CAZyme Information: SPRG_06216-t26_1-p1

Basic Information

• Species: Saprolegnia parasitica
• Lineage: Oomycota; NA; ; Saprolegniaceae; Saprolegnia; Saprolegnia parasitica
• CAZyme ID: SPRG_06216-t26_1-p1
• CAZy Family: GH17
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1011	KK583212|CGC2	107099.16	6.7966

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SparasiticaCBS223-65	20435	695850	314	20121

• Gene Location: location=KK583212:58348-61568(+)

Full Sequence      

MKLYLPLAVVAAVAVAQDFDAQAEAIVAKMTPAQWIGQMAQANIGVILGSDGQLSVNAVT
QCAKAGVGSFLNGAMPAAKWRAMITQIQSIYKANNAPPALYGLDSVHGANYVNDAILSPQ
QINKGATFNAKIVQDSAFVTARDTSAGGVNWVFAPGLDVTNHKRWSRVYETYGEDPYLAS
ELGVAAVNGIQSVPGVAAAVKHFIGYGATSTGDDRAPAYLDDYQVLNYHAPPFIAAIQNA
NALSVMASYVSVNGVPMVANSRLTNDLLRSDIGFKGLLLTDWEDIYNLNYVHKLVTNNQD
AVAMSMSKSSVDMSMIPNDLSFIGHAQNLLNAGTIAASRYRASAKRIIKMKLQLGLFETP
VPGANNVNLVGSNADRQASLELARESIVLPVRSNPNLFVTGPAYDNIGYLCGGWTWEWQG
VSGNAKFPGSQSVLAAFQSLVADKGGKVTSLQGVDINGGTTDSLADAKAKAAAAEYTVIV
VGEAPYAEMVGNIPDAALPAGQLQYIRDIASTGTKVILVLVEGRPRLLNGVADVVHAVID
AMLPCHMGGQAIAEIILGVTNPSGRLPLTYPKDATKDNMVTPYFHRKNGVCVLTGAACPA
EWEFGAGLSYTTFEYSNFALSANQLNTSSESLTASVVVTNTGAVAGKEAVLVFLSQGARA
SGTPETKMLKKFTKVSLAPGQSTTVTFPIGFNDFAIYNGGIGSGLLKSAEAGAYYVGVKP
ETVCDANTIGPLCKSFKYGAVAATVPLSLYAKTTGKIVGTTDWDTFMYSPPTGATPANQQ
LEYLPDTRQLRVKSNGKCLDVYPNSAVAAGYTLHLWTCDAANGNQRWRINAAGHQIAHAT
HNNVCLDADPTDGQSRLQVWSCAPVGTNPNQFFGLTSVTAEPATLTSLSGLRFGANVGSG
SVGFGSGASVWSFNFMTGQIVAQGSNQCLDAYQAQNGGAVHLWTCDASNGNQKWIYEPTT
GQLRHGTFKSFCLDMQSDAGATPYVWTCHDPNNYWFKFQTFKYQNTAVALP

Enzyme Prediction     

EC	3.2.1.21:1	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	97	315	3.5e-58	0.9537037037037037
CBM13	767	875	8.4e-18	0.5478723404255319

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185053	PRK15098	9.17e-87	17	716	32	748	beta-glucosidase BglX.
224389	BglX	2.12e-62	30	415	1	372	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
395747	Glyco_hydro_3	2.73e-47	36	348	6	314	Glycosyl hydrolase family 3 N terminal domain.
178629	PLN03080	1.97e-42	99	689	90	742	Probable beta-xylosidase; Provisional
396478	Glyco_hydro_3_C	3.32e-23	388	610	10	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG56285.1|CBM13|GH3	0.0	1	1005	1	1042
AIG55751.1|CBM13|GH3	0.0	1	1004	3	1021
AIG55915.1|CBM13|GH3	0.0	1	1005	1	1026
AIG56284.1|GH3	0.0	18	751	19	753
AIG55594.1|GH3	2.33e-293	15	562	14	568

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5JP0_A	1.93e-115	29	695	23	720	Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus],5JP0_B Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus]
5M6G_A	9.15e-65	98	570	123	587	Crystal structure Glucan 1,4-beta-glucosidase from Saccharopolyspora erythraea [Saccharopolyspora erythraea D]
5XXL_A	1.60e-63	17	698	8	721	Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	7.39e-63	17	698	8	721	Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
3RRX_A	1.19e-60	20	565	23	571	Crystal Structure of Q683A mutant of Exo-1,3/1,4-beta-glucanase (ExoP) from Pseudoalteromonas sp. BB1 [Pseudoalteromonas sp. BB1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q23892|GLUA_DICDI	1.12e-127	20	695	77	792	Lysosomal beta glucosidase OS=Dictyostelium discoideum OX=44689 GN=gluA PE=1 SV=2
sp|A7LXU3|BGH3B_BACO1	1.41e-114	29	695	45	742	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1
sp|T2KMH0|PLH24_FORAG	3.56e-68	97	725	66	713	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
sp|P33363|BGLX_ECOLI	1.85e-67	20	698	35	734	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
sp|Q56078|BGLX_SALTY	1.03e-65	20	698	35	734	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000248	0.999740	CS pos: 16-17. Pr: 0.9134

TMHMM  Annotations     

There is no transmembrane helices in SPRG_06216-t26_1-p1.