CAZyme Information: RVD89346.1

Basic Information

• Species: Arthrobotrys flagrans
• Lineage: Ascomycota; Orbiliomycetes; ; Orbiliaceae; Arthrobotrys; Arthrobotrys flagrans
• CAZyme ID: RVD89346.1
• CAZy Family: GT3
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1587	SAEB01000001|CGC12	179348.05	5.4953

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AflagransCBSH-5679	9927	N/A	30	9897

• Gene Location: location=SAEB01000001:1440318-1445257(-)

Full Sequence      

MWFSTLRTQQSSRRVLLSGVLALASLQGVYARSQPQDIEEPGTVKQWEGLNLSLKTPWNG
GPYILEILECAGLEEPEFYFTLLDLIKTRGINIADSDKEMYDTVVERVEQQQLLDHRFIP
TWKFCISLRETAPRIEAQYQFYSNSVTFPKGKSCPVWFDDGERQSCNPEDISKVKPGSSS
ASKSIAFDRQNKEDTRKGLVTFYIDIIDKGFPQAYSEMTAAADKAGIAYKVRYKPSPSLK
NPKPVGLSGYGVGLSLKKTDYLVIDDRGAKEGSASASDASSTESSAKPSATYKFRTPENL
KPGEKRFPLWDLEDVDDPVRDLRDLLPLSQTNVGDIGFKTANFITKSGIFPPDMLMKILS
DYPKYAHTIGTMGGVLNEWMEEHSENREKFVPPGYNIMFLNGMALDPSEVTATNLIKIMR
RERHHGIQIRLLGLNQQETLDLLAHENITNAKMTPDEPRFDFREQEGEENAIIWLNDLAI
DERYKNWPDSIKEILIPQYGGQLAAVRKNIHTVILPLDFSNREDLTLIAERLNFFVTRDL
ALTIGIVPVVRNNLGSANSRIFRALLESYDLETAVSYIKDGLEADKHGVRYPAPEYVHHA
IKGRTRVEGAPEVSLSAVMESKEFQAYARAAGNWESRFSVTSRPYPFVVNGIVDFFSDEW
VRTLSEALPKDVKRMQEAIEKGTLTDNDNIQDFLLDNPLHKRNVAIFPEDGKVQLINLAN
LFQSNPEIFENLPHFPGKLVEEEETSSADVWVIGDFDTESGYNLLAEASKLQASRPGISI
TAISNTGEKTNVQTVSALLHSLVGNLEVLRNNDLLQKIIEEIKPQKDYIDLKVNGQKVFG
GDAKTEGWALPDKVAAEKFWSEAAAVVERKFGVKPGQRAIVVNGRLVGPFEEGVSWTKDD
IETLVRVELKARITPILNAAKSLDVLPRLRAANYKARLTSTVSELLDGENTSLSSGRMTV
RTNKPMGWKRGKTVLTAGDPIWSTFRFVVSLDPVSEVGQKWAPILRVLMSMDGVGIYTFL
NPNDNLREVPIKRYYRHVLSSQPKFDENGDILDPKAQFIGLPDQTLYSLSMDVPPSWLVT
PSETIHDLDNIKLQTFRERFGTSDLNATYVLKNILIEGHSRDTTLNEPSAGAQLELGTPE
NPVMQDTIIMSNLGYFQFKSNPGHWQIRLKPGRSSDIFSIQSLGISGFTTSSNAEDDVKD
ITLMSLNGVTLFPRLKRNKGYNKVDVHAPLGSTESTVEKATGFAEGLLAKLGLGKKKETG
LAKAPAEINIFSVASGHLYERFLNIMMVSVMRHTKHTVKFWFIENFLSPAFKDFLPVMAK
EYNFDYELVTYKWPHWLRAQKEKQREIWGYKILFLDVLFPLNLDKIIFVDADQIVRTDLK
ELVDLDLEGAVYGFTPMCDSRLEIEGYRFWKQGYWKQMLGNLKYHISALFVVDLKVFRQL
AAGDRLRQQYHQLSADPNSLSNLDQDLPNNMQRQLPIFSLPQDWLWCETWCSDESFKTAK
TIDLCNNPMTKEPKLDRARRQIPEWTEYDDEVAALAKKVQAKGVENMREALVQKEALGEQ
EKAVKEESSEEKKEEKVADTEKPKDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1268	1515	1.9e-133	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133054	GT8_HUGT1_C_like	0.0	1268	1515	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408203	Glyco_transf_24	0.0	1268	1535	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
408201	Thioredoxin_14	4.14e-68	460	706	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
132996	Glyco_transf_8	4.48e-61	1268	1515	1	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
399437	UDP-g_GGTase	3.01e-52	1117	1226	1	107	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
EGX46010.1|GT24	0.0	1	1587	1	1587
QDS71787.1|GT24	0.0	50	1538	31	1465
QSZ31337.1|GT24	0.0	50	1538	32	1455
UPK89075.1|GT24	0.0	50	1535	26	1435
UJO21924.1|GT24	0.0	50	1538	22	1495

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	40	1539	1	1451	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	40	1539	1	1451	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	0.0	40	1539	1	1451	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	6.76e-311	50	1477	4	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	4.94e-286	40	1539	1	1217	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	1.79e-247	50	1535	22	1424	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	2.72e-221	48	1535	45	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q6P5E4|UGGG1_MOUSE	6.74e-221	48	1535	45	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	1.32e-220	48	1535	45	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	4.14e-214	54	1540	34	1512	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.006545	0.993432	CS pos: 31-32. Pr: 0.9617

TMHMM  Annotations     

There is no transmembrane helices in RVD89346.1.