CAZyme Information: SAPIO_CDS0118-t41_1-p1

Basic Information

• Species: Scedosporium apiospermum
• Lineage: Ascomycota; Sordariomycetes; ; Microascaceae; Scedosporium; Scedosporium apiospermum
• CAZyme ID: SAPIO_CDS0118-t41_1-p1
• CAZy Family: AA1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
514	JOWA01000011|CGC1	56234.12	4.5797

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_SapiospermumIHEM14462	10920	N/A	2544	8376

• Gene Location: location=JOWA01000011:446061-447745(-)

Full Sequence      

MRRLTIVGGLLAVLTPTAFALSNYISLEACLKASSVPFDKPNSCAWNLDVAPFNERLPYT
PVAIAVPRTIAHIEGAVKCASEFGVKVSAKCGGHSYASLGLGGEDGHLVVEMDRMHEVRL
SEDGETAVIQGGARLGHVATELWEKGRRAISHGTCPGVGISGHALHGGFGMSSYTHGLAL
DWIISATVVLANGTTVRASEDENPDLFWALRGAGGSMGIVAEYEFATFEPSEEYTHFEVT
LDWPDAESIIGGWLELQAWGEEEMPREMNMRVSVDTRGVRLDGLYHGGQADMEAVVLPLL
ERLGGGEIALNVTYDWVGQLEQYAFAEDLNLTHPYNQHETFYAKSLFTHALPPEAIASFM
SYTLTNATAALRQTPSPALDSDSDSPSPVPLRYWWILIDIHGGANSYIAREVAHNSTSYA
HRDKLLLFQFYDRSFGEYPSGEEPFGLLDGFVDSITGHLGEGQWGMYVNYADPRVEDVAE
EVYWGKNVERLREVKRAVDPGDLFFNPLGIRSAA

Enzyme Prediction     

EC	1.1.3.-:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
AA7	51	510	1.3e-88	0.9890829694323144

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396238	FAD_binding_4	3.39e-26	61	199	1	139	FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidizes the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan.
223354	GlcD	2.75e-25	58	513	29	459	FAD/FMN-containing dehydrogenase [Energy production and conversion].
369658	BBE	3.41e-11	467	511	2	45	Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine.
273751	FAD_lactone_ox	5.98e-10	67	220	21	171	sugar 1,4-lactone oxidases. This model represents a family of at least two different sugar 1,4 lactone oxidases, both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862; OX2_COVAL_FAD; 1.
215242	PLN02441	7.56e-06	60	220	64	231	cytokinin dehydrogenase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEO62186.1|AA7	8.01e-162	14	510	2	488
ANF89368.1|AA7	3.41e-159	26	513	26	495
AEO58513.1|AA7|1.1.3.-	3.41e-159	26	513	26	495
CAP69207.1|AA7	3.59e-156	11	511	14	495
CDP32688.1|AA7	3.59e-156	11	511	14	495

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5K8E_A	1.18e-160	26	513	26	495	Xylooligosaccharide oxidase from Myceliophthora thermophila C1 [Thermothelomyces thermophilus ATCC 42464],5L6F_A Xylooligosaccharide oxidase from Myceliophthora thermophila C1 in complex with Xylobiose [Thermothelomyces thermophilus ATCC 42464],5L6G_A Xylooligosaccharide oxidase from Myceliophthora thermophila C1 in complex with Xylose [Thermothelomyces thermophilus ATCC 42464]
1ZR6_A	1.57e-138	22	514	4	480	The crystal structure of an Acremonium strictum glucooligosaccharide oxidase reveals a novel flavinylation [Sarocladium strictum],2AXR_A Crystal structure of glucooligosaccharide oxidase from Acremonium strictum: a novel flavinylation of 6-S-cysteinyl, 8alpha-N1-histidyl FAD [Sarocladium strictum]
3HSU_A	3.56e-137	22	514	4	480	Chain A, Glucooligosaccharide oxidase [Sarocladium strictum]
6Y0R_AAA	1.00e-136	26	514	26	492	Chain AAA, Chitooligosaccharide oxidase [Fusarium graminearum PH-1]
3RJ8_A	4.61e-130	26	511	2	470	Crystal structure of carbohydrate oxidase from Microdochium nivale [Microdochium nivale],3RJA_A Crystal structure of carbohydrate oxidase from Microdochium nivale in complex with substrate analogue [Microdochium nivale]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|G2QG48|XYLO_MYCTT	6.05e-160	26	513	26	495	Xylooligosaccharide oxidase OS=Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) OX=573729 GN=xylO PE=1 SV=1
sp|Q6PW77|GOOX_SARSR	1.00e-137	26	514	27	499	Glucooligosaccharide oxidase OS=Sarocladium strictum OX=5046 GN=gluO PE=1 SV=1
sp|I1S2K2|CHITO_GIBZE	5.15e-136	26	514	26	492	Chitooligosaccharide oxidase OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=chitO PE=1 SV=1
sp|I1SB12|MNCO_MICNN	1.50e-130	11	511	9	492	Carbohydrate oxidase OS=Microdochium nivale OX=5520 GN=MnCO PE=1 SV=2
sp|E9F5F1|SUBF_METRA	7.65e-123	26	514	28	493	FAD-linked oxidoreductase subF OS=Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) OX=655844 GN=subF PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000828	0.999140	CS pos: 20-21. Pr: 0.9739

TMHMM  Annotations     

There is no transmembrane helices in SAPIO_CDS0118-t41_1-p1.