CAZyme Information: SPRG_06217-t26_1-p1

Basic Information

• Species: Saprolegnia parasitica
• Lineage: Oomycota; NA; ; Saprolegniaceae; Saprolegnia; Saprolegnia parasitica
• CAZyme ID: SPRG_06217-t26_1-p1
• CAZy Family: GH17
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1017	KK583212|CGC3	110022.71	6.6110

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SparasiticaCBS223-65	20435	695850	314	20121

• Gene Location: location=KK583212:61698-64799(-)

Full Sequence      

MKVLHLLSLLSTLAAAVDYDAQAEAIVAKMTTDEWIGQMTQLDLSYIVDVNGQLIESKVR
ECAKLRVGSFLNGQMNVTAWRSLLGRIQDIYKEEGAPPMLFGLDSIHGASYTFGAALSPQ
QTNKGSSFNRDLVRKSAYVTARDSAASGVNWIFAPNGDVVVHKRWSRIFESYGEDPYLVS
EMIAEAVTGIQSVPGVAANFKHFTAYGATSTGDDRGGAFPTEYELLNYYSPPFISAVNAG
ALSGMSSYVVVNNVPMVSNQKLTVDLLRSDMKFDGLLVTDWQDIYNLLHHNVGVSTRQDA
IAMSLNQASIDMSMVPDDPDFIYLSKNLVAAGKVPASRYRAGAKRVIKMKLQLDMFKTPL
PGADLVSQVGSDADRAEALELARESIVLLKNKDSVLPLSGRPRLFLTGPSIDNIGYLCGA
WTWAWQGVSGNDRFPKGKSILKGFQDLYGADANKIQMLQGVDITGAATDSLAEAKAKAKN
AEYTIIAVGEAPSTEMSGNIADAALPAGQLQYIREIASTGTKVILVLVEARPRILKGVAD
VVHAVIDAMLPCEAGGQAIAEIVLGFTNPSGRLSMTYPKDTTYANMVTPYFNRKNGVCVA
DSCVAEWDFGAGLSYTTFSYTNFALSSNQVSISGAITASVTVTNTGAVQGKEAVLLFVSQ
QTRASGTPEIKMLKKFTKVDLAPGQSTQVTFSLSVADWGIYAGPIGSGLTKTAEAGTYFV
AFKPETVCNAANVGSLCQAFTYDTLSTPTPKPSADTVLSLYTKTGKIIGTDQWNVWVQTP
GASPSRSQQLVYDSVTKQLRDKSLGRCFDVYPDASAPIKYRLHMYACDASNGNQKWTLNA
ATHQVIHATHPNICLDVDPSDTTGAVQVWTCVSGNPNQFFSRGIPERVRLSSVLNNRVFT
VASTAARAAVTFSAPPTSLPNWSFDFASGLVRVDGSTQCLDAYQAQNGGAVHLWTCDASN
GNQKWIYEPTTGQLRHGTFQSFCLDMQSDVGATPYLWTCHNPNNYWFKFQTFKYEPL

Enzyme Prediction     

EC	3.2.1.21:1	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	98	315	2.7e-54	0.9537037037037037

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185053	PRK15098	2.77e-92	18	720	33	748	beta-glucosidase BglX.
224389	BglX	2.38e-65	30	422	1	372	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
395747	Glyco_hydro_3	6.11e-49	31	347	1	314	Glycosyl hydrolase family 3 N terminal domain.
178629	PLN03080	2.17e-45	95	697	80	752	Probable beta-xylosidase; Provisional
396478	Glyco_hydro_3_C	1.53e-31	386	615	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG56285.1|CBM13|GH3	0.0	14	1010	16	1036
AIG55915.1|CBM13|GH3	0.0	14	1017	17	1027
AIG55751.1|CBM13|GH3	0.0	7	1017	9	1023
AIG56284.1|GH3	3.01e-295	18	742	19	740
AIG55594.1|GH3	2.18e-240	1	569	1	568

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5JP0_A	4.45e-113	29	697	23	718	Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus],5JP0_B Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus]
5XXL_A	4.60e-76	18	694	9	713	Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	2.23e-75	18	694	9	713	Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
4ZOA_A	3.31e-69	22	727	5	712	Crystal Structure of beta-glucosidase from Listeria innocua in complex with isofagomine [Listeria innocua Clip11262],4ZOA_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with isofagomine [Listeria innocua Clip11262],4ZOB_A Crystal Structure of beta-glucosidase from Listeria innocua in complex with gluconolactone [Listeria innocua Clip11262],4ZOB_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with gluconolactone [Listeria innocua Clip11262],4ZOD_A Crystal Structure of beta-glucosidase from Listeria innocua in complex with glucose [Listeria innocua Clip11262],4ZOD_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with glucose [Listeria innocua Clip11262],4ZOE_A Crystal Structure of beta-glucosidase from Listeria innocua [Listeria innocua Clip11262],4ZOE_B Crystal Structure of beta-glucosidase from Listeria innocua [Listeria innocua Clip11262]
4ZO6_A	3.99e-68	22	727	5	712	Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with cellobiose [Listeria innocua Clip11262],4ZO6_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with cellobiose [Listeria innocua Clip11262],4ZO7_A Crystal structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with gentiobiose [Listeria innocua Clip11262],4ZO7_B Crystal structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with gentiobiose [Listeria innocua Clip11262],4ZO8_A Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorose [Listeria innocua Clip11262],4ZO8_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorose [Listeria innocua Clip11262],4ZO9_A Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with laminaribiose [Listeria innocua Clip11262],4ZO9_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with laminaribiose [Listeria innocua Clip11262],4ZOC_A Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorotriose [Listeria innocua Clip11262],4ZOC_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorotriose [Listeria innocua Clip11262]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q23892|GLUA_DICDI	4.92e-134	20	720	77	809	Lysosomal beta glucosidase OS=Dictyostelium discoideum OX=44689 GN=gluA PE=1 SV=2
sp|A7LXU3|BGH3B_BACO1	3.24e-112	29	697	45	740	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1
sp|P33363|BGLX_ECOLI	1.72e-71	20	695	35	727	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
sp|Q56078|BGLX_SALTY	3.22e-71	20	695	35	727	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
sp|T2KMH0|PLH24_FORAG	5.14e-68	95	721	64	705	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000277	0.999717	CS pos: 16-17. Pr: 0.9252

TMHMM  Annotations     

There is no transmembrane helices in SPRG_06217-t26_1-p1.