CAZyme Information: SAPIO_CDS8792-t41_1-p1

Basic Information

• Species: Scedosporium apiospermum
• Lineage: Ascomycota; Sordariomycetes; ; Microascaceae; Scedosporium; Scedosporium apiospermum
• CAZyme ID: SAPIO_CDS8792-t41_1-p1
• CAZy Family: GT2
• CAZyme Description: Beta-mannosidase-like protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
873		99311.78	5.4162

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SapiospermumIHEM14462	10920	N/A	2544	8376

• Gene Location: location=JOWA01000132:113879-116638(+)

Full Sequence      

MARKQHYLTAWEWRAGDTNGICQADSLKEWKAVASFPSVVQMELAAHKLIPDYKIGENER
AIQWVGEVDWEYRTSFATPEVQGNIDLVFEGLDTVATVTLNGTEILKSDNMFIPARVPVK
EHLKPAGEANELHIVFESPVKAGKVLEGKYGERKSMMRDRKRMHLRKAQCHWGWDWGPVV
LTSGPYLPVYLDVYETRIDSVQVRTTLKRDHSASLATTIIEAVNAKEGTSAKVTVTEGTS
EIGTGTTTLDASGKGLVSLDIKSPKLWWPSGQGDQNLYTAHVALVSSDQQTVDETSTRFG
IRTVELIQTPLKDEPGTTFMFRVNGRDIFTQGGDWIPADNLLPTIMRERYFDWMRLAKFN
NLNMIRVWGGGIYETDDFFDACDENGLLVWHDYAFACGDWPIHDEYIESVKKEAVVHTKR
MRNHPSLALLCGGNEDFMLNDWDKVEYDHDDLVGPFDNTNFPQRKIFLDVLPKVAKEHCP
DVAYWANSPWGGRTANDTTVGDIHQWSVWHLEQLPYQEYKNVSGRFVSEFGMHGFPIKRT
VDHFTRGARPEERHPQSRLIDCHNKGHGAHTRIARYLAENFRFDMTSLDNFVYSSQLMQS
EAYGYALRSWKRLFNGEGEQKCAGAIIWQFNDIYPVTSWAYVDYFLRPKPAFYTIRREFA
PVTVGVERKPATRWVDEDKANASVVPSFEIFAHNTSSEEVVCDLVLRAYDFASGQYTELG
DATKRTVSLKAGRNTEIRTLKSVPSWTEDTLVVLEATLVSGSGKELARFVDWPEPYRYLY
WPTGTNLSVTVTDAAENSEGWESIVTVSSEQALKGVWLEPVYDGSEKEEDPEPLWEDNMV
DLLPGSERALRVKGLKGRKVHGRFLADWEVKRE

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	5	739	2.3e-90	0.6888297872340425

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	6.17e-69	35	675	29	643	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
395572	Glyco_hydro_2	7.70e-17	196	302	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
236673	ebgA	4.31e-10	72	435	113	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
236657	PRK10150	1.28e-09	63	305	62	279	beta-D-glucuronidase; Provisional
407658	Mannosidase_ig	7.12e-07	688	778	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QYT00148.1|GH2	0.0	8	871	8	881
CCD56258.2|GH2	4.31e-220	25	865	20	853
AEO69937.1|GH2	7.33e-220	11	865	13	848
AFJ59925.1|GH2|3.2.1.25	1.18e-219	11	865	13	852
ATZ49691.1|GH2	2.52e-219	25	865	43	876

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	2.50e-120	28	853	41	811	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	2.37e-110	9	664	13	656	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.48e-110	9	664	15	658	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	2.54e-110	9	664	15	658	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	6.60e-110	9	664	13	656	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|I2C092|MANBB_THETO	2.09e-220	11	865	13	852	Beta-mannosidase B OS=Thermothelomyces thermophilus OX=78579 GN=man9 PE=1 SV=1
sp|A1CGA8|MANBB_ASPCL	8.92e-186	1	856	1	832	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
sp|Q5B7W2|MANBB_EMENI	6.41e-182	30	856	26	830	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
sp|Q4WAH4|MANBB_ASPFU	7.55e-181	1	856	1	832	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
sp|B0YBU9|MANBB_ASPFC	2.98e-180	1	856	1	832	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000039	0.000003

TMHMM  Annotations     

There is no transmembrane helices in SAPIO_CDS8792-t41_1-p1.