CAZyme Information: SAPIO_CDS5959-t41_1-p1

Basic Information

• Species: Scedosporium apiospermum
• Lineage: Ascomycota; Sordariomycetes; ; Microascaceae; Scedosporium; Scedosporium apiospermum
• CAZyme ID: SAPIO_CDS5959-t41_1-p1
• CAZy Family: GH3
• CAZyme Description: putative endo-beta-1,4-glucanase celB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
476		50510.49	4.0266

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SapiospermumIHEM14462	10920	N/A	2544	8376

• Gene Location: location=JOWA01000099:1923490-1924996(-)

Full Sequence      

MSSKIAAALPYITYLCAVASAQQIGTAIPEVHPRFPTFHCTTDGGCVEKQTSLVTDALAR
SFHAVDNMDISCGSKPFDATICPDEATCAQNCALEGIDYASIGVLTRGTSLTMRQYLFNG
TEYNRVSPRVYLLAEDEETYEMMKLLNQEISYDVDVSQLACGMNGALYLSEMDATGSKSA
TNVAGAAYGTGYCDAQCFDVPWINGLPNMNNSGACCNEMDIWEANSRATVYTPHTCSGVG
SFLCSGDECEFEGVCDKNGCGINPYRSGFQNYYAPGSDQVDTSRAFTVVTQFISDDGTAT
GTLSEIRRLYVQDGKVIENHPVSSDEDTPGFLTEEFCTDNNAEGFLRLGGMKTMGESLQR
GMVLIFSIWNSDGDFMDWLDSGEAGPCAEGEGDPKKILMERPDVSVTFSNVRWGDIGATF
DAVADVPGLVAAAPAMAQPAAAGAAGPPGVVTAAASGRYAPSLAVGVLALAFAVLI

Enzyme Prediction     

EC	3.2.1.4:18	3.2.1.73:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH7	23	420	7.4e-157	0.9951807228915662

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
153432	GH7_CBH_EG	0.0	29	414	1	386	Glycosyl hydrolase family 7. Glycosyl hydrolase family 7 contains eukaryotic endoglucanases (EGs) and cellobiohydrolases (CBHs) that hydrolyze glycosidic bonds using a double-displacement mechanism. This leads to a net retention of the conformation at the anomeric carbon. Both enzymes work synergistically in the degradation of cellulose,which is the main component of plant cell wall, and is composed of beta-1,4 linked glycosyl units. EG cleaves the beta-1,4 linkages of cellulose and CBH cleaves off cellobiose disaccharide units from the reducing end of the chain. In general, the O-glycosyl hydrolases are a widespread group of enzymes that hydrolyze the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycoside hydrolase family 7.
395677	Glyco_hydro_7	0.0	24	419	1	434	Glycosyl hydrolase family 7.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUS45843.1|GH7	4.58e-196	1	433	1	428
CDP28752.1|GH7	1.20e-171	17	420	18	431
CAP67010.1|GH7	1.20e-171	17	420	18	431
VBB80398.1|GH7	3.43e-171	19	420	20	431
CAJ75962.1|GH7	2.08e-165	17	420	13	412

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6SU8_A	3.71e-165	23	420	2	395	Chain A, Glucanase [Rasamsonia emersonii],6SU8_B Chain B, Glucanase [Rasamsonia emersonii],6SU8_C Chain C, Glucanase [Rasamsonia emersonii]
2A39_A	8.38e-134	30	420	8	398	HUMICOLA INSOLENS ENDOCELLULASE EGI NATIVE STRUCTURE [Humicola insolens],2A39_B HUMICOLA INSOLENS ENDOCELLULASE EGI NATIVE STRUCTURE [Humicola insolens]
6YOZ_AAA	8.96e-134	30	420	8	398	Chain AAA, Endoglucanase 1 [Humicola insolens],6YOZ_BBB Chain BBB, Endoglucanase 1 [Humicola insolens]
6YP1_AAA	9.58e-134	30	420	8	398	Chain AAA, Endoglucanase 1 [Humicola insolens]
1OJI_A	5.46e-133	30	420	8	398	Anatomy of glycosynthesis: Structure and kinetics of the Humicola insolens Cel7B E197A and E197S glycosynthase mutants [Humicola insolens],1OJJ_A Anatomy of glycosynthesis: Structure and kinetics of the Humicola insolens Cel7BE197A and E197S glycosynthase mutants [Humicola insolens],1OJJ_B Anatomy of glycosynthesis: Structure and kinetics of the Humicola insolens Cel7BE197A and E197S glycosynthase mutants [Humicola insolens],1OJK_A Anatomy of glycosynthesis: Structure and kinetics of the Humicola insolens Cel7BE197A and E197S glycosynthase mutants [Humicola insolens],1OJK_B Anatomy of glycosynthesis: Structure and kinetics of the Humicola insolens Cel7BE197A and E197S glycosynthase mutants [Humicola insolens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0CC84|CELB_ASPTN	1.50e-159	10	420	7	415	Probable endo-beta-1,4-glucanase celB OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=celB PE=3 SV=1
sp|Q2TX26|CELB_ASPOR	1.30e-154	10	420	7	414	Endo-beta-1,4-glucanase celB OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=celB PE=1 SV=1
sp|B8NW70|CELB_ASPFN	1.30e-154	10	420	7	414	Probable endo-beta-1,4-glucanase celB OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=celB PE=3 SV=1
sp|A1CG87|CELB_ASPCL	7.42e-152	15	420	12	412	Probable endo-beta-1,4-glucanase celB OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=celB PE=3 SV=1
sp|Q5B7R2|CELB_EMENI	5.08e-145	12	422	7	429	Endo-beta-1,4-glucanase celB OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=celB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000406	0.999582	CS pos: 21-22. Pr: 0.9675

TMHMM  Annotations     

There is no transmembrane helices in SAPIO_CDS5959-t41_1-p1.