CAZyme Information: SOCG_04435-t26_1-p1

Basic Information

• Species: Schizosaccharomyces octosporus
• Lineage: Ascomycota; Schizosaccharomycetes; ; Schizosaccharomycetaceae; Schizosaccharomyces; Schizosaccharomyces octosporus
• CAZyme ID: SOCG_04435-t26_1-p1
• CAZy Family: GT4
• CAZyme Description: UDP-glucose-glycoprotein glucosyltransferase Gpt1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1491		171002.75	5.3847

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SoctosporusyFS286	5355	483514	361	4994

• Gene Location: location=KE503206:4114558-4119033(-)

Full Sequence      

MMISNKSRRYIISTTFIQNTLILSHYLKSIFLDTLKNLFGKMRWNKWLTFVPFLSLCFAA
KPIDVKVSTAFSKPSFVALLAESLHEENRDSFLWFLDNLPSIRKDDLITDEQLYNLTVES
LKADKILSQEELTTFSYSVALLSGAPKLAAFSSIVSSHAEDCNTFLVLNEQTNLCFSDLP
EESLIFSENYQPSPLDYKVIGDNLSEGLPVAVIVSDFGVDLDKFHKLYSSLAKNKKCNYI
LRYAPSQDTELEKLYVSSFGAHASLKRTDYLVMDDREIPMSSNENNTSRIDKSKKKKEVL
FDLDSDELETVTQNEIASLDLLASEAIINSRNPLMTFKLLSQDFPMYAQHLVSKVSISDD
LIEDVNHLQSQAIPEGKNAIWLNGISIDLEETDAFSILSWLRKERRILSGFQKLGIEPSK
ASKVLSSEQFDVTEAQFKFFRFHSQDDLEDNKAIFWMNEVETDTRYSKWPKSNMQLLTPI
YPGQLHLLRKQLHTVIYPIFPSSDSSISLLSELVQFSRRPSPVQTGLVCVAPEEDEFGKT
ICRAFHYVARTNSKPSALKFLFKCLAGDPSAGAWALVEEHMPVEDYDEETFHKIKTSLNS
NIYDTILSKSFQWSSRLGLDPESQEVIVNGRIIAHDENYDSNMYSIFIEDIPEIQLAVAQ
EMVSEEENLLDYLLKDAPHTRNPLIYPSSKSKIKQVDMLNLLDDTGYTHSDLLTIGDKKA
KYSIWLVTDFASEEGLELTTTLAEAVSKFESSNLVLVQSDLENELPALITQLSSSSHHTT
EHLLKVLNKNTKKKQSKLTCKVKSKLAKYSEASKAVVAKINNPSQSVLILNGRLINSISV
DKLGVQDLEMLLSHEKSNYIDKLDNALVNATELKKKGILPFVSSYLKKLEFDYVRGSVPG
HEKAMFPRDNFHSQLPPSSLTIGTDNFDSCTYHFVAILDPLSKASQKWSAILESISHLDG
VGVRIYLNPSKGLSDFPLSRFYRYSIEHEPVFRYDGKFKDSGVEFDKLPAETLLTMDIES
RDTWVVMQEEVDIDLYNIKLEHTSSEVLVEPISAVYELKNILIQGFSQEENVKSPPRGMQ
LALETTKHEHFTDTIILANLGYFQLKANPGIWNLKPLPGRSSQFYTITTLNRLDSGKEKQ
VVLDSFEGATLYPVMRRNPGFESSDILDEDMSPQRFFDKMKKKLNFFKNKNKDPTINIFS
VASGHLYERFMYIMTKSVMQHTNKKVKFWFIENFLSPSFKHSIPVLANEYGFEYEYVTYN
WPEWLRKQDEKQREIWGYKILFLDVLFPLDLHKVIFVDADQIVRADLQELMDMDLQGAPY
GYTPMCDSREEMEGFRFWKQGYWKKFLRGLKYHISALYVVDLDRFRKMSAGDLLRRQYQL
LSADPNSLSNLDQDLPNNLQHLIPIHSLPQEWLWCETWCDDQSLKKAKTIDLCQNPLTKE
KKLDRARRQVSEWTTYDNEISAMLRNAEESRLAFTSPVQDLPDNNKETDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1196	1443	4e-131	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1196	1463	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	1.57e-175	1196	1443	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
132996	Glyco_transf_8	3.29e-57	1196	1436	1	241	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
408201	Thioredoxin_14	8.59e-49	442	685	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	1.31e-42	1064	1168	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
NP_595281.1|GT24|2.4.1.-	0.0	42	1491	1	1448
CAC38351.1|GT24|2.4.1.-	0.0	42	1491	1	1448
AAB05993.1|GT24|2.4.1.-	0.0	42	1491	1	1447
CAK39488.1|GT24	4.49e-306	59	1463	28	1455
CAS97678.1|GT24	4.49e-306	59	1463	28	1455

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6TRT_A	3.55e-253	59	1471	6	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5MU1_A	4.98e-253	59	1471	6	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	1.93e-252	59	1471	6	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	1.91e-216	63	1405	4	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	6.13e-210	59	1471	6	1221	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	0.0	42	1491	1	1448	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	3.37e-215	60	1463	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q6P5E4|UGGG1_MOUSE	9.75e-211	60	1463	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	1.51e-207	60	1463	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	2.75e-207	62	1469	29	1513	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.975911	0.024084

TMHMM  Annotations     

There is no transmembrane helices in SOCG_04435-t26_1-p1.