CAZyme Information: SAPIO_CDS1784-t41_1-p1

Basic Information

• Species: Scedosporium apiospermum
• Lineage: Ascomycota; Sordariomycetes; ; Microascaceae; Scedosporium; Scedosporium apiospermum
• CAZyme ID: SAPIO_CDS1784-t41_1-p1
• CAZy Family: AA9
• CAZyme Description: UDP-glucose:Glycoprotein Glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1536		171310.97	4.8695

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SapiospermumIHEM14462	10920	N/A	2544	8376

• Gene Location: location=JOWA01000077:451655-456426(-)

Full Sequence      

MKFQGLSLSLTAVLLAAVPVSAVPSISVSMRAAFQAGPYLLELIETAAAENSTSYFPLLD
RVASGYFSGAATEKELYDRFLKLLVQDGHISEPEVLSTFKLALSMRSAAPRIEAHYQYYS
TGVQPRFLEPLPSACDTWVLFNGSQYCDYGFGEPRRLVADAIHTSTSLPFDRVLGHGPHA
ILYADVVSSPSFAPFHNSLVEKARRGEISYTVRYRRTNGPDENDTPLPVSGYGVELALKK
TDYIVIDDRDAASDGGGRGHDANHGSGSQVVLDEQEEVADLRPLSKAEVSSLGLNAASLI
MKGENPFETLIRFTQDFPAYSARIAAHNASLEFIEEQRDNLGTVVPQGVNVLWMNGVQLI
ERQIEPFSLVDMLRRERKLITEAKSIGLTGREAVALLGHPNVTSAKVDGSPQRFDWRDNQ
EGGEVIMWLNDLEADSQYDDFPTSITALLQRTYPGQVPRIRRNIFNAVIPVDVANVDDLK
VALQVLAYVNRLLPVRFGIVPFSSSEDSRNLRKVLYYLHDNYGLEGLFTYVELAIHGGQT
VADSAIFEEAIAAGSPAEGFKPSTFEEVLASKPLTERLQHVDMWVSRLNANSPTLPVFVN
GMVVPRVDNWLQGMGMKINSDLQMLRKGLQLGDINEDTRILGLFLDEAVKRRHPLIYADD
EKALNMIDLGKVYREHAALLQSVPVLDAYSDSSKETWAALTVIADMTTEDGLNLLYSALK
FKANNPGVRMDILHNPKASSNAAAAAINAALATNLDKLLEAGSIEEIRSLLPAQDPVPDT
QYGARLSAYIASTGLEPGTKGLVLNGRVVGPISQDEAFAETEFQQLLDFEQKNRILPVYA
AVADLGLGAVLSGPLAAARLTCLAARSTISDLPEGIFESAPTLRTSIFNTWKSKHTTIEV
GEAESATVHIIGILNPASEQGQRWTPILKVLSQVDGVYIKLFLNPEERLEELPIKRFYRY
VLDSEPTFDESGRVKPLDATFRGLPSEALLTVGMDVPPAWLVAAKSSVHDPDNIKLSLVK
GNVDAVYELRNILIEGHSRMVTKDAAPPSGAQLVLGTEKDPHSSDTIIMANLGYFQFKAN
PGFYNIQLQEGRSSQIFEIESVGAHGYDASPGDEGTEVALMDFKGTTLYPRLERKPGMET
ADVLSYGAEEAKPESDSLLSRGFRAAAELLGGSDSKHALEKKDKSSDDQAEINIFSVASG
HLYERMLNIMMLSVMKHTNHTVKFWFIEQFLSPSFKDSIPHLAKEYGFKYEMVTYKWPHW
LRQQKEKQREIWGYKILFLDVLFPLSLDKVIFVDADQIVRADMMELVNLDLKGAPYGFTP
MCDSRTEMEGFRFWKQGYWANYLRGLPYHISALYVVDLRRFRELAAGDRLRQQYHTLSAD
PNSLANLDQDLPNHMQFVIPIHSLPQEWLWCETWCSDESLRDAKTIDLCNNPMTKEPKLD
RARRQVPEWTVYDDEIANFERRRKGLPAVGDQKETPSVQEQEQIKEKEKEEEEARGKEEK
DGSDNDQPKPDEEPTFERNTKSRNWEEPLTRIKDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1192	1439	3.3e-135	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133054	GT8_HUGT1_C_like	0.0	1192	1439	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408203	Glyco_transf_24	0.0	1192	1459	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
408201	Thioredoxin_14	8.83e-82	414	656	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	4.64e-62	1035	1145	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
408199	Thioredoxin_12	1.43e-59	38	222	3	184	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UQC87132.1|GT24	0.0	4	1536	69	1551
UPK89075.1|GT24	0.0	5	1477	10	1449
QKD49569.1|GT24	0.0	6	1481	4	1456
CZS73929.1|GT24	0.0	15	1481	13	1454
CEF85276.1|GT24	0.0	15	1481	13	1454

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	20	1462	5	1450	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	20	1462	5	1450	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	0.0	20	1462	5	1450	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	20	1462	5	1216	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	0.0	24	1401	2	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	5.81e-272	26	1458	22	1423	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	6.29e-230	38	1456	42	1504	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q9JLA3|UGGG1_RAT	9.01e-224	25	1460	46	1524	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	2.46e-223	25	1460	46	1524	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9NYU2|UGGG1_HUMAN	2.84e-222	25	1462	46	1526	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000201	0.999758	CS pos: 22-23. Pr: 0.9798

TMHMM  Annotations     

There is no transmembrane helices in SAPIO_CDS1784-t41_1-p1.