CAZyme Information: SPBR_04032-t41_1-p1

Basic Information

• Species: Sporothrix brasiliensis
• Lineage: Ascomycota; Sordariomycetes; ; Ophiostomataceae; Sporothrix; Sporothrix brasiliensis
• CAZyme ID: SPBR_04032-t41_1-p1
• CAZy Family: GH18
• CAZyme Description: sterol 3beta-glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1808	AWTV01000005|CGC2	196983.09	6.6424

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Sbrasiliensis5110	9231	1398154	140	9091

• Gene Location: location=AWTV01000005:238141-243567(+)

Full Sequence      

MPPPRDPAAPTAASSTTPTPTPTQTPPLSTAPSSDASNRNTNTDTTTSSTATLQATPGPS
RTVTDTASPTTPSTNRNDHQRKSHSHSLRLPSRVSAQSPSTSALASALASSPTRLSNTSP
RQSEDDAGRHKPKKLVKRRHDGGSQRTPKAMDFPERLKQSEDGGEEEVIRPVVSGNGAFY
NMNQSVFNMLAAAGSNIDFNNRFGDHSSEEDDDDGGDAEGGDGDAAQRRHFGSDGAAMTV
LPAAGSHKKSGKKPAKDVSQSTIFTRNTSSSSTGKDGKDGKDGERRSRFSDSRLMRSLPS
LKQKLSSSKRRSSSGLSSKSSKHASAGGTGGSRILEEDEDADAEAESSHTPSSTQTRLPR
GSGATSDVDVAVGSARGSNHSPSIQVTRAERSNSVRSIPVMSRMLEAKELASLRPSFDID
RDRKAGDKDKGDVVDAEDGGPSALSRKLMEIFEFDKPEEVIEEYPCWLLQSVLLQGYMYI
TSRHICFYAYLPKKANEVAKSGYLAKSGKRNPKYNRYWFRLKGDVFSYYRDAKNLYFPHG
QIDLRYGISATVSDQDKDGVHFQVVTHHRTYFFRADSAASAKEWVKSLQRVIFRSHNDGD
SVKISLPIENVIDIEENQAMVEFTETCKIRVIDNDETFAIDEYFFSFFDYGKDAVRVLKI
LIDANNVNSNSNNNADSDNQSGQGTPSRAYGRRLDLPSAGQGMDDRVSQDSTGSVAFGAG
QERMSMSKPPSKLQDSVRATLLSIPPTAASSPRASGEIPRTSFDALRSSFSPTRRSLDTS
QVLRANPPRRSFSDRIKGASGEKVQRKVSLRSTSAAQPQSSDPQDSTDSFMQSSVEDPSQ
ISFSNMAASSSDDPSASQILHGSDVFQSPTVDAGSPHSAGPPPSTSRPAAAAAAAAAKPL
PRHSATTGDIRKFKGAASDEVGDNEEGLAQNLARLGTYPLQRAGAYAGWLNRQSRRMSTM
LATESMGYVEKVSGMWKGGRQHYDDASLTPHADAGIPDGEPGMSVADAIVSNEHFREHFA
LPDTEKLEATYFGSIMRVLPLYGKIYVSQRYFCFRSLVPGTRTKLILPLKDIENVVKEKG
FRYTYAGLVVVIRGHEELFFEFHQAELRDDCAITLLHLLDRFRSAKESGVLSDQDKAEAD
VALAERDALKEAREEAAARMDDHAETPAESTAAAVATAKAVAASSEAKAERLNRKILRQS
ALMSEAPTILFDDPKASFLNFKPAKPLRITCLTIGSRGDVQPYIALGRGLLAEGHHVRIA
THPEFEGWIRSHGLDYGRVAGDPGELMRICIENGTFTIQFLREANSKMRTWLDELLSTAW
TACQGADLLIESPSAMAGIHIAEKLQIPYFRAFTMPWTRTRAYPHAFLMPERRMGGAYNQ
FSYAMFENIFWRATAGQVNRWRNKTLGLRNTSLEKLQINKVPFLYNFSPCVVPPPLDYSD
WIRVTGYWFLDEGTNIKKANNNSSSEPEGNEVAEAKEPAWTPPQDLLDFIAKARKDRKKI
VYVGFGSIVVADPAKMTQEVIDAVHKADVRCILSKGWSDRLVAKKDDKTEAAAEAEAEAA
TGLVLHADPPEDVLPAEIFPIKSAPHDWLFAQIDAAAHHGGSGTTGASLRAGIPTIIRPF
FGDQFFFGMRVEDLGVGLCLKKWGAMSFARALWEATNSERMIRKAAVLGEQIREENGVDT
AIQSIYRDMEYAKSLVREKAVKSGMLRSGGGTERADRGHGKNDNNTDSDEEAYNDDDDDD
DEPEESWTFVGDDDPMQQSVILTPDAGRASGDDGVVQPASSGAGGSSGSGGYKALGSRIL
GIATAQKQ

Enzyme Prediction     

EC	2.4.1.173:13

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT1	1229	1685	2.2e-55	0.9738219895287958

