CAZyme Information: SPBR_06577-t41_1-p1

Basic Information

• Species: Sporothrix brasiliensis
• Lineage: Ascomycota; Sordariomycetes; ; Ophiostomataceae; Sporothrix; Sporothrix brasiliensis
• CAZyme ID: SPBR_06577-t41_1-p1
• CAZy Family: GH76
• CAZyme Description: beta-mannosidase precursor

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
888	AWTV01000009|CGC6	98784.41	6.2113

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Sbrasiliensis5110	9231	1398154	140	9091

• Gene Location: location=AWTV01000009:3052715-3055532(-)

Full Sequence      

MAVQRVDIDQGWEFRQASSLNNATASSFLPVAQFPTVAHIDLLHHKLIPDPYIDDNELTC
LWVNDADWTYRTTLPATPLPTPQARAVLVFEGLDTVVDVFLGGTRILSSKNMHVEHRVDV
TDQLRAAGGPLSPELSLELQFRAAPAFSRQEKKRIGYKGNETDVHFGGPERLFLRKAQYH
WGWDWGPAVNTSGPWKPVYLEVYETRIDRLVVRQQVSDDLAAATISVQGRVETVAKAQEH
VHLHVSDPSGASLHSHRLQTAADGSFATTVAVDTPQLWYPHMYGDQPLYTVSASVALPDA
ASSQDSTITGTKTSRRIGLRRLRLLQHPLKEAAGTSFVFEVNNIRLFCGGSCWIPGDFML
PRMTRQRYRDWIQLARDGHQSMIRVWGGGIVESDDFYDLCDEAGMLVWQDFLFACGNYPA
SGDFLDSVRREAEQQVVRVGHHASLALWAGNNEDYMVAERWGWDLDMSDETGPWDQTNFP
ARAIYERVLPAVVETLGGDVPYWRSSPYGGAFSNDTTVGDTHIWDVWHGKMSAYQDYKSY
TSRFVSEFGFESCASVATMERGITVPAERHAQSRLFDIHDKGPGHARRYPMYMGENFRFR
MNPFRDFVYCTQFLQAEALRYAYNCWRRAFRGPGEEHCAGVLVWQLNDIWPGTSWALVDV
DRRPKPAYYITKRALAPAVVGMERTVTGAPSYITTGYPPDRHALDVWAVNGDLAARHATL
TLAAYNIHTGAPVPLPRGVAATRSVVLAPNQTTELVRRMAIPAADSTVVVAYLDGPAPTA
ATAAPPSRLARWVSWPEPLRLVRMSPALAVTATVSDAGDQVLLTANAPAKGVTLHVSPTD
GVADAVFDDNFVDLVPGETVAVGVHGLRGRAVEVRFLYDWELKEGFEL

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	687	7.6e-84	0.6170212765957447

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	4.61e-62	47	677	46	631	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407625	Ig_mannosidase	5.10e-10	806	879	2	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
395572	Glyco_hydro_2	3.47e-08	205	310	1	104	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
407658	Mannosidase_ig	1.07e-04	704	800	3	90	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UKZ74807.1|GH2	0.0	1	888	1	869
QYS95286.1|GH2	0.0	1	888	1	869
UKZ48272.1|GH2	0.0	1	888	1	861
QPC62730.1|GH2	0.0	3	888	2	865
CEI70352.1|GH2	0.0	3	887	2	864

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	1.84e-120	2	683	23	661	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
6BYE_A	3.46e-109	6	688	6	683	Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]
6BYC_A	3.54e-109	6	688	6	683	Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
2VJX_A	1.30e-108	8	680	11	656	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	1.36e-108	8	680	13	658	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|I2C092|MANBB_THETO	3.16e-235	6	877	7	852	Beta-mannosidase B OS=Thermothelomyces thermophilus OX=78579 GN=man9 PE=1 SV=1
sp|A1CGA8|MANBB_ASPCL	1.18e-190	2	868	3	832	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
sp|B8NW36|MANBB_ASPFN	3.20e-190	2	878	3	841	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1
sp|Q2TXB7|MANBB_ASPOR	4.52e-190	2	878	3	841	Beta-mannosidase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndB PE=3 SV=3
sp|A1D911|MANBB_NEOFI	5.76e-188	3	868	4	832	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000054	0.000000

TMHMM  Annotations     

There is no transmembrane helices in SPBR_06577-t41_1-p1.