CAZyme Information: SPRG_06920-t26_1-p1

Basic Information

• Species: Saprolegnia parasitica
• Lineage: Oomycota; NA; ; Saprolegniaceae; Saprolegnia; Saprolegnia parasitica
• CAZyme ID: SPRG_06920-t26_1-p1
• CAZy Family: GH17
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1518	KK583216|CGC5	169355.21	5.7626

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SparasiticaCBS223-65	20435	695850	314	20121

• Gene Location: location=KK583216:395033-400241(-)

Full Sequence      

MSSGCARRWLAAAMALVLAMTVAASRKVDVDLTTTFLSSPVYPVLETSAFLAEVDPPLFF
RYVDAIDMRAKAIQQKDSINYTALALDAAHDVLPPDATIAKLLPFVLQTRAQSPKIEMFH
QLAIESAYKGCGLAYEAASAWVVIYHETGCATRVVCAASDWDNALLTPSTDTSCAASGEH
DFELHVDHVFDKDVANAPTVLLYGTLGTSSFRAFHSKLAPLAEAKAVRYIVRHAPQNNVE
PVHLQGYGVSLHIKNMEYKSFDDSKTPHSKHTPTDDDEDEFIVSVLMKKGAETGPALRAY
QLEFEDFSSVNATENNEDAPWQLNHLGYLAASDILASTDPLARLQQLAQNFPKYASGLAL
TTEPIPSERIAAMATARRTVATQQLLNKIVVNGMVHDLSDVTFNPFDFVKLLAGEVQQGD
ALRALELDDTTLTAVHSAMTTLSTQASEVRVRVRGPVDGTAPLYLNAIESDEMTAEWPTD
VSVLAEPAWNLIFLRRVMYEVILVLDPTTKQAANVLQQIHLLILRGAPVQFGVLWTSPEL
LALAPEDRETYVPATDDKAPASAYHVTKLYLAARDHGHEAAKQFLDGIVELGGGVTVKEA
LLIYVQAVTHRLDRGSALEAARDLLLSVSQDDGVWAMTDVVLASQLPLDSYVFNGVLRRD
LNLQEGLMAHFGRDQPLYQELARAGKFSDDADLLEELYAHEGFYPVYSSWLDPSQTVPVL
DLHLTSSVWDDVTYLHMPHTATKPKRQSLLLMVDLDTPAGARSAYNALKTTMETPDVRLS
LVHLGTSRSSIGSRVSYMLHALSDLDDARYLAIVLEILRFLSKAKSESDIVLHAKLLVQV
HLDASPDETRLHELLQWLEETPAYTHPLPSLLSMTSPTLLINGRPLSLDDNMTLLPSLLS
VLLQWEGEHRSKAVARAFSAPTKRDALSVEAAATMSQRLQTLLAVVDAYLATPRTGPVFP
TLDRHTTFATSAASRSLLDVIAYLDPLSEATQRASVVLRMLRDVLGAKITLVLTPTPSYT
EFPLKRFYRYAWGSAPSFRVLPRPPVLTMNVETPELFNVQMVASDTDVDNIQGDATARYA
VQSLLVYGQCIDRTMAYHPSPPNGLQLILERTAGADVLHRDTVVMKNLGYFQLQATPGVW
RLELAKGRASELYEMVDQAASDHARVVVYDFSSDITQLDVKKRPGFEYARLLEEPEDQPA
AKKKEEDEATQSYWKSLVSWGTKQPAKDAVATRRGETIHVFSVATGHLYERMLKLMMLSV
LKRTNNPVTFWLLENFLSPDFKKSVPALQAEFGMDIRLVTYKWPNWLRRQTEKQRIIWGY
KILFLDVLFPLGVQKIIYVDADQVVRADLKELWHMDLHGKPYAYTPFCDSRNVGFQFWRQ
GYWKDHLRGKPYHISALYVVDLVKFRRMAAGDTLRAVYDQLSADPNSLSNLDQDLPNYAQ
HQIPIHSLPQEWLWCESWCSDESKASAKTIDLCNNPKHKEPKLEMAKRVISGEYFKESWL
QLDDEVKAAEAAYATRHA

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1238	1483	4.7e-124	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1238	1508	1	267	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	3.02e-165	1238	1483	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
132996	Glyco_transf_8	7.86e-57	1238	1478	1	243	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
408199	Thioredoxin_12	1.94e-30	44	240	5	183	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
408201	Thioredoxin_14	1.63e-25	458	711	9	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG56125.1|GT24	0.0	1	1513	1	1505
AIG55512.1|GT24	0.0	14	1514	9	1516
UIZ20865.1|GT24	0.0	12	1518	12	1665
CCA14500.1|GT24	0.0	25	1513	35	1665
AIZ94008.1|GT24	4.41e-203	187	1507	216	1595

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5NV4_A	8.73e-157	22	1516	5	1452	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5MU1_A	3.18e-156	22	1516	5	1452	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	8.38e-156	22	1516	5	1452	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	4.14e-139	965	1516	643	1218	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	1.92e-129	25	1445	1	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0WL80|UGGG_ARATH	7.02e-200	99	1507	101	1566	UDP-glucose:glycoprotein glucosyltransferase OS=Arabidopsis thaliana OX=3702 GN=UGGT PE=1 SV=1
sp|Q09332|UGGG_DROME	2.80e-190	39	1507	41	1505	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	3.08e-186	7	1513	26	1527	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	4.33e-183	10	1513	29	1527	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	4.70e-183	25	1513	44	1527	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.001471	0.998496	CS pos: 24-25. Pr: 0.9608

TMHMM  Annotations     

There is no transmembrane helices in SPRG_06920-t26_1-p1.