CAZyme Information: SJAG_02076-t26_1-p1

Basic Information

• Species: Schizosaccharomyces japonicus
• Lineage: Ascomycota; Schizosaccharomycetes; ; Schizosaccharomycetaceae; Schizosaccharomyces; Schizosaccharomyces japonicus
• CAZyme ID: SJAG_02076-t26_1-p1
• CAZy Family: GH47
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1107		130415.85	6.8830

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SjaponicusyFS275	5232	402676	346	4886

• Gene Location: location=KE651168:2959199-2962522(+)

Full Sequence      

MKTLAWIGIISWLTGISQATIVKHDDWSLDNWAEAQKGVAFGHLLDNIGDSGKYAKDVDP
GCIIASPSKENPNYFYQWVRDSAIVIMNLCDKFLEGDHKLEPIIHKYMDESIRLQKTPNP
SGDYYNGGLGEPKFNTDGSAFTEQWGRPQNDSPALRAMAFMKYLNYLLENGASPSEFPHW
IEAVLADLDYTVAHWMNESFDLWEEVQDIHYFTLTVQKAALENGAHFAKRIGAVDQGVLY
ERVVYSIQLKLGEFWNPKKGWIDAYKNRPERMGLDCSTLLASIYSNQFDMQTLPTLLSMQ
QVMSRIYPINAGYKFAMGRYPEDIYNGIDKTIGNPWFICTSTAAHVIYKAVDYYDTYGLP
ELTGANIHFFMHFSEYGDPYNWPVVRKNLLAYADRFLEVVAEFQLPNGSMSEQFSREDGH
QMGARDLSWSYSSLLSTLRAREKVVKKHYPEHKGRKCKLVCEPEGMWDHEHKENWEPEHK
EHCKPEDHKHKEPCKPEHHEHHHEHKEPCKPEHKPEKPCKPEEHCKPEPKKPCKPEEHCK
PVEPCKPEPKKPCKPEEHCKPEPKKPCKPEEPKPKPEKPCKPEDHDHKHKRPCKPGEDEH
KDNCEHKRPCKPGEDEHKDNCQHKHDHKDEHKRPCKPGEDEHKDNCEHKRPCKPGEDEHK
NNCQHKPDHKDEHKRPCKPGEDEHKDNCEHKRPCKPGEDEHKNNCQHKPDHKDEHKRPCK
PGEDEHKDNCEHKRPCKPGEDEHKNNCQHKPDHKDEHKRPCKPGEDEHKNNCQHKPDHKD
EHKRPCKPGEDEHKDNCEHKRPCKPGEDEHKNNCQYKHDHDHEPKRPCKPGEDEHKNNCE
FKRPCKPGEDERKNNCQYKHDHDHEPKRPCKPGEDEHKNNCEFKRPCKPGEDERKNNCQH
KPDHKDEHKDEHKPDHKDGHKDEHKPDHKDGHKDEHKRPCKPGEDEHKDNCEFKRPCKPG
EDERKNNCEFKRPCKPGEDERKNNCEFKRPCKPGEDERKNNCVFKHDHKDEHKHDHKDEH
KDEHKDEHKDEHKRPCKPGEDEHKDNCEFKHDHEHKHDHKDEHKRPCKPGEDEHKDNCKY
KHDHKDEDGHDRMKPKNNDHHEKPEHN

Enzyme Prediction     

EC	3.2.1.3:94

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH15	45	436	2.4e-75	0.9778393351800554

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395586	Glyco_hydro_15	6.11e-101	38	426	1	404	Glycosyl hydrolases family 15. In higher organisms this family is represented by phosphorylase kinase subunits.
225922	SGA1	4.67e-45	25	426	150	589	Glucoamylase (glucan-1,4-alpha-glucosidase), GH15 family [Carbohydrate transport and metabolism].
273344	2A1904	7.43e-09	728	1035	636	893	K+-dependent Na+/Ca+ exchanger. [Transport and binding proteins, Cations and iron carrying compounds]
273702	oligosac_amyl	1.54e-08	57	340	270	536	oligosaccharide amylase. The name of this type of amylase is based on the characterization of an glucoamylase family enzyme from Thermoactinomyces vulgaris. The T. vulgaris enzyme was expressed in E. coli and, like other glucoamylases, it releases beta-D-glucose from starch. However, unlike previously characterized glucoamylases, this T. vulgaris amylase hydrolyzes maltooligosaccharides (maltotetraose, maltose) more efficiently than starch (1), indicating this enzyme belongs to a class of glucoamylase-type enzymes with oligosaccharide-metabolizing activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
NP_595908.1|GH15	1.31e-212	28	444	30	446
CAY05398.1|GH15	1.31e-212	28	444	30	446
CAA18423.1|GH15	1.31e-212	28	444	30	446
BAB60870.1|GH15	3.23e-110	208	444	1	237
EGX49391.1|CBM21|GH15	3.44e-82	29	447	137	565

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1AGM_A	7.20e-78	28	451	2	438	Refined structure for the complex of acarbose with glucoamylase from Aspergillus awamori var. x100 to 2.4 angstroms resolution [Aspergillus awamori],1DOG_A REFINED STRUCTURE FOR THE COMPLEX OF 1-DEOXYNOJIRIMYCIN WITH GLUCOAMYLASE FROM (ASPERGILLUS AWAMORI) VAR. X100 TO 2.4 ANGSTROMS RESOLUTION [Aspergillus awamori],1GLM_A REFINED CRYSTAL STRUCTURES OF GLUCOAMYLASE FROM ASPERGILLUS AWAMORI VAR. X100 [Aspergillus awamori],3GLY_A REFINED CRYSTAL STRUCTURES OF GLUCOAMYLASE FROM ASPERGILLUS AWAMORI VAR. X100 [Aspergillus awamori]
1GAH_A	7.40e-78	28	451	2	438	GLUCOAMYLASE-471 COMPLEXED WITH ACARBOSE [Aspergillus awamori]
1GAI_A	7.61e-78	28	451	2	438	GLUCOAMYLASE-471 COMPLEXED WITH D-GLUCO-DIHYDROACARBOSE [Aspergillus awamori]
3EQA_A	1.76e-76	28	451	2	439	Catalytic domain of glucoamylase from aspergillus niger complexed with tris and glycerol [Aspergillus niger]
6FRV_A	6.70e-75	28	451	2	439	Structure of the catalytic domain of Aspergillus niger Glucoamylase [Aspergillus niger]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O60087|AMYG_SCHPO	2.32e-213	28	444	30	446	Probable glucoamylase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=meu17 PE=2 SV=1
sp|P36914|AMYG_ASPOR	1.06e-76	29	445	30	459	Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2
sp|D8Q9M3|AMYG_SCHCM	1.17e-76	30	441	26	451	Glucoamylase ARB_02327-1 OS=Schizophyllum commune (strain H4-8 / FGSC 9210) OX=578458 GN=SCHCODRAFT_57589 PE=1 SV=1
sp|P22832|AMYG_ASPUS	1.19e-74	28	451	26	462	Glucoamylase OS=Aspergillus usamii OX=186680 GN=glaA PE=3 SV=1
sp|P69328|AMYG_ASPNG	5.88e-74	28	451	26	463	Glucoamylase OS=Aspergillus niger OX=5061 GN=GLAA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000249	0.999713	CS pos: 19-20. Pr: 0.9746

TMHMM  Annotations     

There is no transmembrane helices in SJAG_02076-t26_1-p1.