CAZyme Information: SDRG_01273-t26_1-p1

Basic Information

• Species: Saprolegnia diclina
• Lineage: Oomycota; NA; ; Saprolegniaceae; Saprolegnia; Saprolegnia diclina
• CAZyme ID: SDRG_01273-t26_1-p1
• CAZy Family: AA3
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1071		117246.45	5.1146

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SdiclinaVS20	17448	1156394	89	17359

• Gene Location: location=JH767134:442399-445654(-)

Full Sequence      

MKRRAALLVLLVVALSTKALTHVPKPSATTAPPLARPSADGDHRTSLDGVCRTFHMSGSW
WRYEWCHQEHVRQYAIDRVTGAEASVNLIGRFFGATDGEASPTRARPFVTHVFLNGDACH
GGSRQSPVARNRSAEVRFSCCSFRPTETYIESVSEPILCEYLLSVCTPLACATKLQAQDE
SSDDTKRLRATVKTMFYHAYNNYMEHAFPLDNLRPISCKGDTFELGKIPMLTLIDTLDTL
VVFGDFEEFRRAVRLVLSGASFDLNTEVSVFETTIRVLGGLLSAHMFAVDASLGIYEASA
YDDGLLRLAIDLADRLLPAFVTKTGIPYGTVNLRYGVPRGETTVASTAGAGSLTMEFTML
SVLTGNPVYALAARKAVRALFERKSSVGLLGKHIDVDSGDWTETISGPGSNSDSFYEYLL
KMYVLFGDAESLGMFEEVYSNVMEHNKHGDWYGDVSMWDGCTGGAAIVFDNLVAFWPGMQ
TLLGDFETSAKSLNAYYQVWHKYGFLPEQFDVGRWRPKKASSNRYPLRPELIESTFYMHA
ATRDDSWRKAGAAFVASIERFSKTTCGYASIADVESRAQEDDMPSFFLSETCKYLYLLFD
DNNLLRRGNYVFTTEAHPFPVLSSAQVAAIWPPNGAPRAPRQALTCPRLPFYASVSFDAS
YQDAFVKMKPRCDAPRRAAAKASKKNSAKATAPRVLHGGDALGDFVIDQLVGGFRARSAR
FPGDALKVTNLGDPKIVVEFHSPEPTPLADFRIHNFETDVTTRCRLLVYDADDVLIDTIT
CSGALFGPTKTEGSSVFVEKRSVELASPLRACAAMDAVDPGAIVLVQRGDCYFDDKARRG
MDAGASAVIIVNTDAAEDLMIMARSSTSDAAQDETPISVPIVMVAQTAEARLRRPHTRVS
LEHHIVPRDDDDFPYVVGSRYDLKVVGPHGWGLHLALKTSDATGVSAWTIALVDATAPEE
EETASDETTPTAQAFARVLQQLKGLGFSDEALHLLENEDDADLRLEALLSGLRDVGLDDM
ADKVALKTSDAKVAKDDDPPQVIEAFQSLASEEDASDVCTDADAPAKVPGR

Enzyme Prediction     

EC	3.2.1.113:20

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH47	195	621	1.5e-137	0.9977578475336323

