CAZyme Information: RVD89818.1

Basic Information

• Species: Arthrobotrys flagrans
• Lineage: Ascomycota; Orbiliomycetes; ; Orbiliaceae; Arthrobotrys; Arthrobotrys flagrans
• CAZyme ID: RVD89818.1
• CAZy Family: GT61
• CAZyme Description: GH16 domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A437AF81]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
760		83542.12	4.7150

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AflagransCBSH-5679	9927	N/A	30	9897

• Gene Location: location=SAEB01000001:3218773-3221293(-)

Full Sequence      

MDERTPPADGSGSQPAWGIDNGVERALGKVVDEVEAGQERPEQKETLLGDTIAAPAPIRP
APAPVQPPQQSIWAKKATRLKKRGGSFRSESGLPLPPAPPQAHRHSYTPVVPSPLNPASS
GSEDSGSGRPSHSSSSVHQDALVAPSVSLSNSVSTETGPKSSRLLLSPEPIMPTASLMDL
GREYDRYPARTPRNSIMLLQKTPSDTPSQRHSNPLLGETSSAEHKSESTEIDNEKFFNPY
SESITKPTFLNAGFPLYMDEKEPDDYMHNPSEGDKELPNHSLKDIGLRGFLSFCCFWIFV
CGLLALFIVLPVLTFTGYVNTPGETENPHETEWQMQPWEYVETKYSFPILGGIRTSMVDP
ETPEFAKTRKATDGTTYKLVFSDEFNTDGRSFYEGDDQFWTAPDLHYAATNDLEWYDPDA
VTTAAGTLRLKLDAWKNHDLNYRSGMLQSWNKLCFKGGILEVSASLPGPGGKMGLWPGIW
TLGNLARPGYLATSEGTWPYTYDECDIGITPNQSSPDGISFLPGQRLNSCTCKGEDHPNP
GVGRGAPEIDALEGSVAGGINIGVASQSSQIAPYDVWYIPDYEFLEIHNHSVTEMNSWCG
GPFQQAISGITALNNEWYDGKNFQKYAFEYVPGKGTGHITWFVGDEPTFTMKGESTGPNG
NIGARHISQEPMSIILNLGVSNSWAYIDWPGLEFPTTMYIDYVRIYQPEGSEMVTCDPPG
YPTTDYIKKHMNAYTNPNITSWSKAGYSFPKNTLMNGCSK

Enzyme Prediction     

No EC number prediction in RVD89818.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	383	706	5.3e-159	0.9969325153374233

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	0.0	254	758	1	500	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	1.29e-170	379	707	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	4.35e-85	343	720	15	355	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	1.62e-27	381	706	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185691	GH16_Strep_laminarinase_like	1.75e-06	377	707	2	259	Streptomyces laminarinase-like, member of glycosyl hydrolase family 16. Proteins similar to Streptomyces sioyaensis beta-1,3-glucanase (laminarinase) present in Actinomycetales as well as Peziomycotina. Laminarinases belong to glycosyl hydrolase family 16 and hydrolyze the glycosidic bond of the 1,3-beta-linked glucan, a major component of fungal and plant cell walls and the structural and storage polysaccharides (laminarin) of marine macro-algae. Members of the GH16 family have a conserved jelly roll fold with an active site channel.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
EGX46455.1|GH16_2	0.0	1	693	4	696
CDR41262.1|GH16_2	9.01e-227	183	758	104	674
CCE78661.1|GH16_2	5.42e-225	166	758	105	692
CAR24695.1|GH16_2	1.37e-224	249	758	191	700
QLL32994.1|GH16_2	1.60e-224	135	758	116	715

PDB Hits     

RVD89818.1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P33336|SKN1_YEAST	2.91e-218	176	759	176	764	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P32486|KRE6_YEAST	8.70e-218	183	759	151	712	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P87024|SKN1_CANAX	3.48e-216	254	758	238	737	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P87023|KRE6_CANAX	2.18e-215	183	758	158	740	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|O13941|YEPB_SCHPO	1.33e-198	261	759	131	628	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000090	0.000000

TMHMM  Annotations     

Start	End
293	315