CAZyme Information: SAPIO_CDS3617-t41_1-p1

Basic Information

• Species: Scedosporium apiospermum
• Lineage: Ascomycota; Sordariomycetes; ; Microascaceae; Scedosporium; Scedosporium apiospermum
• CAZyme ID: SAPIO_CDS3617-t41_1-p1
• CAZy Family: GH10
• CAZyme Description: Dolichyl-phosphate-mannose--protein mannosyltransferase 4

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
774		88556.71	9.3717

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SapiospermumIHEM14462	10920	N/A	2544	8376

• Gene Location: location=JOWA01000088:2299240-2301739(+)

Full Sequence      

MASGVPQGSLRQRNVPRKSVSPAPPAARSDDDVLDTLAKTKAKPAPSETSYRVAFGLITG
LAFLTRFWGISHPNEVVFDEVHFGKFASYYLERTYFFDVHPPFGKLLFAFMGWLVGYDGH
FHFDNIGDSYIANNIPYVAFRSLPAILGALTVSVVYLIMWESGYSLPACLLAAGLVLLDN
AHIGQTRLILLDATLVFSMACSLLFYIRFYKLRHEPFSRKWWKWLILTGFALSCDISTKY
VGTFAFVTIGSAVIIDLWDLLDIKRPGGALPMRLFIKHFCARAFGLIIMPFLFYLFWFQV
HFTVLSRSGPGDDFMTPEFQETLSDNVMLANAVTIDYYDTISIRHKETKTYLHSHPDRYP
LRYDDGRVSSQGQQVTGYPYNDTNNYWQIIPLQDDHKAGRHVKNHDLVRLRHIGTDTILL
SHDVASPYYPTNQEFTTVPLADAYGSRLNDTLFEIRIEHGKNKQEFKSISGHFKLIHNPS
KVAMWTHTKPLPDWGHRQQEINGNKQIAPSSNIWFVEDIPSLPADDKRREKPARKVKTLP
FLRKWFELQRSMFWHNSQLTSSHPYSSHPYQWPFLLRGVSFWTQNETRQQIYFLGNPIGW
WFASSLLAVFAGIVAADQLSLRRGIDALDRRTRSRFYNSTGFFFLAWATHYFPFWVMGRQ
LFLHHYLPAHLASCLVAGALVEFIFCSEPAPEEPTYQAVKSGKKPAPGPKHFVTAGERLA
NQSMVSTWIASGVVLAIVVAGWYFFLPLTYGYPGLTVEQVQQRKWLGYDLHFAK

Enzyme Prediction     

EC	2.4.1.109:8

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	58	302	1.1e-71	0.9910313901345291

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224839	PMT1	0.0	31	774	3	699	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
396786	PMT	2.57e-83	57	306	2	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
406576	PMT_4TMC	3.04e-78	543	766	1	197	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
397103	MIR	4.40e-18	353	501	13	170	MIR domain. The MIR (protein mannosyltransferase, IP3R and RyR) domain is a domain that may have a ligand transferase function.
197746	MIR	7.19e-07	337	391	6	56	Domain in ryanodine and inositol trisphosphate receptors and protein O-mannosyltransferases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UQC80449.1|GT39	0.0	3	774	5	773
AEO65833.1|GT39	0.0	2	774	3	777
UPK98559.1|GT39	0.0	3	774	4	768
AEO54171.1|GT39	0.0	2	774	3	775
CDP23606.1|GT39	0.0	1	774	1	773

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6P25_A	4.61e-98	57	769	52	728	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_A Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]
6P25_B	2.19e-88	7	678	20	696	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_B Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q59X23|PMT4_CANAL	3.15e-249	44	774	40	755	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PMT4 PE=2 SV=2
sp|O42933|PMT4_SCHPO	6.98e-241	14	766	31	767	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ogm4 PE=3 SV=1
sp|P46971|PMT4_YEAST	2.13e-238	14	774	2	762	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PMT4 PE=1 SV=1
sp|Q9Y6A1|POMT1_HUMAN	1.83e-106	43	684	7	684	Protein O-mannosyl-transferase 1 OS=Homo sapiens OX=9606 GN=POMT1 PE=1 SV=3
sp|Q8R2R1|POMT1_MOUSE	4.90e-106	58	669	44	669	Protein O-mannosyl-transferase 1 OS=Mus musculus OX=10090 GN=Pomt1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999873	0.000110

TMHMM  Annotations     

Start	End
51	70
95	117
138	160
188	210
283	305
598	615
636	658
724	746