CAZyme Information: SPAC4H3.03c.1-p1

Basic Information

• Species: Schizosaccharomyces pombe
• Lineage: Ascomycota; Schizosaccharomycetes; ; Schizosaccharomycetaceae; Schizosaccharomyces; Schizosaccharomyces pombe
• CAZyme ID: SPAC4H3.03c.1-p1
• CAZy Family: GH5
• CAZyme Description: glucan 1,4-alpha-glucosidase (predicted)

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
649	Spom972h_chrI|CGC29	74488.97	5.0277

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Spombe972h	6995	284812	1857	5138

• Gene Location: location=Spom972h_chrI:3826640-3828589(-)

Full Sequence      

MPPNARVSYGAEKYLPREIDNHGIVGNMHTSAMISLDGSVEMMCWPNFDSPSIFARILDA
RAGHFSITPIEQTSCKQMYEPSTNILHTKFYSERGVLRLLDFFHRPWEDYEPLYPWLIRR
VSCIRGTSRIKLECFPALDYARQSHETRVSKITENYYQAEFVPASGDPKYILDCVPSGDQ
LKIDLELIYPAEHLIEGGGVISYLELEEGQEITFIFRQEGLGPNVDYVTPNLVDKLEDST
KRYWRAWIQQCVYTGRYREFVQRNALTLKLLIYEPTGAVIASPTFSLPEDLGGVRNWDYR
FTWIRDSAFTIYALAQLGFRAEAVEYMSFIYHVLKKKNKDGGINIVYSIHGDSQNLEEVE
LTHLRGYYNSHPVRIGNAAVHHLQLDIYGELMDSIYIYDKKCQPIAYDLWLEVRQICDYV
CANWKRPDKSIWEVRGQERNFLYSKIMLWVALDRALRIAFTRSLPCPKHMVWMDTRDEIY
EAIMDSGYNMEKGFFAQSFENNDILDASVLVMPLVSFISPTDPRFLSTMDNIMKPLEKDG
LMSNGLIFRYNNFVYEDGVGGDEGAFTMVCFWLVEALAMAGCSGYPKLLSTAVSMFEDLV
RYSSHLQYYSEECSLSSESLGNSPQAFSSIAAIAAAHILDKALSIFNEI

Enzyme Prediction     

No EC number prediction in SPAC4H3.03c.1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH15	258	636	5.1e-75	0.9916897506925207

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225922	SGA1	1.66e-161	8	609	5	573	Glucoamylase (glucan-1,4-alpha-glucosidase), GH15 family [Carbohydrate transport and metabolism].
395586	Glyco_hydro_15	1.49e-114	255	608	1	355	Glycosyl hydrolases family 15. In higher organisms this family is represented by phosphorylase kinase subunits.
273702	oligosac_amyl	4.72e-10	234	576	223	542	oligosaccharide amylase. The name of this type of amylase is based on the characterization of an glucoamylase family enzyme from Thermoactinomyces vulgaris. The T. vulgaris enzyme was expressed in E. coli and, like other glucoamylases, it releases beta-D-glucose from starch. However, unlike previously characterized glucoamylases, this T. vulgaris amylase hydrolyzes maltooligosaccharides (maltotetraose, maltose) more efficiently than starch (1), indicating this enzyme belongs to a class of glucoamylase-type enzymes with oligosaccharide-metabolizing activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
NP_594339.1|GH15	0.0	1	649	1	649
CAA93342.1|GH15	0.0	1	649	1	649
EGX46761.1|GH15	3.36e-227	2	641	9	669
UNI20969.1|GH15	9.46e-211	14	642	26	678
CAP69519.1|GH15	4.89e-210	14	641	46	698

PDB Hits     

SPAC4H3.03c.1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q10211|YAY3_SCHPO	0.0	1	649	1	649	Uncharacterized protein C4H3.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4H3.03c PE=4 SV=1
sp|Q978S7|TREH_THEVO	1.01e-104	18	643	29	614	Trehalase OS=Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) OX=273116 GN=TVG1381191 PE=1 SV=1
sp|Q9HLE2|TREH_THEAC	8.08e-102	33	642	2	571	Trehalase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=Ta0286 PE=1 SV=1
sp|A0R0W9|TREH_MYCS2	5.70e-101	19	639	42	649	Trehalase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_4535 PE=1 SV=1
sp|P71741|TREH_MYCTU	2.13e-100	19	639	54	661	Trehalase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2402 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000053	0.000004

TMHMM  Annotations     

There is no transmembrane helices in SPAC4H3.03c.1-p1.