CAZyme Information: SSD59553.1

Basic Information

• Species: Saccharomycodes ludwigii
• Lineage: Ascomycota; Saccharomycetes; ; Saccharomycodaceae; Saccharomycodes; Saccharomycodes ludwigii
• CAZyme ID: SSD59553.1
• CAZy Family: GH18
• CAZyme Description: related to Beta-glucan synthesis-associated protein KRE6

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
798	UFAJ01000160|CGC1	90406.61	4.4027

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SludwigiiUTAD17	4017	N/A	2	4015

• Gene Location: location=UFAJ01000160:12533-14929(+)

Full Sequence      

MTNNREKSKRILTSSSSNNEHLSQQQNIASTSNNNDASSLTQLESQNPFSDENAYDSSGS
NLPPSSSDTLNNNNHNNNNLSSESLLHNQNLAFLNDSSNKNRNPNEMENSRRKHDNNKAR
SDNYYDKNYNNRNMIEEGEEEEEEEEEEFAFNDRDADTHENNTYTGYYSSQLLSKSNKNL
MEKAKTKKNSVMSLENTDLSFLSRYDRYPALENNDNNKHMKPSTVFTSIIHGNNNNNNNN
NNSSTTSHSSFSSQSGTFNKKKNFASNHYLMNEQDFSPFGGYPASSFPLLMEEKEDDDYL
HNPDPEEEARLDRQRFYLDFKHMDRRSFGGLIGSLFLICGTVVVFIILPILTYSGIGTHG
KYTEVINILTLYEYPQLSAIRTSLVDPDTPSDAMAHKAKDGSYWKLAFSDEFNALGRTFY
DGDDQFWTAPDLNYKATNDLEWYSPDAATTFNGTLQLRMDAFETHDLYYRSGMLQSWNKL
CFTQGYIEVSANLPNYGRVSGLWPGLWTMGNLGRPGYLASTEGLWPYSYDSCDAGITPNQ
SSPDGISYLPGQKLSICTCDGEDHPNQGVGRGAPELDILEGEVDTATKGGVGLASQSMQV
APFDIWYMPDYDYIEIYNQTTTTINTYAGGPFQQAVSAVTTLNNSWYEYGPDAGYFQRFG
FEYLNDDEDGYVRWFVGETPTCTIYAKALHPNGNIGWRRITKEPMSIILNLGISNNWAYI
DWQSIYFPVTLSIDYVRIYQPNDSISITCDPEDYPTYDYIQDHLNVYTNVNLTSWEGAGY
TFPKNILTGNCEIGRAHV

Enzyme Prediction     

No EC number prediction in SSD59553.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	410	739	9.3e-160	0.9969325153374233

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	0.0	287	791	1	500	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	8.76e-175	406	740	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	9.42e-95	369	753	14	355	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	1.29e-23	408	739	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185683	Glyco_hydrolase_16	2.24e-07	410	517	1	97	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCC70849.1|GH16_2	0.0	262	795	190	721
CCD23627.1|GH16_2	0.0	252	795	207	749
QLQ80092.1|GH16_2	0.0	252	795	178	718
QLL32994.1|GH16_2	0.0	252	795	179	719
QLG71197.1|GH16_2	0.0	253	795	174	710

PDB Hits     

SSD59553.1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P32486|KRE6_YEAST	1.41e-318	263	795	186	715	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P33336|SKN1_YEAST	1.09e-310	263	795	225	767	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P87023|KRE6_CANAX	2.23e-272	275	789	223	739	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|P87024|SKN1_CANAX	6.55e-271	275	789	226	736	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|O13941|YEPB_SCHPO	9.41e-172	261	792	98	628	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000056	0.000000

TMHMM  Annotations     

Start	End
331	353