CAZyme Information: SJAG_02102-t26_1-p1

Basic Information

• Species: Schizosaccharomyces japonicus
• Lineage: Ascomycota; Schizosaccharomycetes; ; Schizosaccharomycetaceae; Schizosaccharomyces; Schizosaccharomyces japonicus
• CAZyme ID: SJAG_02102-t26_1-p1
• CAZy Family: GH5
• CAZyme Description: mannosyltransferase complex subunit Alg1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
428	KE651168|CGC8	48279.65	9.5902

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SjaponicusyFS275	5232	402676	346	4886

• Gene Location: location=KE651168:3018118-3019672(-)

Full Sequence      

MHILIGIAIAVAVLCLYVQRRRCRSGKQVAILVLGDIGHSPRMQYHANSFAKHDWNVELI
GYGDDNNEQELFKKDKRIRCIHIPKTPAWLTPSSKLQFLLFAPLKVTFLWLGLCSILFRV
HAPSYLLVQNPPCIPTFVFALLMRFCFGSRIVIDWHNFGFSILALKLGKNHMLVKIMKAY
ELFLGRFAYKHLCVSNAMSEVLGNWGLKPTYVLYDRPPSHFKPLSKKPYNLLGTAFNPKT
CKLLVSSTSWTPDEDIFVLYKALEEYDAQPNASPILAVITGKGPMKQDFLDHVKEHPLQH
VRFLTPWLSTGDYPRLLACADLGVSLHTSSSGVDLPMKVVDLFGCGIPVLSLPFPAITEL
VKDGRNGKIVGDAHEMAVTIQNLFTNTKELSSLKRGAMSESKHRWDEEWDTVASQVFLMN
NKTAAKKK

Enzyme Prediction     

EC	2.4.1.142:10	2.4.1.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT33	27	415	9e-140	0.9929411764705882

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340843	GT33_ALG1-like	0.0	27	417	4	411	chitobiosyldiphosphodolichol beta-mannosyltransferase and similar proteins. This family is most closely related to the GT33 family of glycosyltransferases. The yeast gene ALG1 has been shown to function as a mannosyltransferase that catalyzes the formation of dolichol pyrophosphate (Dol-PP)-GlcNAc2Man from GDP-Man and Dol-PP-Glc-NAc2, and participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. In humans ALG1 has been associated with the congenital disorders of glycosylation (CDG) designated as subtype CDG-Ik.
215155	PLN02275	1.48e-123	27	384	5	371	transferase, transferring glycosyl groups
223515	RfaB	2.41e-17	103	416	64	370	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
340825	GT4_WbuB-like	6.17e-15	35	406	12	384	Escherichia coli WbuB and similar proteins. This family is most closely related to the GT1 family of glycosyltransferases. WbuB in E. coli is involved in the biosynthesis of the O26 O-antigen. It has been proposed to function as an N-acetyl-L-fucosamine (L-FucNAc) transferase.
340831	GT4_PimA-like	1.28e-12	134	416	91	361	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAB16885.2|GT33	9.13e-152	27	421	25	424
NP_593186.1|GT33	9.13e-152	27	421	25	424
EGX51465.1|GT33	3.65e-111	4	417	13	457
QGA12416.1|GT33	2.83e-110	19	416	33	457
BAE65834.1|GT33	5.10e-107	29	416	45	455

PDB Hits     

SJAG_02102-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O13933|ALG1_SCHPO	1.62e-152	27	421	25	424	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=alg1 PE=3 SV=2
sp|Q59Q79|ALG1_CANAL	1.19e-101	1	417	21	452	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ALG1 PE=3 SV=1
sp|Q6C3K2|ALG1_YARLI	2.62e-99	24	417	37	438	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=ALG1 PE=3 SV=1
sp|Q6BS98|ALG1_DEBHA	1.32e-92	25	417	51	467	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=ALG1 PE=3 SV=2
sp|Q921Q3|ALG1_MOUSE	1.09e-90	3	409	9	453	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Mus musculus OX=10090 GN=Alg1 PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000026	0.000016

TMHMM  Annotations     

Start	End
4	18
98	120
125	147