CAZyme Information: SDRG_04081-t26_1-p1

Basic Information

• Species: Saprolegnia diclina
• Lineage: Oomycota; NA; ; Saprolegniaceae; Saprolegnia; Saprolegnia diclina
• CAZyme ID: SDRG_04081-t26_1-p1
• CAZy Family: GH16
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
440	JH767141|CGC1	49688.81	6.1308

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SdiclinaVS20	17448	1156394	89	17359

• Gene Location: location=JH767141:242477-244220(-)

Full Sequence      

MEEASKHVVVLVLGDIGRSPRMQYHAISLAEMPDTRVTVVGYEGERCVPQLLAKPNIALR
TFAPIAVSRKLFVLSGPLKVLLQLFQLFWLLLFCVGSFDVLLVQNPPTIPTLYIGWLSCK
LKRAKLVIDWHNMGYTVLALSIGQGHVFVKIARWFERFFGRRADGHFCVTEAMQLWLQDN
WDISPVVLYDKPPAFFQPTPLDVQHDLLVRLDDAFAAVADLVPAKPNATWRTELLGSSSF
KLRNDRPALVISSTSWTEDEDFGLLFDALVLFEEVAKDRCDIPSLVVAVTGKGPQKAMYL
ERIAALKFQRVRIVTLWLEASDYPLLLGAADLGICLHTSTSGLDLPMKVLDMFGCRVPVC
AVGFNCLNELIKHDKNGLVFQRSEELCDQLIELLTAYPKNETLTRYRANLSDFEHWPENW
SKHAAPVFDALMAQHYDETE

Enzyme Prediction     

EC	2.4.1.142:10	2.4.1.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT33	6	426	4.5e-156	0.9929411764705882

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340843	GT33_ALG1-like	1.07e-175	4	428	2	411	chitobiosyldiphosphodolichol beta-mannosyltransferase and similar proteins. This family is most closely related to the GT33 family of glycosyltransferases. The yeast gene ALG1 has been shown to function as a mannosyltransferase that catalyzes the formation of dolichol pyrophosphate (Dol-PP)-GlcNAc2Man from GDP-Man and Dol-PP-Glc-NAc2, and participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. In humans ALG1 has been associated with the congenital disorders of glycosylation (CDG) designated as subtype CDG-Ik.
215155	PLN02275	1.95e-169	6	394	5	371	transferase, transferring glycosyl groups
223515	RfaB	7.46e-12	98	424	84	367	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
340825	GT4_WbuB-like	2.32e-10	98	394	99	360	Escherichia coli WbuB and similar proteins. This family is most closely related to the GT1 family of glycosyltransferases. WbuB in E. coli is involved in the biosynthesis of the O26 O-antigen. It has been proposed to function as an N-acetyl-L-fucosamine (L-FucNAc) transferase.
340831	GT4_PimA-like	7.65e-09	96	395	81	332	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ21318.1|GT33	5.11e-168	3	439	78	570
CCA25895.1|GT33	1.01e-158	7	432	11	451
AAH55554.1|GT33	2.53e-126	2	437	51	488
NP_956161.1|GT33	2.53e-126	2	437	51	488
AFE69158.1|GT33	9.12e-126	4	427	19	448

PDB Hits     

SDRG_04081-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9BT22|ALG1_HUMAN	1.24e-123	4	427	31	460	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Homo sapiens OX=9606 GN=ALG1 PE=1 SV=2
sp|Q5R7A2|ALG1_PONAB	2.47e-123	5	427	32	460	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Pongo abelii OX=9601 GN=ALG1 PE=2 SV=1
sp|Q8L7M0|TUN_ARATH	6.59e-121	10	434	9	461	UDP-glycosyltransferase TURAN OS=Arabidopsis thaliana OX=3702 GN=TUN PE=2 SV=1
sp|Q921Q3|ALG1_MOUSE	5.21e-116	5	427	32	460	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Mus musculus OX=10090 GN=Alg1 PE=1 SV=3
sp|P90522|ALG1_DICDI	2.56e-95	8	420	4	455	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Dictyostelium discoideum OX=44689 GN=alg1 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000054	0.000000

TMHMM  Annotations     

There is no transmembrane helices in SDRG_04081-t26_1-p1.