CAZyme Information: SPAC7D4.06c.1-p1

Basic Information

• Species: Schizosaccharomyces pombe
• Lineage: Ascomycota; Schizosaccharomycetes; ; Schizosaccharomycetaceae; Schizosaccharomyces; Schizosaccharomyces pombe
• CAZyme ID: SPAC7D4.06c.1-p1
• CAZy Family: GH76
• CAZyme Description: dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase Alg3

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
406	Spom972h_chrI|CGC27	46991.81	9.7997

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Spombe972h	6995	284812	1857	5138

• Gene Location: location=Spom972h_chrI:2626583-2627803(+)

Full Sequence      

MSSVETRNSFNPFRVLFDLGSYGWLHPSRLLLLEIPFVFAIISKVPYTEIDWIAYMEQVN
SFLLGERDYKSLVGCTGPLVYPGGHVFLYTLLYYLTDGGTNIVRAQYIFAFVYWITTAIV
GYLFKIVRAPFYIYVLLILSKRLHSIFILRLFNDGFNSLFSSLFILSSCKKKWVRASILL
SVACSVKMSSLLYVPAYLVLLLQILGPKKTWMHIFVIIIVQILFSIPFLAYFWSYWTQAF
DFGRAFDYKWTVNWRFIPRSIFESTSFSTSILFLHVALLVAFTCKHWNKLSRATPFAMVN
SMLTLKPLPKLQLATPNFIFTALATSNLIGILCARSLHYQFYAWFAWYSPYLCYQASFPA
PIVIGLWMLQEYAWNVFPSTKLSSLIAVCVPLITILKLYTSDYRKP

Enzyme Prediction     

EC	2.4.1.258:10

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	30	384	6.5e-130	0.989010989010989

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	4.74e-170	30	384	3	358	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
NP_593853.1|GT58|2.4.1.258	2.50e-296	1	406	1	406
CAB16723.2|GT58|2.4.1.258	2.50e-296	1	406	1	406
CAE7199648.1|GT58	3.61e-98	2	395	6	398
QDS73095.1|GT58	1.95e-96	6	388	12	400
QRC96987.1|GT58	4.70e-94	24	395	19	393

PDB Hits     

SPAC7D4.06c.1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9Y7I4|ALG3_SCHPO	4.44e-297	1	406	1	406	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=alg3 PE=3 SV=1
sp|Q9C1K8|ALG3_NEUCR	1.68e-88	25	395	35	415	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=alg-3 PE=3 SV=1
sp|A1CBE6|ALG3_ASPCL	1.31e-82	41	388	97	443	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=alg3 PE=3 SV=1
sp|Q2U6A4|ALG3_ASPOR	9.66e-80	24	388	19	381	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alg3 PE=3 SV=1
sp|Q4WVG2|ALG3_ASPFU	3.56e-78	41	388	43	389	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=alg3 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999737	0.000272

TMHMM  Annotations     

Start	End
73	95
105	124
177	199
214	236
260	282
311	333
345	367
382	399