CAZyme Information: SDRG_05323-t26_1-p1

Basic Information

• Species: Saprolegnia diclina
• Lineage: Oomycota; NA; ; Saprolegniaceae; Saprolegnia; Saprolegnia diclina
• CAZyme ID: SDRG_05323-t26_1-p1
• CAZy Family: GH17
• CAZyme Description: dolichyl-diphosphooligosaccharide-protein glycosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
886	JH767145|CGC1	97812.69	7.4645

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SdiclinaVS20	17448	1156394	89	17359

• Gene Location: location=JH767145:146903-149605(-)

Full Sequence      

MAKPVTKAAPSVAAEAKGDDPAFATISGSVMDQSQVIFGVPVSSPPGYDIEDDSSTLAPV
LKYGLLAAICLMSFSIRLFSIVRYESVIHEFDPYFNFRTTKYLASEGFLEFLDWFDDRAW
YPLGRIIGGTIYPGLMYTAALVYWVLNWLNISINVRNTCVFLAPMFAANTAIASYMLTKE
VTKRTSAGLLAAAFAGVVPSYISRSVGGSYDNEGVAIFALIFVFYLWIKAVNTGSMFWAA
ACSLAYFYMVAAWGGYVFIINMIPIYVLVMIFAGRYTPRLYIAYSTFYALGSLMAMQVPF
VGFNVLKQAECAGSHGVFLLLQVYCFVNWLRGFISAGAFRRLVVVGAATLVAGVAVALVL
LQLMGKVQWTGRSLTLLDPTYASKYIPIIASVSEHQPTTWTSYFFDLHILVPFAPVGLYF
MYKNLTDGGIFVILYGTVAWYFAGVMVRLMLTLAPIACILAAVGISATLRKFMGFLHRSF
SGTTTPLKNGVQEVHSGFALIVVMVLTALLLSYQFHAAYVSSMAYSSPSIVIEAGRTQSG
ERVVFDDYREAYFWLRQNTAADARILSWWDYGYQMSGMANRTVIVDNNTWNNTHIATVGR
ALASTEEGAYPILQSLDVDYVLVIFGGLTGYSSDDINKFLWPVRIGSGVFPNDMPAERDF
YSASGNFDVGPGGSKILHNCLAYKLCYYRFGEMRTDYQHPPGFDRARNTEVGVKNIKLTH
MEEAFTSEHWIVRIFKVKKQPNVQPTTEEMKRKLRDAASQTASIDTEKTRFVGCVTGEDM
LGADKIYSGGATGANYNLALHHAKAHGKRYFALSRVGGEGHVFAFDKLALAEKDFDGNGA
GCERPCMDSQAHFCGCADSGCSDALAQPGKGQEHNRRWAIYEREEA

Enzyme Prediction     

EC	2.4.99.18:21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT66	64	694	1.8e-168	0.84992784992785

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396873	STT3	3.67e-106	60	526	1	478	Oligosaccharyl transferase STT3 subunit. This family consists of the oligosaccharyl transferase STT3 subunit and related proteins. The STT3 subunit is part of the oligosaccharyl transferase (OTase) complex of proteins and is required for its activity. In eukaryotes, OTase transfers a lipid-linked core-oligosaccharide to selected asparagine residues in the ER. In the archaea STT3 occurs alone, rather than in an OTase complex, and is required for N-glycosylation of asparagines.
224206	Stt3	6.34e-61	55	739	13	731	Asparagine N-glycosylation enzyme, membrane subunit Stt3 [Posttranslational modification, protein turnover, chaperones].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ23018.1|GT66	0.0	25	883	28	882
CCA15036.1|GT66	0.0	51	881	51	877
CCA23157.1|GT66	0.0	51	881	51	877
AFJ69334.1|GT66	0.0	51	882	53	890
ACI33169.1|GT66	9.39e-275	60	738	17	695

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6FTJ_5	5.42e-275	60	738	17	695	Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Non-programmed 80S Ribosome [Canis lupus familiaris]
6FTG_5	7.46e-275	60	738	17	695	Subtomogram average of OST-containing ribosome-translocon complexes from canine rough microsomal membranes [Canis lupus familiaris],6FTI_5 Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Programmed 80S Ribosome [Canis lupus familiaris]
6S7O_A	2.12e-274	60	738	17	695	Cryo-EM structure of human oligosaccharyltransferase complex OST-A [Homo sapiens]
6S7T_A	7.45e-262	60	742	71	777	Cryo-EM structure of human oligosaccharyltransferase complex OST-B [Homo sapiens]
6C26_A	2.08e-216	55	770	10	706	The Cryo-EM structure of a eukaryotic oligosaccharyl transferase complex [Saccharomyces cerevisiae S288C],6EZN_F Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex [Saccharomyces cerevisiae S288C],7OCI_F Chain F, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 [Saccharomyces cerevisiae S288C]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q54NM9|STT3_DICDI	3.06e-278	60	738	41	708	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 OS=Dictyostelium discoideum OX=44689 GN=stt3 PE=3 SV=1
sp|F1PJP5|STT3A_CANLF	1.91e-274	60	738	17	695	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Canis lupus familiaris OX=9615 GN=STT3A PE=1 SV=3
sp|P46978|STT3A_MOUSE	2.71e-274	60	738	17	695	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Mus musculus OX=10090 GN=Stt3a PE=1 SV=1
sp|P46977|STT3A_HUMAN	1.09e-273	60	738	17	695	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Homo sapiens OX=9606 GN=STT3A PE=1 SV=2
sp|Q5RCE2|STT3A_PONAB	1.09e-273	60	738	17	695	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Pongo abelii OX=9601 GN=STT3A PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.994345	0.005673

TMHMM  Annotations     

Start	End
59	81
124	146
159	176
186	208
215	237
252	274
281	303
313	335
342	364
399	421
428	447
451	473
494	516