CAZyme Information: SPBR_06672-t41_1-p1

Basic Information

• Species: Sporothrix brasiliensis
• Lineage: Ascomycota; Sordariomycetes; ; Ophiostomataceae; Sporothrix; Sporothrix brasiliensis
• CAZyme ID: SPBR_06672-t41_1-p1
• CAZy Family: GH79
• CAZyme Description: dolichyl-diphosphooligosaccharide--protein glycosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
752	AWTV01000009|CGC27	83770.94	8.7073

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Sbrasiliensis5110	9231	1398154	140	9091

• Gene Location: location=AWTV01000009:6358621-6361095(+)

Full Sequence      

MASTSTTPNPIGPLGDAGKTRSLRSVLRVVILFLIAAASVSSRLFSVIRFESIIHEFDPW
FNFRATKYLVANGFYSFWDWFDDRTWHPLGRVTGGTLYPGLMVTSGAIYHALRALTIPVD
IRNVCVMLAPAFSGLTAYSAYLLTSEMTTSPSAGLLAAAFMGITPGYISRSVAGSYDNEA
IAIFLLVFTFYLWIKALKLGSMLWGALCALFYGYMVASWGGYAFITCLLPLHAFVLICMG
RYSTRLYVAYTTWYALGTLASMQIPFVGFLPVRTSEHMPALGIFGFLQLLAFLDYVRSVV
PTRQFQTFLLLSAFSVFSIGLLGLVIATTAGVIAPWSGRFYSLWDTGYAKIHIPIIASVS
EHQPTAWPAFFFDLNFLIWLFPAGVYLCFQTLADEHVFIVVYALFGSYFAGVMVRLMLTL
TPVVCVAAAITVSSLLDGYLNAKSPSPADVAAAEENADKKPSKGGLKSMSRPVVGVYSFL
SKSFVTGAVVIYLVMFVAHCTWVTSNAYSSPSVVLASKLPDGSQHIIDDYREAYQWLRQN
TPKDAKVMSWWDYGYQIGGMADRPTLVDNNTWNNTHIATVGKAMSSREEVSYPIMKAHEV
DYVLVVFGGLLGYSGDDINKFLWMVRIAEGIWPDEVKERDFFTARGEYRVDDEATDTMKN
SLMYKMSYYNYQALFPPGQAQDRVRGSRLPEKAPTLNTLEEAYTSENWIIRIYKVKDYDN
VGRDHGAVASFEQGHKKKRSTKRKGARVLRID

Enzyme Prediction     

EC	2.4.99.18:21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT66	27	671	9.5e-192	0.8484848484848485

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396873	STT3	1.71e-146	26	509	1	478	Oligosaccharyl transferase STT3 subunit. This family consists of the oligosaccharyl transferase STT3 subunit and related proteins. The STT3 subunit is part of the oligosaccharyl transferase (OTase) complex of proteins and is required for its activity. In eukaryotes, OTase transfers a lipid-linked core-oligosaccharide to selected asparagine residues in the ER. In the archaea STT3 occurs alone, rather than in an OTase complex, and is required for N-glycosylation of asparagines.
224206	Stt3	9.76e-111	21	723	13	737	Asparagine N-glycosylation enzyme, membrane subunit Stt3 [Posttranslational modification, protein turnover, chaperones].
275016	archaeo_STT3	2.36e-29	56	564	32	547	oligosaccharyl transferase, archaeosortase A system-associated. Members of this protein family occur, one to three members per genome, in the same species of Euryarchaeota as contain the predicted protein-sorting enzyme archaeosortase (TIGR04125) and its cognate protein-sorting signal PGF-CTERM (TIGR04126).
404170	PMT_2	0.002	127	241	26	135	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
396786	PMT	0.009	132	274	92	238	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEO65709.1|GT66	0.0	14	752	9	747
QLI73673.1|GT66	0.0	13	752	7	748
QBZ55568.1|GT66	0.0	14	752	9	750
CAE76502.1|GT66	0.0	14	752	9	749
AEO54301.1|GT66	0.0	9	752	4	750

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6C26_A	2.31e-317	24	751	13	718	The Cryo-EM structure of a eukaryotic oligosaccharyl transferase complex [Saccharomyces cerevisiae S288C],6EZN_F Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex [Saccharomyces cerevisiae S288C],7OCI_F Chain F, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 [Saccharomyces cerevisiae S288C]
6S7T_A	1.02e-283	8	720	53	777	Cryo-EM structure of human oligosaccharyltransferase complex OST-B [Homo sapiens]
6FTG_5	3.40e-259	25	722	16	701	Subtomogram average of OST-containing ribosome-translocon complexes from canine rough microsomal membranes [Canis lupus familiaris],6FTI_5 Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Programmed 80S Ribosome [Canis lupus familiaris]
6S7O_A	6.83e-259	25	722	16	701	Cryo-EM structure of human oligosaccharyltransferase complex OST-A [Homo sapiens]
6FTJ_5	3.27e-257	25	717	16	696	Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Non-programmed 80S Ribosome [Canis lupus familiaris]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O94335|STT3_SCHPO	0.0	27	738	24	738	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=stt3 PE=3 SV=1
sp|P39007|STT3_YEAST	1.19e-316	24	751	13	718	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=STT3 PE=1 SV=2
sp|Q3TDQ1|STT3B_MOUSE	2.06e-284	2	720	41	774	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Mus musculus OX=10090 GN=Stt3b PE=1 SV=2
sp|E2RG47|STT3B_CANLF	1.30e-283	8	720	53	777	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Canis lupus familiaris OX=9615 GN=STT3B PE=1 SV=1
sp|Q8TCJ2|STT3B_HUMAN	5.25e-283	8	720	53	777	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Homo sapiens OX=9606 GN=STT3B PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000003	0.000002

TMHMM  Annotations     

Start	End
26	48
90	112
124	142
152	174
181	203
218	240
247	269
279	301
308	330
367	389
396	418
474	496