CAZyme Information: SJAG_04363-t26_1-p1

Basic Information

• Species: Schizosaccharomyces japonicus
• Lineage: Ascomycota; Schizosaccharomycetes; ; Schizosaccharomycetaceae; Schizosaccharomyces; Schizosaccharomyces japonicus
• CAZyme ID: SJAG_04363-t26_1-p1
• CAZy Family: GT34
• CAZyme Description: glucosidase I Gls1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
825	KE651167|CGC7	94230.69	4.3271

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SjaponicusyFS275	5232	402676	346	4886

• Gene Location: location=KE651167:2544225-2546819(-)

Full Sequence      

MSSSRSWSRSLLLTCISLCLLSIARCESLLDDVVKRSNDSLLWGAYRPNLYVGVRPRIPK
SLMAGLMWTNVDTYGSISRIRHACEQSEDMGYYGWDSYDARLGGHQVIDDYGMGIKLDVD
FVKLPEAENGQNWALRVRGTPKKGAPEDIKTAMFFYTYLEGEGILEIQKNEENEGSRPYI
VGETPDLGTFLIATKNRFGEHPTFLTAPNNFEDAEETVMDSDYYISLNVEEDDIWKMKDV
LLQLLDQKIRIDLQAKGITSLEDLPPAFEELVLPNVAGNTNVQIVEKVFEGAFEFDIIYN
SVPFLNVVTEDVITAQIESNKAAFKERFEQTFPIAAPFKNESSLQLFSQRAFANLYGGIG
YFYGNSIVSTHGVEDAEDGDYEFAHGFENADMKLQEGTAKLDVERTLFTATPSRPHFARG
FYWDEGFHLIPLGLWDTDLSLEILQSWFSLIDEKGWLGREQILGPEARSQVPAEFQTQYP
DIANPPTLILALKAFIEQMKSYSGAGDFAERALNQEGDGVSGVTSEADLLRVSHLEFPEL
TTKFLRSLYPKLRKHYYWFRDTQAGDLESWERDAFSDVEGYRWRGRTYEHCLASGLDDYP
RAQPPSFAELHVDLMSWMISFTKNLRFVAEFLGEEEDAATFAEYENNMMHNLDDLHWDED
TGMYCDASVDEYDDPLFVCHTGYVSLMPLMLGLLPSDSPKLGRLLEVLRDEDELWSPYGI
RSLSRSDRFYGQGENYWRGPIWINMNYMVLSSLYKNYMQVSGPYQELATAIYNELRVNVV
NTVYREWARSGIFYEQYNSETGVGQRTKGFTGWTSLVVNILSEQY

Enzyme Prediction     

EC	3.2.1.106:25	3.2.1.-:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH63	607	822	4.7e-32	0.4368421052631579

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397353	Glyco_hydro_63	0.0	309	823	2	494	Glycosyl hydrolase family 63 C-terminal domain. This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyze the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the first enzyme in the N-linked oligosaccharide processing pathway. This family represents the C-terminal catalytic domain.
407154	Glyco_hydro_63N	1.51e-76	41	250	1	208	Glycosyl hydrolase family 63 N-terminal domain. This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyze the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the first enzyme in the N-linked oligosaccharide processing pathway. This family represents the N-terminal beta sandwich domain.
225942	GDB1	3.66e-07	600	772	411	566	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
236653	PRK10137	5.03e-07	612	815	578	773	alpha-glucosidase; Provisional
215307	PLN02567	0.003	612	825	328	544	alpha,alpha-trehalase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAB11295.1|GH63	0.0	31	825	32	808
NP_594106.1|GH63	0.0	31	825	32	808
ATZ58403.1|GH63	3.93e-256	32	821	39	807
CCD48755.1|GH63	3.93e-256	32	821	39	807
QSZ29262.1|GH63	4.22e-255	29	821	31	802

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4J5T_A	1.84e-168	30	823	4	802	Crystal structure of Processing alpha-Glucosidase I [Saccharomyces cerevisiae S288C]
5MHF_A	3.97e-120	41	825	37	779	Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus],5MHF_B Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus],5MHF_C Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus],5MHF_D Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O14255|GCS1_SCHPO	0.0	31	825	32	808	Probable mannosyl-oligosaccharide glucosidase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC6G10.09 PE=3 SV=1
sp|P53008|CWH41_YEAST	2.53e-167	31	823	35	832	Mannosyl-oligosaccharide glucosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CWH41 PE=1 SV=1
sp|Q80UM7|MOGS_MOUSE	8.93e-122	41	825	92	834	Mannosyl-oligosaccharide glucosidase OS=Mus musculus OX=10090 GN=Mogs PE=1 SV=1
sp|Q13724|MOGS_HUMAN	1.02e-120	41	825	94	837	Mannosyl-oligosaccharide glucosidase OS=Homo sapiens OX=9606 GN=MOGS PE=1 SV=5
sp|O88941|MOGS_RAT	5.64e-118	43	825	94	834	Mannosyl-oligosaccharide glucosidase OS=Rattus norvegicus OX=10116 GN=Mogs PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000233	0.999727	CS pos: 26-27. Pr: 0.9782

TMHMM  Annotations     

There is no transmembrane helices in SJAG_04363-t26_1-p1.