CAZyme Information: SPRG_15913-t26_1-p1

Basic Information

• Species: Saprolegnia parasitica
• Lineage: Oomycota; NA; ; Saprolegniaceae; Saprolegnia; Saprolegnia parasitica
• CAZyme ID: SPRG_15913-t26_1-p1
• CAZy Family: GT20
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
793	KK583392|CGC1	88335.10	6.0071

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SparasiticaCBS223-65	20435	695850	314	20121

• Gene Location: location=KK583392:11307-13796(-)

Full Sequence      

MWWRLFLVLGAAMAEVQDAPTKSGVKTWVDVETPEWAMTKMTSRGHEYDLVMSDEFNNEN
RNFTAGMDHLWTALNLPDGVNSAIGYYSPRNTYTSNGKFLIRVDEGTVPIQYWDQYSATP
GWVNKNMYYTGAMVQTWNKFCLQGGLIEVRLQLPGALDAASGNPKYPPYQRLVESDRGFY
PTWPGIWLMGNLGRALFSASTRRMWPWTYNECDPRYKDMQRISACNATPGHGLNPFQGRG
SPEIDILEGGGNEISSSIQIGPGMPFDYRLYNADDGGCFYDKKCKYTGANMANVPSAAFK
RQHWYQGLRYAANNKCAPNASYGQQFNSVYQSVMAKKITTNTCFSSPGDQQVIPASCDVN
GDLGLIDGVGPTHWGVNEKSTCFAQANGYKGVYLCDPDNQYYRCEFARPDGRAKTNQMTP
FNYQMDALSANWFTTQEAYRGYMNYSLEWVLGPNGHIRWALDDQVLFEIPSQSILNVPQG
GNETNPIKLPIEEPMYLIFNVATAPAWGTMPPNAGYGPCRGNQTDPGWLWKNKTNRATVV
AETKICDSFPMYLSVDYIRVWQDLRGNEENASHIGCDPASHPTSQWILDNIGEYSDANNS
VIDVAGGATCNSDDDCTRASMMTGICVKNRCQCTTLWGGPRCTWVVDIEPNWAYRAQASA
KGFGPTVPITVSLLALVLVSIAFIQYRQSKGHFMFFGKVRGPPTPVTRASSVANDDVMAT
EDRARALLNQSSLENASFMDSHGHVFDSNHGMDIGHTRYELETTPSEGHHVRFTDKAFAI
DANGRKTAPSTLM

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	54	306	8.9e-66	0.7239263803680982

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	7.90e-59	20	606	88	488	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	3.46e-42	50	562	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	2.87e-08	29	263	30	215	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	4.58e-07	53	263	2	138	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185693	GH16_laminarinase_like	4.79e-05	445	561	163	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG55703.1|GH16_2	2.11e-237	7	679	12	712
UIZ28149.1|GH16	3.94e-220	7	673	9	686
UIZ27810.1|GH16	2.76e-214	18	677	64	723
UIZ28151.1|GH16	2.97e-214	14	680	25	695
UIZ28152.1|GH16	2.33e-212	21	689	20	688

PDB Hits     

SPRG_15913-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P33336|SKN1_YEAST	9.59e-33	27	262	357	574	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P32486|KRE6_YEAST	1.78e-31	20	606	297	699	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|O13941|YEPB_SCHPO	3.40e-31	29	606	219	615	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1
sp|P87024|SKN1_CANAX	9.98e-30	20	310	326	608	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P87023|KRE6_CANAX	2.11e-27	29	598	332	721	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000285	0.999702	CS pos: 18-19. Pr: 0.7272

TMHMM  Annotations     

Start	End
662	684