Saccharomyces cerevisiae],2UY3_A ScCTS1_8-chlorotheophylline crystal structure [Saccharomyces cerevisiae],2UY4_A ScCTS1_acetazolamide crystal structure [Saccharomyces cerevisiae],2UY5_A ScCTS1_kinetin crystal structure [Saccharomyces cerevisiae],4TXE_A ScCTS1 in complex with compound 5 [Saccharomyces cerevisiae]
4TX6_A	1.10e-49	30	318	1	308	AfChiA1 in complex with compound 1 [Aspergillus fumigatus A1163],4TX6_B AfChiA1 in complex with compound 1 [Aspergillus fumigatus A1163]
2XVN_A	1.97e-49	32	318	2	307	A. fumigatus chitinase A1 phenyl-methylguanylurea complex [Aspergillus fumigatus],2XVN_B A. fumigatus chitinase A1 phenyl-methylguanylurea complex [Aspergillus fumigatus],2XVN_C A. fumigatus chitinase A1 phenyl-methylguanylurea complex [Aspergillus fumigatus]
2XTK_A	2.03e-49	32	318	3	308	ChiA1 from Aspergillus fumigatus in complex with acetazolamide [Aspergillus fumigatus A1163],2XTK_B ChiA1 from Aspergillus fumigatus in complex with acetazolamide [Aspergillus fumigatus A1163],2XUC_A Natural product-guided discovery of a fungal chitinase inhibitor [Aspergillus fumigatus],2XUC_B Natural product-guided discovery of a fungal chitinase inhibitor [Aspergillus fumigatus],2XUC_C Natural product-guided discovery of a fungal chitinase inhibitor [Aspergillus fumigatus],2XVP_A ChiA1 from Aspergillus fumigatus, apostructure [Aspergillus fumigatus A1163],2XVP_B ChiA1 from Aspergillus fumigatus, apostructure [Aspergillus fumigatus A1163]
1HVQ_A	5.34e-46	33	294	3	255	CRYSTAL STRUCTURES OF HEVAMINE, A PLANT DEFENCE PROTEIN WITH CHITINASE AND LYSOZYME ACTIVITY, AND ITS COMPLEX WITH AN INHIBITOR [Hevea brasiliensis],1LLO_A Chain A, HEVAMINE [Hevea brasiliensis],2HVM_A Hevamine A At 1.8 Angstrom Resolution [Hevea brasiliensis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P29029|CHIT_YEAST	1.99e-81	24	321	20	311	Endochitinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CTS1 PE=1 SV=2
sp|P40954|CHI3_CANAL	6.43e-76	24	321	16	313	Chitinase 3 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CHT3 PE=1 SV=2
sp|Q5AAH2|CHI1_CANAL	5.01e-73	27	319	14	289	Chitinase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CHT1 PE=1 SV=1
sp|P46876|CHI1_CANAX	6.85e-73	27	319	14	289	Chitinase 1 OS=Candida albicans OX=5476 GN=CHT1 PE=3 SV=2
sp|P40953|CHI2_CANAL	8.00e-68	28	327	20	312	Chitinase 2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CHT2 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.037791	0.962181	CS pos: 23-24. Pr: 0.7530

TMHMM  Annotations     

There is no transmembrane helices in SSD60786.1.