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340817	GT1_Gtf-like	9.06e-75	1227	1685	1	401	UDP-glycosyltransferases and similar proteins. This family includes the Gtfs, a group of homologous glycosyltransferases involved in the final stages of the biosynthesis of antibiotics vancomycin and related chloroeremomycin. Gtfs transfer sugar moieties from an activated NDP-sugar donor to the oxidatively cross-linked heptapeptide core of vancomycin group antibiotics. The core structure is important for the bioactivity of the antibiotics.
275402	PH-GRAM1_AGT26	1.06e-56	478	592	1	116	Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, repeat 1. ATG26 (also called UGT51/UDP-glycosyltransferase 51), a member of the glycosyltransferase 28 family, resulting in the biosynthesis of sterol glucoside. ATG26 in decane metabolism and autophagy. There are 32 known autophagy-related (ATG) proteins, 17 are components of the core autophagic machinery essential for all autophagy-related pathways and 15 are the additional components required only for certain pathways or species. The core autophagic machinery includes 1) the ATG9 cycling system (ATG1, ATG2, ATG9, ATG13, ATG18, and ATG27), 2) the phosphatidylinositol 3-kinase complex (ATG6/VPS30, ATG14, VPS15, and ATG34), and 3) the ubiquitin-like protein system (ATG3, ATG4, ATG5, ATG7, ATG8, ATG10, ATG12, and ATG16). Less is known about how the core machinery is adapted or modulated with additional components to accommodate the nonselective sequestration of bulk cytosol (autophagosome formation) or selective sequestration of specific cargos (Cvt vesicle, pexophagosome, or bacteria-containing autophagosome formation). The pexophagosome-specific additions include the ATG30-ATG11-ATG17 receptor-adaptors complex, the coiled-coil protein ATG25, and the sterol glucosyltransferase ATG26. ATG26 is necessary for the degradation of medium peroxisomes. It contains 2 GRAM domains and a single PH domain. PH domains are only found in eukaryotes. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. PH domains also have diverse functions. They are often involved in targeting proteins to the plasma membrane, but few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.
275403	PH-GRAM2_AGT26	1.30e-47	1024	1116	1	93	Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, repeat 2. ATG26 (also called UGT51/UDP-glycosyltransferase 51), a member of the glycosyltransferase 28 family, resulting in the biosynthesis of sterol glucoside. ATG26 in decane metabolism and autophagy. There are 32 known autophagy-related (ATG) proteins, 17 are components of the core autophagic machinery essential for all autophagy-related pathways and 15 are the additional components required only for certain pathways or species. The core autophagic machinery includes 1) the ATG9 cycling system (ATG1, ATG2, ATG9, ATG13, ATG18, and ATG27), 2) the phosphatidylinositol 3-kinase complex (ATG6/VPS30, ATG14, VPS15, and ATG34), and 3) the ubiquitin-like protein system (ATG3, ATG4, ATG5, ATG7, ATG8, ATG10, ATG12, and ATG16). Less is known about how the core machinery is adapted or modulated with additional components to accommodate the nonselective sequestration of bulk cytosol (autophagosome formation) or selective sequestration of specific cargos (Cvt vesicle, pexophagosome, or bacteria-containing autophagosome formation). The pexophagosome-specific additions include the ATG30-ATG11-ATG17 receptor-adaptors complex, the coiled-coil protein ATG25, and the sterol glucosyltransferase ATG26. ATG26 is necessary for the degradation of medium peroxisomes. It contains 2 GRAM domains and a single PH domain. PH domains are only found in eukaryotes. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. PH domains also have diverse functions. They are often involved in targeting proteins to the plasma membrane, but few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.
224732	YjiC	2.59e-43	1228	1685	3	395	UDP:flavonoid glycosyltransferase YjiC, YdhE family [Carbohydrate transport and metabolism].
397255	Glyco_transf_28	2.04e-30	1229	1364	1	139	Glycosyltransferase family 28 N-terminal domain. The glycosyltransferase family 28 includes monogalactosyldiacylglycerol synthase (EC 2.4.1.46) and UDP-N-acetylglucosamine transferase (EC 2.4.1.-). This N-terminal domain contains the acceptor binding site and likely membrane association site. This family also contains a large number of proteins that probably have quite distinct activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBZ60676.1|GT1	0.0	121	1703	61	1477
UQC81542.1|GT1	0.0	111	1702	240	1639
AEO60294.1|GT1	0.0	133	1703	59	1438
UNI15418.1|GT1	0.0	125	1706	26	1388
BAH60889.1|GT1	0.0	124	1703	24	1412

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XVM_A	8.04e-165	1209	1695	2	445	Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) [Saccharomyces cerevisiae S288C],5XVM_B Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) [Saccharomyces cerevisiae S288C]
5GL5_A	1.60e-164	1209	1695	22	465	Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c): UDPG complex [Saccharomyces cerevisiae S288C],5GL5_B Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c): UDPG complex [Saccharomyces cerevisiae S288C]
1IIR_A	3.75e-20	1227	1669	1	380	Crystal Structure of UDP-glucosyltransferase GtfB [Amycolatopsis orientalis]
1RRV_A	7.87e-11	1227	1638	1	348	X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin. [Amycolatopsis orientalis],1RRV_B X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin. [Amycolatopsis orientalis]
6KQW_A	1.68e-06	1225	1685	2	381	Chain A, Uncharacterized UDP-glucosyltransferase YjiC [Bacillus subtilis subsp. subtilis str. 168]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WID6|ATG26_ASPFU	0.0	125	1759	6	1373	Sterol 3-beta-glucosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=atg26 PE=3 SV=2
sp|Q0CKU4|ATG26_ASPTN	0.0	391	1703	184	1329	Sterol 3-beta-glucosyltransferase OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=atg26 PE=3 SV=1
sp|Q0UY53|ATG26_PHANO	0.0	405	1698	192	1379	Sterol 3-beta-glucosyltransferase OS=Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) OX=321614 GN=ATG26 PE=3 SV=1
sp|Q2U0C3|ATG26_ASPOR	0.0	125	1703	9	1323	Sterol 3-beta-glucosyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=atg26 PE=3 SV=2
sp|Q7S1I0|ATG26_NEUCR	0.0	133	1702	71	1446	Sterol 3-beta-glucosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=apg-12 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999988	0.000092

TMHMM  Annotations     

There is no transmembrane helices in SPBR_04032-t41_1-p1.