CAZyme Information: SPSK_01646-t39_1-p1

Basic Information

• Species: Sporothrix schenckii
• Lineage: Ascomycota; Sordariomycetes; ; Ophiostomataceae; Sporothrix; Sporothrix schenckii
• CAZyme ID: SPSK_01646-t39_1-p1
• CAZy Family: AA9
• CAZyme Description: concanavalin A-like lectin/glucanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
502	AXCR01000005|CGC10	53520.01	7.0574

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Sschenckii1099-18	10434	1397361	141	10293

• Gene Location: location=AXCR01000005:1245847-1247523(+)

Full Sequence      

MLPSVREPSPSSSSSKALAWITVAAWASLSWHGVEAANTPSTSPYPLTTDANCTCYHTNS
STANYFSHHSFFDFRNLSEYAHTPPVLKDAPSNINAPATNAFFSSDRWTNMWMAQNWDNG
ALMKANNSDVTGSDATLFMVNSPNNIYIEHNSDHVGNLSSRAAGAHHRTLHARDIPTYMV
MRTARLNAFQTSAEIESSSQGYKYLSVRMYARTVGSPGAITAMFTYRAGANNNLNLVQES
DLEIRTRDPPTRVQFTNQPSWNADGDIPQATRNVTMPGGNRWNDWAHYRMDWTPGATSWY
VDGQLASTITFQAPRDPSQVMFNSWSDGGSWSGNMSADGAAFLQIQWIEMVFNNTDAAYM
PKPGGCSNMCSIDETPVTGTPVLVSSGGQRGQGGNGGQGGNGGNRGNGGNGGNGGNGGNN
GNGGNRGNGGNNGNGGNRGNGGNNGNNGNNGNNGNNGNSGNHYQNCQSAKYGQCAGKTWN
GCGGCAAGTTCRFQNDYYSQCL

Enzyme Prediction     

No EC number prediction in SPSK_01646-t39_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	110	350	5.5e-87	0.9907407407407407

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185683	Glyco_hydrolase_16	4.65e-46	102	351	3	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
395595	CBM_1	3.27e-09	470	498	1	29	Fungal cellulose binding domain.
185684	GH16_lichenase	3.34e-09	191	348	56	208	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
225182	BglS	8.17e-08	187	378	100	290	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
197593	fCBD	8.38e-08	469	502	1	34	Fungal-type cellulose-binding domain. Small four-cysteine cellulose-binding domain of fungi

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEO63760.1|CBM1|GH16_22	1.04e-145	45	502	27	398
AEO55749.1|GH16_22	1.04e-116	46	387	35	385
AEO69403.1|GH16_22	2.15e-106	33	388	16	348
AEO57601.1|GH16_22	3.44e-105	47	388	38	361
CAP68982.1|GH16_22	4.99e-99	46	391	21	343

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3O5S_A	4.06e-12	179	348	73	233	Crystal Structure of the endo-beta-1,3-1,4 glucanase from Bacillus subtilis (strain 168) [Bacillus subtilis]
1GBG_A	5.11e-12	187	348	57	209	Bacillus Licheniformis Beta-Glucanase [Bacillus licheniformis]
1CPM_A	9.35e-12	193	359	4	162	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1MAC_A	3.04e-11	181	353	49	212	Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]
1AJK_A	3.14e-11	218	359	3	137	Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase Cpa16m-84 [Paenibacillus macerans],1AJK_B Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase Cpa16m-84 [Paenibacillus macerans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P04957|GUB_BACSU	2.21e-11	179	348	77	237	Beta-glucanase OS=Bacillus subtilis (strain 168) OX=224308 GN=bglS PE=1 SV=2
sp|P27051|GUB_BACLI	1.00e-10	187	348	86	238	Beta-glucanase OS=Bacillus licheniformis OX=1402 GN=bg1 PE=1 SV=1
sp|P45797|GUB_PAEPO	1.71e-10	187	353	81	238	Beta-glucanase OS=Paenibacillus polymyxa OX=1406 GN=gluB PE=3 SV=1
sp|P07980|GUB_BACAM	1.73e-10	179	348	74	234	Beta-glucanase OS=Bacillus amyloliquefaciens OX=1390 GN=bglA PE=3 SV=1
sp|P23904|GUB_PAEMA	2.27e-10	181	353	74	237	Beta-glucanase OS=Paenibacillus macerans OX=44252 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000408	0.999548	CS pos: 36-37. Pr: 0.9714

TMHMM  Annotations     

There is no transmembrane helices in SPSK_01646-t39_1-p1.