CAZyme Information: SDRG_01271-t26_1-p1

Basic Information

• Species: Saprolegnia diclina
• Lineage: Oomycota; NA; ; Saprolegniaceae; Saprolegnia; Saprolegnia diclina
• CAZyme ID: SDRG_01271-t26_1-p1
• CAZy Family: AA3
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
409		44998.13	7.9250

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SdiclinaVS20	17448	1156394	89	17359

• Gene Location: location=JH767134:438277-439596(-)

Full Sequence      

MHRVVRTVLLLLALAIPPASSLHAYRNVVYFLEWGIYRDFHIANLDWSRITHVNYAFAMP
QVDGSITFLDEFAATQRVYGRDLGIGAVQGCFGQANKYKRAFRGTKFGLSFGGWSGSGPF
PTIAASPSLRATFVANAVALLEDLGLDFIDLDWEYPTLAQLPHFATLLADMRAALHALPF
RTELSIAAPGFPANWGKESMAAICSHVDFLNLMTYEYTGAWSRRAGYHSNLFPNPHAPDG
LRSSVSSAVQYYLTRGCPSEKLVMGVPLYGKSFERTRGLFAPFAPPTSGSHAPDGLWDYK
DIARHASPTFDAVAQATYAYNATTHTVLVYDDPKSVAAKVAYMQAHRLGGLMFWEASGDA
AAGSSASLLTTYASLVGKEDMDFRPNNLWYPHSRYDNIRRPPLLGGFTP

Enzyme Prediction     

No EC number prediction in SDRG_01271-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	24	366	9.1e-73	0.9628378378378378

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
214753	Glyco_18	5.15e-102	26	359	1	334	Glyco_18 domain.
119365	GH18_chitinase	3.45e-98	27	359	1	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
395573	Glyco_hydro_18	1.48e-82	26	359	1	307	Glycosyl hydrolases family 18.
225862	ChiA	5.86e-74	23	371	36	431	Chitinase, GH18 family [Carbohydrate transport and metabolism].
119351	GH18_chitolectin_chitotriosidase	1.84e-73	28	354	2	336	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG56507.1|GH18	4.07e-124	14	399	15	421
AIG56062.1|GH18	3.97e-120	12	399	12	421
AIG55712.1|GH18	4.19e-117	11	399	10	419
AIG55585.1|GH18	1.05e-115	11	399	4	394
AIG56034.1|GH18	1.49e-114	26	409	23	426

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EPB_A	2.47e-81	23	399	36	419	Structure of Chitinase 42 from Trichoderma harzianum [Trichoderma harzianum]
6YLJ_A	3.03e-79	23	399	36	419	Chain A, Endochitinase 42 [Trichoderma harzianum],6YN4_A Chain A, Endochitinase 42 [Trichoderma harzianum],7AKQ_A Chain A, Endochitinase 42 [Trichoderma harzianum]
3G6L_A	8.17e-71	27	399	23	402	The crystal structure of a chitinase CrChi1 from the nematophagous fungus Clonostachys rosea [Clonostachys rosea],3G6M_A crystal structure of a chitinase CrChi1 from the nematophagous fungus Clonostachys rosea in complex with a potent inhibitor caffeine [Clonostachys rosea]
6IGY_A	3.91e-69	25	400	16	401	Crystal structure of Aspergillus niger chitinase B [Aspergillus niger]
1D2K_A	2.88e-67	25	399	3	387	Chain A, CHITINASE 1 [Coccidioides immitis],1LL4_A Chain A, Chitinase 1 [Coccidioides immitis],1LL4_B Chain B, Chitinase 1 [Coccidioides immitis],1LL4_C Chain C, Chitinase 1 [Coccidioides immitis],1LL4_D Chain D, Chitinase 1 [Coccidioides immitis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P48827|CHI42_TRIHA	1.27e-80	23	399	36	419	Endochitinase 42 OS=Trichoderma harzianum OX=5544 GN=chit42 PE=1 SV=1
sp|P32470|CHI1_APHAL	1.27e-80	13	400	20	420	Chitinase 1 OS=Aphanocladium album OX=12942 GN=CHI1 PE=1 SV=2
sp|A6N6J0|CHI46_TRIHA	3.40e-79	23	399	37	426	Endochitinase 46 OS=Trichoderma harzianum OX=5544 GN=chit46 PE=1 SV=1
sp|E9ERT9|CHI1_METRA	3.88e-76	25	400	39	421	Endochitinase 1 OS=Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) OX=655844 GN=chit1 PE=3 SV=1
sp|O14456|CHI1_METAN	2.84e-68	25	400	39	420	Endochitinase 1 OS=Metarhizium anisopliae OX=5530 GN=chit1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000236	0.999740	CS pos: 21-22. Pr: 0.9782

TMHMM  Annotations     

There is no transmembrane helices in SDRG_01271-t26_1-p1.