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396217	Glyco_hydro_47	3.01e-147	195	621	1	453	Glycosyl hydrolase family 47. Members of this family are alpha-mannosidases that catalyze the hydrolysis of the terminal 1,2-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).
240427	PTZ00470	1.97e-84	174	621	60	518	glycoside hydrolase family 47 protein; Provisional
239038	PA_C_RZF_like	9.22e-14	771	888	16	129	PA_C-RZF_ like: Protease-associated (PA) domain C_RZF-like. This group includes various PA domain-containing proteins similar to C-RZF (chicken embryo RING zinc finger) protein. These proteins contain a C3H2C3 RING finger. C-RZF is expressed in embryo cells and is restricted mainly to brain and heart, it is localized to both the nucleus and endosomes. Additional C3H2C3 RING finger proteins belonging to this group, include Arabidopsis ReMembR-H2 protein and mouse sperizin. ReMembR-H2 is likely to be an integral membrane protein, and to traffic through the endosomal pathway. Sperizin is expressed in haploid germ cells and localized in the cytoplasm, it may participate in spermatogenesis. The significance of the PA domain to these proteins has not been ascertained. It may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate.
240122	PA_subtilisin_1	1.28e-12	784	893	3	105	PA_subtilisin_1: Protease-associated domain containing subtilisin-like proteases, subgroup 1. A subgroup of PA domain-containing subtilisin-like proteases. The significance of the PA domain to many of the proteins in which it is inserted is undetermined. It may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate. Proteins into which the PA domain is inserted include the following subtilisin-like proteases: i) melon cucumisin, ii) Arabidopsis thaliana Ara12, iii) Alnus glutinosa ag12, iv) members of the tomato P69 family, and v) tomato LeSBT2. However, these proteins belong to other subtilisin-like subgroups. Relatively little is known about proteins in this subgroup.
239047	PA_GO-like	6.67e-12	777	892	17	125	PA_GO-like: Protease-associated domain containing proteins like Arabidopsis thaliana growth-on protein GRO10. This group contains various PA domain-containing proteins similar to the functionally uncharacterized Arabidopsis GRO10. The PA domain may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG56334.1|GH47	0.0	1	1032	1	1029
AIG55397.1|GH47	0.0	48	1035	30	1025
UIZ27914.1|GH47	8.17e-233	132	936	19	1012
QCQ78383.1|GH47	4.80e-134	161	620	16	478
CAY09791.1|GH47	6.02e-134	169	621	21	477

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5KKB_A	5.92e-62	180	622	13	468	Structure of mouse Golgi alpha-1,2-mannosidase IA and Man9GlcNAc2-PA complex [Mus musculus],5KKB_B Structure of mouse Golgi alpha-1,2-mannosidase IA and Man9GlcNAc2-PA complex [Mus musculus]
1NXC_A	7.37e-62	180	622	11	466	Structure of mouse Golgi alpha-1,2-mannosidase IA reveals the molecular basis for substrate specificity among Class I enzymes (family 47 glycosidases) [Mus musculus]
4AYO_A	4.53e-55	177	617	2	431	Structure of The GH47 processing alpha-1,2-mannosidase from Caulobacter strain K31 [Caulobacter sp. K31],4AYP_A Structure of The GH47 processing alpha-1,2-mannosidase from Caulobacter strain K31 in complex with thiomannobioside [Caulobacter sp. K31],4AYQ_A Structure of The GH47 processing alpha-1,2-mannosidase from Caulobacter strain K31 in complex with mannoimidazole [Caulobacter sp. K31],4AYR_A Structure of The GH47 processing alpha-1,2-mannosidase from Caulobacter strain K31 in complex with noeuromycin [Caulobacter sp. K31],5MEH_A Crystal structure of alpha-1,2-mannosidase from Caulobacter K31 strain in complex with 1-deoxymannojirimycin [Caulobacter sp. K31],5NE5_A Crystal structure of family 47 alpha-1,2-mannosidase from Caulobacter K31 strain in complex with kifunensine [Caulobacter sp. K31]
1FO2_A	6.13e-55	195	625	17	460	Crystal Structure Of Human Class I Alpha1,2-Mannosidase In Complex With 1-Deoxymannojirimycin [Homo sapiens],1FO3_A Crystal Structure Of Human Class I Alpha1,2-Mannosidase In Complex With Kifunensine [Homo sapiens]
5KIJ_A	9.41e-55	195	621	12	451	Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase and Man9GlcNAc2-PA complex [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9FG93|MNS4_ARATH	5.36e-133	177	628	29	480	Alpha-mannosidase I MNS4 OS=Arabidopsis thaliana OX=3702 GN=MNS4 PE=1 SV=1
sp|Q9SXC9|MNS5_ARATH	2.69e-117	186	624	35	481	Alpha-mannosidase I MNS5 OS=Arabidopsis thaliana OX=3702 GN=MNS5 PE=1 SV=1
sp|Q9BV94|EDEM2_HUMAN	8.27e-117	179	618	26	479	ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Homo sapiens OX=9606 GN=EDEM2 PE=1 SV=2
sp|Q8BJT9|EDEM2_MOUSE	1.17e-115	165	618	12	479	ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Mus musculus OX=10090 GN=Edem2 PE=1 SV=1
sp|Q925U4|EDEM1_MOUSE	5.97e-109	187	617	124	577	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Mus musculus OX=10090 GN=Edem1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000668	0.999313	CS pos: 19-20. Pr: 0.9824

TMHMM  Annotations     

There is no transmembrane helices in SDRG_01273-t26_1-p1.