CAZyme Information: SPSK_01368-t39_1-p1

Basic Information

• Species: Sporothrix schenckii
• Lineage: Ascomycota; Sordariomycetes; ; Ophiostomataceae; Sporothrix; Sporothrix schenckii
• CAZyme ID: SPSK_01368-t39_1-p1
• CAZy Family: AA7
• CAZyme Description: sterol 3beta-glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1836	AXCR01000005|CGC12	199939.04	6.6594

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Sschenckii1099-18	10434	1397361	141	10293

• Gene Location: location=AXCR01000005:2139280-2144790(-)

Full Sequence      

MAPPRDPAAPTAASSTTPTPTPTQTPPLSTAPSDASNRNTNIDTTTSSTATLQAPPVAAS
ASSSPGPSRTVAGTASPTTPSTNRNDHQRKSHSHSLRLSSRLSAQSPSTSALASALASSP
TRLSNTSPRQSEDDAGRHKPKKLVKRRHDGGSQRTPKAMDFPERLKQSDDGGEEEVIRPV
VSGNGAFYNMNQSVFNMLAAAGSNIDFNNRFGDHSSEEDDEDGGDAEGGDGDVAQRRHFS
SDGAATTVLPAAGSQKKSGKKPAKDVSQSTIFTRNTSSTGKDGKDGKDSGRRSRFSDSKL
MRSLPSLKQKLSSSKRRSSSGLSSKSSKHASAGGTSDSRILEEDEDADAEAESSHTPSST
QTRLPRGSGATSDVDVAVGSARGSNHSPSIQVTRAERSNSVRSIPVMSRMLEAKELASLR
PSFDIDRDRKSSDKDKGDGVDVEDGGPSALSRKLMEIFEFDKPEEVIEEYPCWLLQSVLL
QGYMYITSRHICFYAYLPKKANEVAKSGYLAKSGKRNPKYNRYWFRLKGDVFSYYRDAKN
LYFPHGQIDLRYGISATVSDQDKDGVHFQVVTHHRTYFFRADSAASAKEWVKSLQRVIFR
SHNDGDSVKISLPIENVIDIEENQAMVEFTETCKIRVIDNDETFAIDEYFFSFFDYGKDA
VRVLKILIDANNVNSNDNNNNNNADSDNQSGQGTPSRAYGRRLDLPSAGQGMDDRVSQDS
TGSVAFGAGQERMSMSKPPSKLQDSVRATLLSIPPTAASSPRASGEIPRTSFDALRSSFS
PTRRSLDTSQVLRANPPRRSFSDRIKGAAGDTVQRKISQRSTSAAQPQSSDPQDSTDSFM
QSSVEDPSQISFSNMAASSSDDPSASQILHGSDVFQSPTFDAGSPHSAGPPPSTSRPAAA
AAAAAKPPPHHSATTGNIRKFKGAASDEAGENEEGLAQNLARLGTYPLQRAGAYAGWLNR
QSRRMSTMLATESMGYVEKVSGMWKGGRQHYDDASLTPHADAGMPDGEPGMSVADAIVSN
EHFREHFALPDTEKLEATYFGSIMRVLPLYGKIYVSQRYFCFRSLVPGTRTKLILPLKDI
ENVVKEKGFRYTYAGLVVVIRGHEELFFEFHQAELRDDCAITLLHLLDRFRSAKESGVLS
DQDKADADVALAERDALKEAREEAAARMDDHAETPAESTAAAVATAKAVAASSEAKAERL
NRKILRQSALMSEAPTILFDDPKASFLNFKPAKPLRITCLTIGSRGDVQPYIALGRGLLA
EGHHVRIATHPEFEGWIRSHGLDYGRVAGDPGELMRICIENGTFTIQFLREANSKMRTWL
DELLSTAWTACQGADLLIESPSAMAGIHIAEKLQIPYFRAFTMPWTRTRAYPHAFLMPER
RMGGAYNQFSYAMFENIFWRATAGQVNRWRNKTLGLRNTSLEKLQINKVPFLYNFSPCVV
PPPLDYSDWIRVTGYWFLDEGTNIKKANNNNNNNNNNNNNKSSSEPEGKEVAEAKEPAWT
PPQDLLDFIAKARKDRKKIVYVGFGSIVVADPAKMTQEVIDAVHKADVRCILSKGWSDRL
VAKKDDKTEAVAEAEAEAGAEAGAEAATGRVMHADPPEDVLPAEIFPIKSAPHDWLFAQI
DAAAHHGGSGTTGASLRAGIPTIIRPFFGDQFFFGMRVEDLGVGLCLKKWGAMSFARALW
EATNSERMIRKAAVLGEQIREENGVDTAIQSIYRDMEYAKSLVREKAAKSGMLRSGGGTE
RADRNDRGHGKHINADSDEEAYDDDDVDDDDEPEESWTFVGDDDPMQQSVILTPDAGRAS
GDDGVVQPASSGAGGSSGGYKALGSRILGIATAQKQ

Enzyme Prediction     

EC	2.4.1.173:13	2.4.1.173:13

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT1	1237	1712	7.3e-55	0.9738219895287958

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340817	GT1_Gtf-like	8.86e-73	1235	1712	1	401	UDP-glycosyltransferases and similar proteins. This family includes the Gtfs, a group of homologous glycosyltransferases involved in the final stages of the biosynthesis of antibiotics vancomycin and related chloroeremomycin. Gtfs transfer sugar moieties from an activated NDP-sugar donor to the oxidatively cross-linked heptapeptide core of vancomycin group antibiotics. The core structure is important for the bioactivity of the antibiotics.
275402	PH-GRAM1_AGT26	1.49e-56	484	598	1	116	Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, repeat 1. ATG26 (also called UGT51/UDP-glycosyltransferase 51), a member of the glycosyltransferase 28 family, resulting in the biosynthesis of sterol glucoside. ATG26 in decane metabolism and autophagy. There are 32 known autophagy-related (ATG) proteins, 17 are components of the core autophagic machinery essential for all autophagy-related pathways and 15 are the additional components required only for certain pathways or species. The core autophagic machinery includes 1) the ATG9 cycling system (ATG1, ATG2, ATG9, ATG13, ATG18, and ATG27), 2) the phosphatidylinositol 3-kinase complex (ATG6/VPS30, ATG14, VPS15, and ATG34), and 3) the ubiquitin-like protein system (ATG3, ATG4, ATG5, ATG7, ATG8, ATG10, ATG12, and ATG16). Less is known about how the core machinery is adapted or modulated with additional components to accommodate the nonselective sequestration of bulk cytosol (autophagosome formation) or selective sequestration of specific cargos (Cvt vesicle, pexophagosome, or bacteria-containing autophagosome formation). The pexophagosome-specific additions include the ATG30-ATG11-ATG17 receptor-adaptors complex, the coiled-coil protein ATG25, and the sterol glucosyltransferase ATG26. ATG26 is necessary for the degradation of medium peroxisomes. It contains 2 GRAM domains and a single PH domain. PH domains are only found in eukaryotes. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. PH domains also have diverse functions. They are often involved in targeting proteins to the plasma membrane, but few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.
275403	PH-GRAM2_AGT26	1.33e-47	1032	1124	1	93	Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, repeat 2. ATG26 (also called UGT51/UDP-glycosyltransferase 51), a member of the glycosyltransferase 28 family, resulting in the biosynthesis of sterol glucoside. ATG26 in decane metabolism and autophagy. There are 32 known autophagy-related (ATG) proteins, 17 are components of the core autophagic machinery essential for all autophagy-related pathways and 15 are the additional components required only for certain pathways or species. The core autophagic machinery includes 1) the ATG9 cycling system (ATG1, ATG2, ATG9, ATG13, ATG18, and ATG27), 2) the phosphatidylinositol 3-kinase complex (ATG6/VPS30, ATG14, VPS15, and ATG34), and 3) the ubiquitin-like protein system (ATG3, ATG4, ATG5, ATG7, ATG8, ATG10, ATG12, and ATG16). Less is known about how the core machinery is adapted or modulated with additional components to accommodate the nonselective sequestration of bulk cytosol (autophagosome formation) or selective sequestration of specific cargos (Cvt vesicle, pexophagosome, or bacteria-containing autophagosome formation). The pexophagosome-specific additions include the ATG30-ATG11-ATG17 receptor-adaptors complex, the coiled-coil protein ATG25, and the sterol glucosyltransferase ATG26. ATG26 is necessary for the degradation of medium peroxisomes. It contains 2 GRAM domains and a single PH domain. PH domains are only found in eukaryotes. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. PH domains also have diverse functions. They are often involved in targeting proteins to the plasma membrane, but few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.
224732	YjiC	2.48e-40	1236	1712	3	395	UDP:flavonoid glycosyltransferase YjiC, YdhE family [Carbohydrate transport and metabolism].
397255	Glyco_transf_28	2.07e-30	1237	1372	1	139	Glycosyltransferase family 28 N-terminal domain. The glycosyltransferase family 28 includes monogalactosyldiacylglycerol synthase (EC 2.4.1.46) and UDP-N-acetylglucosamine transferase (EC 2.4.1.-). This N-terminal domain contains the acceptor binding site and likely membrane association site. This family also contains a large number of proteins that probably have quite distinct activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBZ60676.1|GT1	0.0	124	1756	56	1495
UNI15418.1|GT1	0.0	133	1733	26	1388
AEO60294.1|GT1	0.0	141	1730	59	1438
BAH60889.1|GT1	0.0	132	1730	24	1412
UPK90680.1|GT1	0.0	132	1736	16	1384

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XVM_A	1.15e-161	1217	1722	2	445	Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) [Saccharomyces cerevisiae S288C],5XVM_B Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) [Saccharomyces cerevisiae S288C]
5GL5_A	2.29e-161	1217	1722	22	465	Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c): UDPG complex [Saccharomyces cerevisiae S288C],5GL5_B Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c): UDPG complex [Saccharomyces cerevisiae S288C]
1IIR_A	1.25e-17	1235	1696	1	380	Crystal Structure of UDP-glucosyltransferase GtfB [Amycolatopsis orientalis]
1RRV_A	2.86e-08	1235	1665	1	348	X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin. [Amycolatopsis orientalis],1RRV_B X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin. [Amycolatopsis orientalis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WID6|ATG26_ASPFU	0.0	133	1730	6	1333	Sterol 3-beta-glucosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=atg26 PE=3 SV=2
sp|Q0CKU4|ATG26_ASPTN	0.0	397	1730	184	1329	Sterol 3-beta-glucosyltransferase OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=atg26 PE=3 SV=1
sp|Q0UY53|ATG26_PHANO	0.0	411	1725	192	1379	Sterol 3-beta-glucosyltransferase OS=Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) OX=321614 GN=ATG26 PE=3 SV=1
sp|Q2U0C3|ATG26_ASPOR	0.0	394	1730	180	1323	Sterol 3-beta-glucosyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=atg26 PE=3 SV=2
sp|Q7S1I0|ATG26_NEUCR	0.0	141	1729	71	1446	Sterol 3-beta-glucosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=apg-12 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000056	0.000012

TMHMM  Annotations     

There is no transmembrane helices in SPSK_01368-t39_1-p1.